Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2017 and Psyr_2018 are separated by 90 nucleotides Psyr_2018 and Psyr_2019 are separated by 520 nucleotides
Psyr_2017: Psyr_2017 - Heat shock protein Hsp90:ATP-binding region, ATPase-like protein, at 2,340,585 to 2,342,498
_2017
Psyr_2018: Psyr_2018 - Dienelactone hydrolase, at 2,342,589 to 2,343,317
_2018
Psyr_2019: Psyr_2019 - 3-oxoacyl-[acyl-carrier-protein] synthase I, at 2,343,838 to 2,345,061
_2019
Position (kb)
2342
2343
2344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2341.722 kb on + strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.723 kb on - strand, within Psyr_2017 at 2341.903 kb on - strand, within Psyr_2017 at 2341.905 kb on + strand, within Psyr_2017 at 2341.906 kb on - strand, within Psyr_2017 at 2341.924 kb on - strand, within Psyr_2017 at 2342.023 kb on - strand, within Psyr_2017 at 2342.312 kb on + strand at 2342.313 kb on - strand at 2342.313 kb on - strand at 2342.454 kb on + strand at 2342.483 kb on - strand at 2342.483 kb on - strand at 2342.643 kb on + strand at 2342.644 kb on - strand at 2342.644 kb on - strand at 2342.644 kb on - strand at 2342.660 kb on - strand at 2342.661 kb on + strand at 2342.662 kb on - strand, within Psyr_2018 at 2342.662 kb on - strand, within Psyr_2018 at 2342.662 kb on - strand, within Psyr_2018 at 2342.794 kb on - strand, within Psyr_2018 at 2342.816 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.935 kb on + strand, within Psyr_2018 at 2342.936 kb on - strand, within Psyr_2018 at 2342.936 kb on - strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.181 kb on + strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.182 kb on - strand, within Psyr_2018 at 2343.183 kb on + strand, within Psyr_2018 at 2343.183 kb on + strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.184 kb on - strand, within Psyr_2018 at 2343.189 kb on + strand, within Psyr_2018 at 2343.190 kb on - strand, within Psyr_2018 at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.249 kb on + strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.250 kb on - strand at 2343.750 kb on - strand at 2343.750 kb on - strand at 2343.750 kb on - strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.753 kb on + strand at 2343.754 kb on - strand at 2343.754 kb on - strand at 2343.754 kb on - strand at 2343.768 kb on + strand at 2343.769 kb on - strand at 2343.769 kb on - strand at 2343.836 kb on + strand at 2343.839 kb on + strand at 2343.840 kb on - strand at 2343.840 kb on - strand at 2343.840 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,341,722 + Psyr_2017 0.59 +0.8 2,341,723 - Psyr_2017 0.59 +1.8 2,341,723 - Psyr_2017 0.59 -0.5 2,341,723 - Psyr_2017 0.59 -0.4 2,341,903 - Psyr_2017 0.69 -0.8 2,341,905 + Psyr_2017 0.69 -1.3 2,341,906 - Psyr_2017 0.69 -1.3 2,341,924 - Psyr_2017 0.70 -1.4 2,342,023 - Psyr_2017 0.75 -0.8 2,342,312 + +0.8 2,342,313 - +1.1 2,342,313 - +0.6 2,342,454 + -0.8 2,342,483 - +1.3 2,342,483 - -0.4 2,342,643 + +0.3 2,342,644 - +1.1 2,342,644 - +0.1 2,342,644 - +1.1 2,342,660 - +0.9 2,342,661 + -1.1 2,342,662 - Psyr_2018 0.10 -1.3 2,342,662 - Psyr_2018 0.10 -2.0 2,342,662 - Psyr_2018 0.10 -1.4 2,342,794 - Psyr_2018 0.28 -1.1 2,342,816 + Psyr_2018 0.31 -0.6 2,342,935 + Psyr_2018 0.47 -0.3 2,342,935 + Psyr_2018 0.47 -0.6 2,342,935 + Psyr_2018 0.47 -0.3 2,342,936 - Psyr_2018 0.48 -0.9 2,342,936 - Psyr_2018 0.48 -0.2 2,343,181 + Psyr_2018 0.81 +0.8 2,343,181 + Psyr_2018 0.81 -0.9 2,343,181 + Psyr_2018 0.81 -1.2 2,343,181 + Psyr_2018 0.81 +0.0 2,343,181 + Psyr_2018 0.81 +1.4 2,343,182 - Psyr_2018 0.81 -1.7 2,343,182 - Psyr_2018 0.81 +1.1 2,343,182 - Psyr_2018 0.81 +1.1 2,343,182 - Psyr_2018 0.81 -1.5 2,343,182 - Psyr_2018 0.81 -1.8 2,343,183 + Psyr_2018 0.81 -0.8 2,343,183 + Psyr_2018 0.81 +0.9 2,343,184 - Psyr_2018 0.82 -0.2 2,343,184 - Psyr_2018 0.82 -0.6 2,343,184 - Psyr_2018 0.82 -0.5 2,343,189 + Psyr_2018 0.82 -0.6 2,343,190 - Psyr_2018 0.82 -0.9 2,343,249 + -0.1 2,343,249 + +0.5 2,343,249 + +0.9 2,343,249 + -3.0 2,343,249 + +0.9 2,343,249 + -0.4 2,343,249 + -1.2 2,343,249 + -2.5 2,343,249 + -0.1 2,343,250 - +0.3 2,343,250 - +0.5 2,343,250 - +0.7 2,343,250 - -0.6 2,343,750 - +0.1 2,343,750 - +0.1 2,343,750 - +1.1 2,343,753 + +0.4 2,343,753 + -0.4 2,343,753 + -0.2 2,343,753 + +0.5 2,343,754 - -0.4 2,343,754 - -0.9 2,343,754 - +0.8 2,343,768 + +0.1 2,343,769 - +0.4 2,343,769 - -0.3 2,343,836 + -2.6 2,343,839 + -1.3 2,343,840 - -0.7 2,343,840 - -0.2 2,343,840 - -1.9
Or see this region's nucleotide sequence