Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2254 and Psyr_2255 overlap by 4 nucleotides Psyr_2255 and Psyr_2256 are separated by 41 nucleotides Psyr_2256 and Psyr_2257 overlap by 7 nucleotides
Psyr_2254: Psyr_2254 - Phosphonate metabolism PhnJ, at 2,619,171 to 2,620,058
_2254
Psyr_2255: Psyr_2255 - ABC transporter, at 2,620,055 to 2,620,876
_2255
Psyr_2256: Psyr_2256 - ABC transporter, at 2,620,918 to 2,621,634
_2256
Psyr_2257: Psyr_2257 - Amidohydrolase, at 2,621,628 to 2,622,773
_2257
Position (kb)
2620
2621
2622 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2620.146 kb on - strand, within Psyr_2255 at 2620.146 kb on - strand, within Psyr_2255 at 2620.326 kb on - strand, within Psyr_2255 at 2620.713 kb on + strand, within Psyr_2255 at 2620.994 kb on + strand, within Psyr_2256 at 2621.098 kb on + strand, within Psyr_2256 at 2621.098 kb on + strand, within Psyr_2256 at 2621.098 kb on + strand, within Psyr_2256 at 2621.098 kb on + strand, within Psyr_2256 at 2621.099 kb on - strand, within Psyr_2256 at 2621.680 kb on + strand at 2622.171 kb on + strand, within Psyr_2257 at 2622.183 kb on + strand, within Psyr_2257 at 2622.183 kb on + strand, within Psyr_2257 at 2622.183 kb on + strand, within Psyr_2257 at 2622.183 kb on + strand, within Psyr_2257 at 2622.184 kb on - strand, within Psyr_2257 at 2622.184 kb on - strand, within Psyr_2257 at 2622.184 kb on - strand, within Psyr_2257 at 2622.184 kb on - strand, within Psyr_2257 at 2622.195 kb on + strand, within Psyr_2257 at 2622.196 kb on - strand, within Psyr_2257 at 2622.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,620,146 - Psyr_2255 0.11 -1.2 2,620,146 - Psyr_2255 0.11 -1.8 2,620,326 - Psyr_2255 0.33 -1.0 2,620,713 + Psyr_2255 0.80 -0.4 2,620,994 + Psyr_2256 0.11 -1.9 2,621,098 + Psyr_2256 0.25 -1.2 2,621,098 + Psyr_2256 0.25 -0.8 2,621,098 + Psyr_2256 0.25 +0.2 2,621,098 + Psyr_2256 0.25 +2.5 2,621,099 - Psyr_2256 0.25 -0.7 2,621,680 + -1.0 2,622,171 + Psyr_2257 0.47 -0.1 2,622,183 + Psyr_2257 0.48 +1.9 2,622,183 + Psyr_2257 0.48 -0.2 2,622,183 + Psyr_2257 0.48 -1.3 2,622,183 + Psyr_2257 0.48 -1.1 2,622,184 - Psyr_2257 0.49 +0.2 2,622,184 - Psyr_2257 0.49 +0.1 2,622,184 - Psyr_2257 0.49 +0.5 2,622,184 - Psyr_2257 0.49 +0.2 2,622,195 + Psyr_2257 0.49 +0.1 2,622,196 - Psyr_2257 0.50 +0.2 2,622,543 + -0.4
Or see this region's nucleotide sequence