Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2997 and Psyr_2998 overlap by 4 nucleotides Psyr_2998 and Psyr_2999 are separated by 13 nucleotides Psyr_2999 and Psyr_3000 overlap by 4 nucleotides
Psyr_2997: Psyr_2997 - Glycoside hydrolase, family 13, N-terminal:Alpha amylase, catalytic region, at 3,592,521 to 3,594,704
_2997
Psyr_2998: Psyr_2998 - conserved hypothetical protein, at 3,594,701 to 3,595,714
_2998
Psyr_2999: Psyr_2999 - LmbE-like protein, at 3,595,728 to 3,596,501
_2999
Psyr_3000: Psyr_3000 - methyltransferase, putative, at 3,596,498 to 3,597,124
_3000
Position (kb)
3594
3595
3596 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3593.973 kb on + strand, within Psyr_2997 at 3594.108 kb on - strand, within Psyr_2997 at 3594.342 kb on + strand, within Psyr_2997 at 3594.342 kb on + strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.343 kb on - strand, within Psyr_2997 at 3594.576 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.645 kb on + strand at 3594.646 kb on - strand at 3594.646 kb on - strand at 3594.646 kb on - strand at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.778 kb on - strand at 3594.896 kb on + strand, within Psyr_2998 at 3594.897 kb on - strand, within Psyr_2998 at 3595.009 kb on + strand, within Psyr_2998 at 3595.263 kb on - strand, within Psyr_2998 at 3595.511 kb on + strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.512 kb on - strand, within Psyr_2998 at 3595.575 kb on - strand, within Psyr_2998 at 3595.720 kb on + strand at 3595.772 kb on + strand at 3595.773 kb on - strand at 3595.894 kb on + strand, within Psyr_2999 at 3595.894 kb on + strand, within Psyr_2999 at 3595.894 kb on + strand, within Psyr_2999 at 3595.895 kb on - strand, within Psyr_2999 at 3595.895 kb on - strand, within Psyr_2999 at 3595.970 kb on + strand, within Psyr_2999 at 3595.971 kb on - strand, within Psyr_2999 at 3596.375 kb on + strand, within Psyr_2999 at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.516 kb on + strand at 3596.517 kb on - strand at 3596.517 kb on - strand at 3596.573 kb on + strand, within Psyr_3000
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 3,593,973 + Psyr_2997 0.66 +1.9 3,594,108 - Psyr_2997 0.73 -0.9 3,594,342 + Psyr_2997 0.83 +0.6 3,594,342 + Psyr_2997 0.83 -0.4 3,594,343 - Psyr_2997 0.83 +1.0 3,594,343 - Psyr_2997 0.83 +1.7 3,594,343 - Psyr_2997 0.83 -1.2 3,594,343 - Psyr_2997 0.83 -0.9 3,594,576 + +1.1 3,594,645 + +0.1 3,594,645 + +0.1 3,594,645 + +0.6 3,594,645 + +0.9 3,594,646 - -3.0 3,594,646 - +2.1 3,594,646 - -1.1 3,594,778 - -1.4 3,594,778 - -0.9 3,594,778 - -0.1 3,594,896 + Psyr_2998 0.19 +0.2 3,594,897 - Psyr_2998 0.19 +0.7 3,595,009 + Psyr_2998 0.30 -0.2 3,595,263 - Psyr_2998 0.55 +3.1 3,595,511 + Psyr_2998 0.80 +1.0 3,595,512 - Psyr_2998 0.80 +1.0 3,595,512 - Psyr_2998 0.80 +2.5 3,595,512 - Psyr_2998 0.80 +0.5 3,595,512 - Psyr_2998 0.80 +0.2 3,595,512 - Psyr_2998 0.80 -0.4 3,595,575 - Psyr_2998 0.86 +1.1 3,595,720 + -1.5 3,595,772 + +0.6 3,595,773 - +0.4 3,595,894 + Psyr_2999 0.21 +1.5 3,595,894 + Psyr_2999 0.21 +0.0 3,595,894 + Psyr_2999 0.21 -1.3 3,595,895 - Psyr_2999 0.22 +0.2 3,595,895 - Psyr_2999 0.22 -0.8 3,595,970 + Psyr_2999 0.31 +0.4 3,595,971 - Psyr_2999 0.31 +0.5 3,596,375 + Psyr_2999 0.84 +0.1 3,596,516 + +0.3 3,596,516 + -0.7 3,596,516 + -0.4 3,596,516 + +1.0 3,596,516 + -0.2 3,596,516 + -0.6 3,596,517 - -0.7 3,596,517 - -0.4 3,596,573 + Psyr_3000 0.12 +0.6
Or see this region's nucleotide sequence