Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2398 and Psyr_2399 overlap by 4 nucleotides Psyr_2399 and Psyr_2400 are separated by 6 nucleotides Psyr_2400 and Psyr_2401 overlap by 16 nucleotides
Psyr_2398: Psyr_2398 - ABC transporter, at 2,780,195 to 2,781,349
_2398
Psyr_2399: Psyr_2399 - Binding-protein-dependent transport systems inner membrane component, at 2,781,346 to 2,782,161
_2399
Psyr_2400: Psyr_2400 - Binding-protein-dependent transport systems inner membrane component, at 2,782,168 to 2,783,124
_2400
Psyr_2401: Psyr_2401 - extracellular solute-binding protein, family 1, at 2,783,109 to 2,784,134
_2401
Position (kb)
2781
2782
2783 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2780.412 kb on - strand, within Psyr_2398 at 2780.745 kb on + strand, within Psyr_2398 at 2780.745 kb on + strand, within Psyr_2398 at 2780.745 kb on + strand, within Psyr_2398 at 2780.746 kb on - strand, within Psyr_2398 at 2781.060 kb on + strand, within Psyr_2398 at 2781.289 kb on + strand at 2781.354 kb on + strand at 2781.354 kb on + strand at 2781.354 kb on + strand at 2781.354 kb on + strand at 2781.355 kb on - strand at 2781.355 kb on - strand at 2781.355 kb on - strand at 2781.355 kb on - strand at 2781.611 kb on + strand, within Psyr_2399 at 2781.611 kb on + strand, within Psyr_2399 at 2781.612 kb on - strand, within Psyr_2399 at 2781.612 kb on - strand, within Psyr_2399 at 2781.612 kb on - strand, within Psyr_2399 at 2781.612 kb on - strand, within Psyr_2399 at 2781.612 kb on - strand, within Psyr_2399 at 2781.785 kb on + strand, within Psyr_2399 at 2782.112 kb on + strand at 2782.112 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.223 kb on + strand at 2782.224 kb on - strand at 2782.224 kb on - strand at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.364 kb on + strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.365 kb on - strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.553 kb on + strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.554 kb on - strand, within Psyr_2400 at 2782.676 kb on + strand, within Psyr_2400 at 2782.827 kb on + strand, within Psyr_2400 at 2782.827 kb on + strand, within Psyr_2400 at 2782.828 kb on - strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.874 kb on + strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.875 kb on - strand, within Psyr_2400 at 2782.918 kb on + strand, within Psyr_2400 at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.110 kb on + strand at 2783.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,780,412 - Psyr_2398 0.19 -2.1 2,780,745 + Psyr_2398 0.48 +0.1 2,780,745 + Psyr_2398 0.48 +1.2 2,780,745 + Psyr_2398 0.48 -0.1 2,780,746 - Psyr_2398 0.48 +0.1 2,781,060 + Psyr_2398 0.75 +0.1 2,781,289 + +2.8 2,781,354 + +0.4 2,781,354 + -0.5 2,781,354 + -1.2 2,781,354 + -0.2 2,781,355 - -0.9 2,781,355 - +0.7 2,781,355 - +0.7 2,781,355 - -0.4 2,781,611 + Psyr_2399 0.32 -0.6 2,781,611 + Psyr_2399 0.32 -1.1 2,781,612 - Psyr_2399 0.33 -0.4 2,781,612 - Psyr_2399 0.33 -1.5 2,781,612 - Psyr_2399 0.33 -0.5 2,781,612 - Psyr_2399 0.33 -1.1 2,781,612 - Psyr_2399 0.33 -0.4 2,781,785 + Psyr_2399 0.54 -0.5 2,782,112 + +1.6 2,782,112 + -1.1 2,782,223 + -0.5 2,782,223 + +0.2 2,782,223 + +0.3 2,782,223 + +0.2 2,782,224 - -1.4 2,782,224 - +0.0 2,782,364 + Psyr_2400 0.20 -0.1 2,782,364 + Psyr_2400 0.20 +0.1 2,782,364 + Psyr_2400 0.20 -0.3 2,782,364 + Psyr_2400 0.20 -0.7 2,782,364 + Psyr_2400 0.20 -1.6 2,782,364 + Psyr_2400 0.20 -0.5 2,782,364 + Psyr_2400 0.20 -0.9 2,782,364 + Psyr_2400 0.20 +1.0 2,782,365 - Psyr_2400 0.21 +3.5 2,782,365 - Psyr_2400 0.21 +0.1 2,782,365 - Psyr_2400 0.21 +0.4 2,782,365 - Psyr_2400 0.21 -2.0 2,782,553 + Psyr_2400 0.40 +0.4 2,782,553 + Psyr_2400 0.40 -0.0 2,782,553 + Psyr_2400 0.40 +0.6 2,782,553 + Psyr_2400 0.40 -2.1 2,782,553 + Psyr_2400 0.40 -0.0 2,782,553 + Psyr_2400 0.40 +1.1 2,782,553 + Psyr_2400 0.40 +1.2 2,782,553 + Psyr_2400 0.40 +0.3 2,782,553 + Psyr_2400 0.40 +1.4 2,782,554 - Psyr_2400 0.40 -1.1 2,782,554 - Psyr_2400 0.40 -0.2 2,782,554 - Psyr_2400 0.40 -1.8 2,782,554 - Psyr_2400 0.40 +0.8 2,782,554 - Psyr_2400 0.40 -0.5 2,782,676 + Psyr_2400 0.53 +0.4 2,782,827 + Psyr_2400 0.69 +0.4 2,782,827 + Psyr_2400 0.69 -2.0 2,782,828 - Psyr_2400 0.69 -0.4 2,782,874 + Psyr_2400 0.74 +1.0 2,782,874 + Psyr_2400 0.74 -0.7 2,782,874 + Psyr_2400 0.74 -0.4 2,782,875 - Psyr_2400 0.74 -0.2 2,782,875 - Psyr_2400 0.74 -0.1 2,782,875 - Psyr_2400 0.74 +1.4 2,782,918 + Psyr_2400 0.78 -2.0 2,783,110 + -1.0 2,783,110 + -0.7 2,783,110 + +0.8 2,783,110 + -0.0 2,783,111 - +0.4
Or see this region's nucleotide sequence