Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1945 and Psyr_1946 are separated by 164 nucleotides Psyr_1946 and Psyr_1947 are separated by 81 nucleotides
Psyr_1945: Psyr_1945 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,217,771 to 2,230,781
_1945
Psyr_1946: Psyr_1946 - diaminobutyrate aminotransferase apoenzyme, at 2,230,946 to 2,232,358
_1946
Psyr_1947: Psyr_1947 - MbtH-like protein, at 2,232,440 to 2,232,658
_1947
Position (kb)
2230
2231
2232
2233 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2230.017 kb on + strand at 2230.018 kb on - strand at 2230.137 kb on + strand at 2230.138 kb on - strand at 2230.138 kb on - strand at 2230.138 kb on - strand at 2230.368 kb on + strand at 2230.369 kb on - strand at 2230.369 kb on - strand at 2230.640 kb on + strand at 2230.904 kb on + strand at 2230.904 kb on + strand at 2230.905 kb on - strand at 2231.015 kb on + strand at 2231.254 kb on + strand, within Psyr_1946 at 2231.255 kb on - strand, within Psyr_1946 at 2231.994 kb on - strand, within Psyr_1946 at 2232.010 kb on + strand, within Psyr_1946 at 2232.261 kb on + strand at 2232.262 kb on - strand at 2232.369 kb on + strand at 2232.369 kb on + strand at 2232.370 kb on - strand at 2232.375 kb on + strand at 2232.375 kb on + strand at 2232.391 kb on - strand at 2232.391 kb on - strand at 2232.391 kb on - strand at 2232.503 kb on + strand, within Psyr_1947 at 2232.504 kb on - strand, within Psyr_1947 at 2232.522 kb on - strand, within Psyr_1947 at 2232.522 kb on - strand, within Psyr_1947 at 2232.587 kb on + strand, within Psyr_1947 at 2232.801 kb on - strand at 2232.817 kb on - strand at 2232.983 kb on - strand at 2233.059 kb on + strand at 2233.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,230,017 + +0.5 2,230,018 - +1.3 2,230,137 + +0.7 2,230,138 - -0.3 2,230,138 - +1.0 2,230,138 - +0.2 2,230,368 + +1.0 2,230,369 - -1.3 2,230,369 - -0.9 2,230,640 + +0.0 2,230,904 + -1.7 2,230,904 + -0.3 2,230,905 - -0.5 2,231,015 + -0.2 2,231,254 + Psyr_1946 0.22 +0.2 2,231,255 - Psyr_1946 0.22 -2.1 2,231,994 - Psyr_1946 0.74 +2.6 2,232,010 + Psyr_1946 0.75 -1.3 2,232,261 + -0.4 2,232,262 - -0.7 2,232,369 + -0.1 2,232,369 + -0.1 2,232,370 - +0.7 2,232,375 + -1.0 2,232,375 + +0.7 2,232,391 - +0.2 2,232,391 - -2.1 2,232,391 - -0.4 2,232,503 + Psyr_1947 0.29 +0.6 2,232,504 - Psyr_1947 0.29 -0.4 2,232,522 - Psyr_1947 0.37 -0.3 2,232,522 - Psyr_1947 0.37 -1.2 2,232,587 + Psyr_1947 0.67 +0.2 2,232,801 - -0.9 2,232,817 - -0.1 2,232,983 - +0.6 2,233,059 + -0.8 2,233,060 - +0.5
Or see this region's nucleotide sequence