Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1894 and Psyr_1895 are separated by 75 nucleotides Psyr_1895 and Psyr_1896 are separated by 11 nucleotides Psyr_1896 and Psyr_1897 are separated by 190 nucleotides
Psyr_1894: Psyr_1894 - SNF2-related:Helicase, C-terminal:SWIM Zn-finger, at 2,163,618 to 2,166,362
_1894
Psyr_1895: Psyr_1895 - conserved hypothetical protein, at 2,166,438 to 2,166,962
_1895
Psyr_1896: Psyr_1896 - transcription-repair coupling factor, at 2,166,974 to 2,170,426
_1896
Psyr_1897: Psyr_1897 - Glyceraldehyde-3-phosphate dehydrogenase, type I, at 2,170,617 to 2,172,080
_1897
Position (kb)
2166
2167
2168
2169
2170
2171 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2166.151 kb on + strand at 2166.151 kb on + strand at 2166.151 kb on + strand at 2166.151 kb on + strand at 2166.151 kb on + strand at 2166.151 kb on + strand at 2166.152 kb on - strand at 2166.152 kb on - strand at 2166.152 kb on - strand at 2166.152 kb on - strand at 2166.197 kb on - strand at 2166.280 kb on + strand at 2166.281 kb on - strand at 2166.315 kb on + strand at 2166.315 kb on + strand at 2166.428 kb on + strand at 2166.428 kb on + strand at 2166.429 kb on - strand at 2166.429 kb on - strand at 2166.432 kb on + strand at 2166.432 kb on + strand at 2166.432 kb on + strand at 2166.433 kb on - strand at 2166.433 kb on - strand at 2166.433 kb on - strand at 2166.433 kb on - strand at 2166.433 kb on - strand at 2166.487 kb on + strand at 2166.487 kb on + strand at 2166.487 kb on + strand at 2166.487 kb on + strand at 2166.488 kb on - strand at 2166.488 kb on - strand at 2166.488 kb on - strand at 2166.507 kb on + strand, within Psyr_1895 at 2166.731 kb on - strand, within Psyr_1895 at 2166.731 kb on - strand, within Psyr_1895 at 2166.731 kb on - strand, within Psyr_1895 at 2167.232 kb on - strand at 2167.338 kb on + strand, within Psyr_1896 at 2167.338 kb on - strand, within Psyr_1896 at 2167.339 kb on - strand, within Psyr_1896 at 2167.339 kb on - strand, within Psyr_1896 at 2167.371 kb on + strand, within Psyr_1896 at 2167.494 kb on + strand, within Psyr_1896 at 2167.494 kb on + strand, within Psyr_1896 at 2167.495 kb on - strand, within Psyr_1896 at 2167.613 kb on - strand, within Psyr_1896 at 2167.680 kb on + strand, within Psyr_1896 at 2167.686 kb on + strand, within Psyr_1896 at 2167.845 kb on + strand, within Psyr_1896 at 2167.977 kb on + strand, within Psyr_1896 at 2167.977 kb on + strand, within Psyr_1896 at 2167.977 kb on + strand, within Psyr_1896 at 2167.978 kb on - strand, within Psyr_1896 at 2167.978 kb on - strand, within Psyr_1896 at 2167.980 kb on + strand, within Psyr_1896 at 2167.980 kb on + strand, within Psyr_1896 at 2167.980 kb on + strand, within Psyr_1896 at 2167.981 kb on - strand, within Psyr_1896 at 2168.057 kb on - strand, within Psyr_1896 at 2168.071 kb on + strand, within Psyr_1896 at 2168.167 kb on + strand, within Psyr_1896 at 2168.421 kb on + strand, within Psyr_1896 at 2168.421 kb on + strand, within Psyr_1896 at 2168.422 kb on - strand, within Psyr_1896 at 2168.422 kb on - strand, within Psyr_1896 at 2168.422 kb on - strand, within Psyr_1896 at 2168.677 kb on - strand, within Psyr_1896 at 2168.703 kb on + strand, within Psyr_1896 at 2168.703 kb on + strand, within Psyr_1896 at 2168.703 kb on + strand, within Psyr_1896 at 2168.704 kb on - strand, within Psyr_1896 at 2168.704 kb on - strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.859 kb on + strand, within Psyr_1896 at 2168.860 kb on - strand, within Psyr_1896 at 2168.860 kb on - strand, within Psyr_1896 at 2168.860 kb on - strand, within Psyr_1896 at 2168.860 kb on - strand, within Psyr_1896 at 2168.941 kb on - strand, within Psyr_1896 at 2169.465 kb on - strand, within Psyr_1896 at 2169.606 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.648 kb on + strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.649 kb on - strand, within Psyr_1896 at 2169.651 kb on + strand, within Psyr_1896 at 2169.652 kb on - strand, within Psyr_1896 at 2169.652 kb on - strand, within Psyr_1896 at 2169.674 kb on - strand, within Psyr_1896 at 2169.674 kb on - strand, within Psyr_1896 at 2169.957 kb on + strand, within Psyr_1896 at 2169.957 kb on + strand, within Psyr_1896 at 2170.237 kb on + strand at 2170.237 kb on + strand at 2170.237 kb on + strand at 2170.237 kb on + strand at 2170.237 kb on + strand at 2170.238 kb on - strand at 2170.238 kb on - strand at 2170.505 kb on + strand at 2170.506 kb on - strand at 2170.508 kb on + strand at 2170.515 kb on + strand at 2170.516 kb on - strand at 2170.536 kb on + strand at 2170.536 kb on + strand at 2170.536 kb on + strand at 2170.537 kb on - strand at 2170.537 kb on - strand at 2170.537 kb on - strand at 2170.549 kb on + strand at 2170.589 kb on + strand at 2170.589 kb on + strand at 2170.589 kb on + strand at 2170.594 kb on + strand at 2170.594 kb on + strand at 2170.614 kb on + strand at 2170.614 kb on + strand at 2170.731 kb on - strand at 2170.731 kb on - strand at 2170.749 kb on + strand at 2170.750 kb on - strand at 2170.750 kb on - strand at 2170.844 kb on - strand at 2171.214 kb on - strand, within Psyr_1897 at 2171.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 2,166,151 + +1.4 2,166,151 + +0.2 2,166,151 + -0.7 2,166,151 + +0.2 2,166,151 + +0.3 2,166,151 + +0.3 2,166,152 - +0.7 2,166,152 - +0.7 2,166,152 - +0.2 2,166,152 - -0.3 2,166,197 - -0.8 2,166,280 + +0.0 2,166,281 - -0.2 2,166,315 + +0.4 2,166,315 + -1.0 2,166,428 + -0.6 2,166,428 + -0.9 2,166,429 - -0.8 2,166,429 - +0.2 2,166,432 + -0.6 2,166,432 + -1.3 2,166,432 + +0.5 2,166,433 - -0.8 2,166,433 - -0.4 2,166,433 - -0.6 2,166,433 - -0.3 2,166,433 - +0.4 2,166,487 + +0.6 2,166,487 + -0.2 2,166,487 + +0.4 2,166,487 + +0.3 2,166,488 - +3.0 2,166,488 - -0.7 2,166,488 - +0.7 2,166,507 + Psyr_1895 0.13 +0.6 2,166,731 - Psyr_1895 0.56 +0.8 2,166,731 - Psyr_1895 0.56 +0.7 2,166,731 - Psyr_1895 0.56 +0.4 2,167,232 - +1.0 2,167,338 + Psyr_1896 0.11 -0.6 2,167,338 - Psyr_1896 0.11 +3.3 2,167,339 - Psyr_1896 0.11 +1.2 2,167,339 - Psyr_1896 0.11 -1.9 2,167,371 + Psyr_1896 0.11 +0.0 2,167,494 + Psyr_1896 0.15 +4.0 2,167,494 + Psyr_1896 0.15 -1.6 2,167,495 - Psyr_1896 0.15 -0.4 2,167,613 - Psyr_1896 0.19 -1.3 2,167,680 + Psyr_1896 0.20 +0.9 2,167,686 + Psyr_1896 0.21 +0.7 2,167,845 + Psyr_1896 0.25 +3.0 2,167,977 + Psyr_1896 0.29 -0.1 2,167,977 + Psyr_1896 0.29 +0.4 2,167,977 + Psyr_1896 0.29 +1.6 2,167,978 - Psyr_1896 0.29 +0.3 2,167,978 - Psyr_1896 0.29 +1.1 2,167,980 + Psyr_1896 0.29 -0.6 2,167,980 + Psyr_1896 0.29 +1.8 2,167,980 + Psyr_1896 0.29 -0.5 2,167,981 - Psyr_1896 0.29 -0.3 2,168,057 - Psyr_1896 0.31 -0.1 2,168,071 + Psyr_1896 0.32 +1.0 2,168,167 + Psyr_1896 0.35 +0.7 2,168,421 + Psyr_1896 0.42 +0.0 2,168,421 + Psyr_1896 0.42 +2.0 2,168,422 - Psyr_1896 0.42 +2.0 2,168,422 - Psyr_1896 0.42 -0.6 2,168,422 - Psyr_1896 0.42 +1.2 2,168,677 - Psyr_1896 0.49 +0.7 2,168,703 + Psyr_1896 0.50 +0.1 2,168,703 + Psyr_1896 0.50 +1.5 2,168,703 + Psyr_1896 0.50 -0.6 2,168,704 - Psyr_1896 0.50 +0.9 2,168,704 - Psyr_1896 0.50 +1.0 2,168,859 + Psyr_1896 0.55 -1.3 2,168,859 + Psyr_1896 0.55 -0.4 2,168,859 + Psyr_1896 0.55 +2.2 2,168,859 + Psyr_1896 0.55 +1.4 2,168,859 + Psyr_1896 0.55 +0.4 2,168,859 + Psyr_1896 0.55 +2.2 2,168,860 - Psyr_1896 0.55 +0.5 2,168,860 - Psyr_1896 0.55 -0.6 2,168,860 - Psyr_1896 0.55 +0.0 2,168,860 - Psyr_1896 0.55 +0.0 2,168,941 - Psyr_1896 0.57 +1.0 2,169,465 - Psyr_1896 0.72 +1.6 2,169,606 + Psyr_1896 0.76 +0.1 2,169,648 + Psyr_1896 0.77 -0.6 2,169,648 + Psyr_1896 0.77 +3.0 2,169,648 + Psyr_1896 0.77 +0.0 2,169,648 + Psyr_1896 0.77 +0.1 2,169,648 + Psyr_1896 0.77 +0.0 2,169,648 + Psyr_1896 0.77 -0.4 2,169,648 + Psyr_1896 0.77 +0.4 2,169,649 - Psyr_1896 0.77 -1.4 2,169,649 - Psyr_1896 0.77 +0.0 2,169,649 - Psyr_1896 0.77 -0.7 2,169,649 - Psyr_1896 0.77 -2.1 2,169,649 - Psyr_1896 0.77 +0.0 2,169,649 - Psyr_1896 0.77 -0.4 2,169,649 - Psyr_1896 0.77 +1.0 2,169,649 - Psyr_1896 0.77 -1.7 2,169,649 - Psyr_1896 0.77 -1.0 2,169,651 + Psyr_1896 0.78 +0.3 2,169,652 - Psyr_1896 0.78 +0.8 2,169,652 - Psyr_1896 0.78 +0.6 2,169,674 - Psyr_1896 0.78 -1.3 2,169,674 - Psyr_1896 0.78 -1.6 2,169,957 + Psyr_1896 0.86 -1.8 2,169,957 + Psyr_1896 0.86 +0.7 2,170,237 + -0.9 2,170,237 + -2.3 2,170,237 + +0.4 2,170,237 + +0.2 2,170,237 + +0.6 2,170,238 - +0.2 2,170,238 - +1.2 2,170,505 + +1.2 2,170,506 - -1.6 2,170,508 + +0.4 2,170,515 + +0.2 2,170,516 - -0.1 2,170,536 + +0.4 2,170,536 + -2.3 2,170,536 + +0.0 2,170,537 - -2.1 2,170,537 - +0.4 2,170,537 - +2.6 2,170,549 + -0.0 2,170,589 + +0.0 2,170,589 + -0.5 2,170,589 + +0.7 2,170,594 + -0.4 2,170,594 + +2.0 2,170,614 + +1.6 2,170,614 + -1.2 2,170,731 - +1.0 2,170,731 - +0.0 2,170,749 + +0.7 2,170,750 - +2.0 2,170,750 - +0.8 2,170,844 - +2.0 2,171,214 - Psyr_1897 0.41 -1.0 2,171,274 + +2.0
Or see this region's nucleotide sequence