Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4625 and Psyr_4626 overlap by 4 nucleotides Psyr_4626 and Psyr_4627 overlap by 4 nucleotides Psyr_4627 and Psyr_4628 are separated by 95 nucleotides Psyr_4628 and Psyr_4629 overlap by 1 nucleotides
Psyr_4625: Psyr_4625 - PpiC-type peptidyl-prolyl cis-trans isomerase, at 5,485,325 to 5,486,653
_4625
Psyr_4626: Psyr_4626 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 5,486,650 to 5,487,639
_4626
Psyr_4627: Psyr_4627 - dimethyladenosine transferase, at 5,487,636 to 5,488,442
_4627
Psyr_4628: Psyr_4628 - Protein of unknown function DUF525, at 5,488,538 to 5,488,939
_4628
Psyr_4629: Psyr_4629 - Bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 5,488,939 to 5,489,814
_4629
Position (kb) 5487
5488
5489 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5487.649 kb on - strand at 5487.649 kb on - strand at 5487.649 kb on - strand at 5487.972 kb on + strand, within Psyr_4627 at 5487.972 kb on + strand, within Psyr_4627 at 5487.972 kb on + strand, within Psyr_4627 at 5488.123 kb on - strand, within Psyr_4627 at 5488.354 kb on + strand, within Psyr_4627 at 5488.355 kb on - strand, within Psyr_4627 at 5488.457 kb on + strand at 5488.457 kb on + strand at 5488.457 kb on + strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.458 kb on - strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.488 kb on + strand at 5488.489 kb on - strand at 5488.489 kb on - strand at 5488.489 kb on - strand at 5488.489 kb on - strand at 5488.489 kb on - strand at 5488.489 kb on - strand at 5488.498 kb on - strand at 5488.498 kb on - strand at 5488.507 kb on + strand at 5488.507 kb on + strand at 5488.507 kb on + strand at 5488.508 kb on - strand at 5488.508 kb on - strand at 5488.508 kb on - strand at 5488.537 kb on + strand at 5488.538 kb on - strand at 5488.553 kb on + strand at 5488.553 kb on + strand at 5488.553 kb on + strand at 5488.683 kb on + strand, within Psyr_4628 at 5488.683 kb on + strand, within Psyr_4628 at 5488.683 kb on + strand, within Psyr_4628 at 5488.801 kb on + strand, within Psyr_4628 at 5488.801 kb on + strand, within Psyr_4628 at 5488.802 kb on - strand, within Psyr_4628 at 5488.802 kb on - strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.805 kb on + strand, within Psyr_4628 at 5488.817 kb on - strand, within Psyr_4628 at 5488.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 5,487,649 - +1.8 5,487,649 - +0.2 5,487,649 - +0.8 5,487,972 + Psyr_4627 0.42 -2.0 5,487,972 + Psyr_4627 0.42 -1.2 5,487,972 + Psyr_4627 0.42 -0.4 5,488,123 - Psyr_4627 0.60 -1.1 5,488,354 + Psyr_4627 0.89 -1.7 5,488,355 - Psyr_4627 0.89 -1.0 5,488,457 + -0.7 5,488,457 + -3.1 5,488,457 + +0.8 5,488,458 - -0.1 5,488,458 - +0.4 5,488,458 - -0.4 5,488,458 - +2.8 5,488,458 - -0.8 5,488,458 - -1.4 5,488,458 - -1.1 5,488,458 - +2.8 5,488,488 + +0.3 5,488,488 + +1.0 5,488,488 + -0.6 5,488,488 + -1.9 5,488,488 + -0.0 5,488,488 + -0.8 5,488,488 + -0.2 5,488,489 - -0.7 5,488,489 - -0.8 5,488,489 - -1.4 5,488,489 - -1.1 5,488,489 - -0.4 5,488,489 - -1.6 5,488,498 - -0.7 5,488,498 - -0.3 5,488,507 + +1.2 5,488,507 + -1.8 5,488,507 + -0.4 5,488,508 - +0.4 5,488,508 - +1.2 5,488,508 - -0.7 5,488,537 + -1.5 5,488,538 - -1.6 5,488,553 + -1.4 5,488,553 + +0.5 5,488,553 + +0.5 5,488,683 + Psyr_4628 0.36 -0.5 5,488,683 + Psyr_4628 0.36 -0.3 5,488,683 + Psyr_4628 0.36 -0.1 5,488,801 + Psyr_4628 0.65 +1.2 5,488,801 + Psyr_4628 0.65 +0.2 5,488,802 - Psyr_4628 0.66 -0.6 5,488,802 - Psyr_4628 0.66 +0.1 5,488,805 + Psyr_4628 0.66 +0.0 5,488,805 + Psyr_4628 0.66 -1.3 5,488,805 + Psyr_4628 0.66 -1.1 5,488,805 + Psyr_4628 0.66 +1.2 5,488,805 + Psyr_4628 0.66 +0.2 5,488,805 + Psyr_4628 0.66 -1.1 5,488,805 + Psyr_4628 0.66 +1.2 5,488,805 + Psyr_4628 0.66 +1.2 5,488,805 + Psyr_4628 0.66 -0.3 5,488,817 - Psyr_4628 0.69 -2.7 5,488,949 - -3.1
Or see this region's nucleotide sequence