Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1258 and Psyr_1259 are separated by 7 nucleotides Psyr_1259 and Psyr_1260 are separated by 119 nucleotides Psyr_1260 and Psyr_1261 are separated by 94 nucleotides
Psyr_1258: Psyr_1258 - Peptidase M23B, at 1,415,130 to 1,415,954
_1258
Psyr_1259: Psyr_1259 - Exodeoxyribonuclease VII large subunit, at 1,415,962 to 1,417,353
_1259
Psyr_1260: Psyr_1260 - conserved hypothetical protein, at 1,417,473 to 1,418,021
_1260
Psyr_1261: Psyr_1261 - inosine-5'-monophosphate dehydrogenase, at 1,418,116 to 1,419,585
_1261
Position (kb)
1415
1416
1417
1418 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1415.024 kb on - strand at 1415.055 kb on - strand at 1415.131 kb on + strand at 1415.131 kb on + strand at 1415.132 kb on - strand at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.337 kb on + strand, within Psyr_1258 at 1415.338 kb on - strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.889 kb on - strand at 1415.949 kb on + strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259 at 1417.040 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.181 kb on + strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.182 kb on - strand, within Psyr_1259 at 1417.293 kb on + strand at 1417.294 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.354 kb on - strand at 1417.434 kb on + strand at 1417.458 kb on - strand at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.605 kb on + strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.606 kb on - strand, within Psyr_1260 at 1417.607 kb on + strand, within Psyr_1260 at 1417.608 kb on - strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.722 kb on + strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.723 kb on - strand, within Psyr_1260 at 1417.928 kb on + strand, within Psyr_1260 at 1418.017 kb on + strand at 1418.017 kb on + strand at 1418.019 kb on + strand at 1418.020 kb on - strand at 1418.035 kb on + strand at 1418.036 kb on - strand at 1418.047 kb on + strand at 1418.047 kb on + strand at 1418.047 kb on + strand at 1418.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 1,415,024 - +0.7 1,415,055 - +0.5 1,415,131 + -2.0 1,415,131 + -0.2 1,415,132 - -0.8 1,415,294 + Psyr_1258 0.20 -0.3 1,415,294 + Psyr_1258 0.20 +0.1 1,415,294 + Psyr_1258 0.20 -0.2 1,415,294 + Psyr_1258 0.20 +0.3 1,415,294 + Psyr_1258 0.20 +0.6 1,415,294 + Psyr_1258 0.20 +0.9 1,415,295 - Psyr_1258 0.20 +2.1 1,415,295 - Psyr_1258 0.20 -0.1 1,415,295 - Psyr_1258 0.20 -1.0 1,415,295 - Psyr_1258 0.20 +0.1 1,415,295 - Psyr_1258 0.20 -0.0 1,415,295 - Psyr_1258 0.20 +0.7 1,415,295 - Psyr_1258 0.20 +2.1 1,415,337 + Psyr_1258 0.25 +0.2 1,415,338 - Psyr_1258 0.25 -0.5 1,415,359 + Psyr_1258 0.28 +0.2 1,415,359 + Psyr_1258 0.28 +0.4 1,415,359 + Psyr_1258 0.28 -0.4 1,415,359 + Psyr_1258 0.28 +0.2 1,415,359 + Psyr_1258 0.28 +1.9 1,415,359 + Psyr_1258 0.28 +0.8 1,415,359 + Psyr_1258 0.28 -1.2 1,415,359 + Psyr_1258 0.28 -0.3 1,415,359 + Psyr_1258 0.28 -0.2 1,415,360 - Psyr_1258 0.28 -0.8 1,415,360 - Psyr_1258 0.28 -0.5 1,415,360 - Psyr_1258 0.28 -0.7 1,415,803 + Psyr_1258 0.82 -0.6 1,415,803 + Psyr_1258 0.82 +0.1 1,415,803 + Psyr_1258 0.82 +0.7 1,415,803 + Psyr_1258 0.82 -1.0 1,415,804 - Psyr_1258 0.82 +1.1 1,415,804 - Psyr_1258 0.82 +1.1 1,415,804 - Psyr_1258 0.82 +4.3 1,415,804 - Psyr_1258 0.82 +0.5 1,415,804 - Psyr_1258 0.82 +2.4 1,415,888 + +3.7 1,415,888 + -1.5 1,415,888 + +0.1 1,415,888 + -0.2 1,415,888 + +0.8 1,415,889 - +2.1 1,415,949 + +0.6 1,415,950 - -0.5 1,415,950 - +0.8 1,415,950 - +0.5 1,415,950 - +1.3 1,416,175 + Psyr_1259 0.15 +4.3 1,416,175 + Psyr_1259 0.15 -0.3 1,416,175 + Psyr_1259 0.15 +1.7 1,416,176 - Psyr_1259 0.15 +0.2 1,416,176 - Psyr_1259 0.15 +0.2 1,417,040 + Psyr_1259 0.77 +0.7 1,417,181 + Psyr_1259 0.88 -0.4 1,417,181 + Psyr_1259 0.88 +0.1 1,417,181 + Psyr_1259 0.88 +0.1 1,417,181 + Psyr_1259 0.88 -0.5 1,417,181 + Psyr_1259 0.88 +0.8 1,417,181 + Psyr_1259 0.88 -1.0 1,417,181 + Psyr_1259 0.88 +1.0 1,417,181 + Psyr_1259 0.88 +1.1 1,417,182 - Psyr_1259 0.88 +1.4 1,417,182 - Psyr_1259 0.88 +0.6 1,417,182 - Psyr_1259 0.88 +0.1 1,417,182 - Psyr_1259 0.88 +0.2 1,417,182 - Psyr_1259 0.88 +0.1 1,417,182 - Psyr_1259 0.88 +0.1 1,417,182 - Psyr_1259 0.88 -0.3 1,417,182 - Psyr_1259 0.88 +0.5 1,417,182 - Psyr_1259 0.88 -1.1 1,417,182 - Psyr_1259 0.88 +0.5 1,417,293 + -0.9 1,417,294 - +0.2 1,417,354 - -1.7 1,417,354 - +1.5 1,417,354 - -2.0 1,417,354 - -0.2 1,417,434 + -0.7 1,417,458 - +1.4 1,417,605 + Psyr_1260 0.24 +0.1 1,417,605 + Psyr_1260 0.24 +1.3 1,417,605 + Psyr_1260 0.24 +0.9 1,417,605 + Psyr_1260 0.24 +0.5 1,417,605 + Psyr_1260 0.24 +1.5 1,417,605 + Psyr_1260 0.24 -0.4 1,417,605 + Psyr_1260 0.24 +0.1 1,417,606 - Psyr_1260 0.24 +0.3 1,417,606 - Psyr_1260 0.24 +0.8 1,417,606 - Psyr_1260 0.24 -0.5 1,417,606 - Psyr_1260 0.24 +1.1 1,417,606 - Psyr_1260 0.24 -0.1 1,417,606 - Psyr_1260 0.24 -1.3 1,417,607 + Psyr_1260 0.24 -0.7 1,417,608 - Psyr_1260 0.25 +0.6 1,417,722 + Psyr_1260 0.45 +0.1 1,417,722 + Psyr_1260 0.45 +0.9 1,417,722 + Psyr_1260 0.45 +0.7 1,417,722 + Psyr_1260 0.45 +0.3 1,417,722 + Psyr_1260 0.45 -0.8 1,417,722 + Psyr_1260 0.45 -1.3 1,417,722 + Psyr_1260 0.45 -0.6 1,417,722 + Psyr_1260 0.45 -0.1 1,417,722 + Psyr_1260 0.45 -0.1 1,417,722 + Psyr_1260 0.45 -0.5 1,417,722 + Psyr_1260 0.45 -1.9 1,417,722 + Psyr_1260 0.45 +1.0 1,417,722 + Psyr_1260 0.45 +2.5 1,417,722 + Psyr_1260 0.45 +1.5 1,417,722 + Psyr_1260 0.45 -1.6 1,417,723 - Psyr_1260 0.46 +0.3 1,417,723 - Psyr_1260 0.46 +0.1 1,417,723 - Psyr_1260 0.46 -1.1 1,417,723 - Psyr_1260 0.46 -1.7 1,417,723 - Psyr_1260 0.46 -2.8 1,417,723 - Psyr_1260 0.46 -0.5 1,417,723 - Psyr_1260 0.46 -0.9 1,417,723 - Psyr_1260 0.46 -1.3 1,417,723 - Psyr_1260 0.46 +0.5 1,417,723 - Psyr_1260 0.46 -0.1 1,417,723 - Psyr_1260 0.46 +0.6 1,417,723 - Psyr_1260 0.46 -0.4 1,417,723 - Psyr_1260 0.46 +0.5 1,417,723 - Psyr_1260 0.46 +1.7 1,417,723 - Psyr_1260 0.46 +0.3 1,417,723 - Psyr_1260 0.46 -0.6 1,417,928 + Psyr_1260 0.83 -0.3 1,418,017 + -1.2 1,418,017 + -0.5 1,418,019 + -0.9 1,418,020 - -1.7 1,418,035 + +1.7 1,418,036 - -0.9 1,418,047 + -0.5 1,418,047 + +1.5 1,418,047 + -1.5 1,418,047 + -2.2
Or see this region's nucleotide sequence