Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1942 and Psyr_1943 are separated by 78 nucleotides Psyr_1943 and Psyr_1944 are separated by 316 nucleotides Psyr_1944 and Psyr_1945 are separated by 95 nucleotides
Psyr_1942: Psyr_1942 - conserved hypothetical protein, at 2,215,461 to 2,215,985
_1942
Psyr_1943: Psyr_1943 - Sigma-70 region 2, at 2,216,064 to 2,216,600
_1943
Psyr_1944: Psyr_1944 - Thioesterase, at 2,216,917 to 2,217,675
_1944
Psyr_1945: Psyr_1945 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,217,771 to 2,230,781
_1945
Position (kb)
2216
2217
2218 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2216.195 kb on - strand, within Psyr_1943 at 2216.338 kb on + strand, within Psyr_1943 at 2216.338 kb on + strand, within Psyr_1943 at 2216.338 kb on + strand, within Psyr_1943 at 2216.339 kb on - strand, within Psyr_1943 at 2216.339 kb on - strand, within Psyr_1943 at 2216.404 kb on + strand, within Psyr_1943 at 2216.405 kb on - strand, within Psyr_1943 at 2216.405 kb on - strand, within Psyr_1943 at 2216.536 kb on + strand, within Psyr_1943 at 2216.536 kb on + strand, within Psyr_1943 at 2216.536 kb on + strand, within Psyr_1943 at 2216.536 kb on + strand, within Psyr_1943 at 2216.536 kb on + strand, within Psyr_1943 at 2216.537 kb on - strand, within Psyr_1943 at 2216.537 kb on - strand, within Psyr_1943 at 2216.537 kb on - strand, within Psyr_1943 at 2216.537 kb on - strand, within Psyr_1943 at 2216.576 kb on + strand at 2216.577 kb on - strand at 2216.577 kb on - strand at 2216.577 kb on - strand at 2216.586 kb on + strand at 2216.587 kb on - strand at 2216.620 kb on + strand at 2216.727 kb on + strand at 2216.773 kb on + strand at 2216.774 kb on - strand at 2216.777 kb on + strand at 2216.778 kb on - strand at 2216.838 kb on + strand at 2216.839 kb on - strand at 2216.885 kb on + strand at 2216.886 kb on - strand at 2216.886 kb on - strand at 2216.956 kb on + strand at 2216.980 kb on + strand at 2216.980 kb on + strand at 2216.980 kb on + strand at 2216.981 kb on - strand at 2216.981 kb on - strand at 2216.981 kb on - strand at 2217.137 kb on - strand, within Psyr_1944 at 2217.421 kb on + strand, within Psyr_1944 at 2217.458 kb on + strand, within Psyr_1944 at 2217.459 kb on - strand, within Psyr_1944 at 2217.459 kb on - strand, within Psyr_1944 at 2217.459 kb on - strand, within Psyr_1944 at 2217.563 kb on - strand, within Psyr_1944 at 2217.737 kb on + strand at 2217.738 kb on - strand at 2217.997 kb on - strand at 2217.997 kb on - strand at 2218.023 kb on + strand at 2218.024 kb on - strand at 2218.024 kb on - strand at 2218.284 kb on + strand at 2218.285 kb on - strand at 2218.285 kb on - strand at 2218.422 kb on + strand at 2218.423 kb on - strand at 2218.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,216,195 - Psyr_1943 0.24 -0.6 2,216,338 + Psyr_1943 0.51 +0.0 2,216,338 + Psyr_1943 0.51 -2.4 2,216,338 + Psyr_1943 0.51 +1.0 2,216,339 - Psyr_1943 0.51 -1.3 2,216,339 - Psyr_1943 0.51 -0.9 2,216,404 + Psyr_1943 0.63 -0.8 2,216,405 - Psyr_1943 0.64 +0.1 2,216,405 - Psyr_1943 0.64 -1.8 2,216,536 + Psyr_1943 0.88 -0.7 2,216,536 + Psyr_1943 0.88 -1.1 2,216,536 + Psyr_1943 0.88 -0.8 2,216,536 + Psyr_1943 0.88 +0.8 2,216,536 + Psyr_1943 0.88 -2.4 2,216,537 - Psyr_1943 0.88 +1.4 2,216,537 - Psyr_1943 0.88 +0.4 2,216,537 - Psyr_1943 0.88 -0.1 2,216,537 - Psyr_1943 0.88 +1.0 2,216,576 + -0.7 2,216,577 - +1.2 2,216,577 - -0.3 2,216,577 - -2.0 2,216,586 + -0.1 2,216,587 - +1.3 2,216,620 + -1.8 2,216,727 + -1.2 2,216,773 + +0.8 2,216,774 - -0.1 2,216,777 + +0.4 2,216,778 - +0.0 2,216,838 + -0.3 2,216,839 - +0.1 2,216,885 + -0.1 2,216,886 - +0.1 2,216,886 - -1.5 2,216,956 + -0.2 2,216,980 + -0.6 2,216,980 + +0.5 2,216,980 + +1.0 2,216,981 - -2.2 2,216,981 - +0.4 2,216,981 - +0.0 2,217,137 - Psyr_1944 0.29 +0.5 2,217,421 + Psyr_1944 0.66 -2.8 2,217,458 + Psyr_1944 0.71 -1.6 2,217,459 - Psyr_1944 0.71 -0.1 2,217,459 - Psyr_1944 0.71 -2.4 2,217,459 - Psyr_1944 0.71 -2.1 2,217,563 - Psyr_1944 0.85 +0.0 2,217,737 + +0.8 2,217,738 - -0.4 2,217,997 - +1.0 2,217,997 - +1.4 2,218,023 + -0.7 2,218,024 - +0.4 2,218,024 - -0.4 2,218,284 + +0.4 2,218,285 - -0.6 2,218,285 - +0.4 2,218,422 + -3.0 2,218,423 - +0.8 2,218,501 - -2.9
Or see this region's nucleotide sequence