Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0456 and Psyr_0457 are separated by 27 nucleotides Psyr_0457 and Psyr_0458 are separated by 393 nucleotides Psyr_0458 and Psyr_0459 are separated by 311 nucleotides
Psyr_0456: Psyr_0456 - Cytochrome B561, bacterial, at 493,396 to 493,944
_0456
Psyr_0457: Psyr_0457 - YceI, at 493,972 to 494,550
_0457
Psyr_0458: Psyr_0458 - Helicase, C-terminal:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase, N-terminal, at 494,944 to 496,833
_0458
Psyr_0459: Psyr_0459 - 5,10-methylenetetrahydrofolate reductase, at 497,145 to 497,990
_0459
Position (kb) 494
495
496
497 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 493.965 kb on + strand at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.047 kb on + strand, within Psyr_0457 at 494.048 kb on - strand, within Psyr_0457 at 494.108 kb on - strand, within Psyr_0457 at 494.108 kb on - strand, within Psyr_0457 at 494.124 kb on + strand, within Psyr_0457 at 494.124 kb on + strand, within Psyr_0457 at 494.125 kb on - strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.278 kb on + strand, within Psyr_0457 at 494.366 kb on + strand, within Psyr_0457 at 494.537 kb on + strand at 494.538 kb on - strand at 494.538 kb on - strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.548 kb on + strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.549 kb on - strand at 494.665 kb on + strand at 494.665 kb on + strand at 494.666 kb on - strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.667 kb on + strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.668 kb on - strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.752 kb on + strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.753 kb on - strand at 494.754 kb on + strand at 494.808 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.839 kb on + strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.840 kb on - strand at 494.843 kb on + strand at 494.843 kb on + strand at 494.843 kb on + strand at 494.844 kb on - strand at 494.844 kb on - strand at 494.844 kb on - strand at 494.889 kb on - strand at 494.889 kb on - strand at 495.362 kb on + strand, within Psyr_0458 at 495.362 kb on + strand, within Psyr_0458 at 495.363 kb on - strand, within Psyr_0458 at 495.388 kb on + strand, within Psyr_0458 at 495.388 kb on + strand, within Psyr_0458 at 495.389 kb on - strand, within Psyr_0458 at 495.389 kb on - strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.836 kb on + strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.837 kb on - strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.934 kb on + strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 495.935 kb on - strand, within Psyr_0458 at 496.258 kb on - strand, within Psyr_0458 at 496.683 kb on + strand at 496.684 kb on - strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.897 kb on - strand at 497.042 kb on + strand at 497.042 kb on + strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.043 kb on - strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.044 kb on + strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.045 kb on - strand at 497.061 kb on + strand at 497.089 kb on + strand at 497.089 kb on + strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.207 kb on - strand at 497.207 kb on - strand at 497.306 kb on - strand, within Psyr_0459 at 497.322 kb on - strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.729 kb on - strand, within Psyr_0459 at 497.769 kb on + strand, within Psyr_0459
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 493,965 + -0.3 494,047 + Psyr_0457 0.13 +1.1 494,047 + Psyr_0457 0.13 +0.1 494,047 + Psyr_0457 0.13 +0.2 494,047 + Psyr_0457 0.13 +0.3 494,048 - Psyr_0457 0.13 -0.3 494,108 - Psyr_0457 0.23 -0.7 494,108 - Psyr_0457 0.23 -1.9 494,124 + Psyr_0457 0.26 -0.5 494,124 + Psyr_0457 0.26 -0.3 494,125 - Psyr_0457 0.26 -0.7 494,278 + Psyr_0457 0.53 -0.6 494,278 + Psyr_0457 0.53 -0.9 494,278 + Psyr_0457 0.53 -0.4 494,366 + Psyr_0457 0.68 +0.0 494,537 + -1.3 494,538 - +0.0 494,538 - +1.3 494,548 + -0.6 494,548 + +0.4 494,548 + -0.3 494,548 + -0.2 494,548 + +0.7 494,549 - -0.8 494,549 - -0.1 494,549 - -0.0 494,549 - -0.5 494,549 - +0.2 494,549 - +1.2 494,665 + -0.4 494,665 + +0.5 494,666 - -0.2 494,667 + -0.6 494,667 + +0.1 494,667 + +2.0 494,667 + -1.1 494,667 + +1.5 494,667 + -0.4 494,667 + -0.7 494,667 + -0.4 494,667 + +0.6 494,667 + -0.4 494,667 + +1.5 494,667 + -0.9 494,667 + +0.5 494,667 + -0.7 494,667 + +0.6 494,667 + -0.4 494,667 + +0.4 494,667 + -0.6 494,667 + -0.4 494,667 + +0.3 494,667 + +0.2 494,667 + +1.3 494,667 + +0.3 494,668 - +2.8 494,668 - -0.9 494,668 - -0.1 494,668 - -0.8 494,668 - -0.6 494,668 - +0.7 494,668 - -1.0 494,668 - +0.6 494,668 - -0.2 494,668 - -0.5 494,668 - -0.2 494,668 - -1.2 494,668 - +0.6 494,668 - +2.2 494,668 - +1.6 494,668 - -3.3 494,668 - -0.0 494,752 + +0.3 494,752 + -1.6 494,752 + +0.5 494,752 + -0.5 494,752 + -0.8 494,752 + +0.6 494,752 + +0.9 494,753 - +0.5 494,753 - -1.0 494,753 - +0.2 494,753 - -0.8 494,753 - +0.4 494,754 + -0.8 494,808 + +0.3 494,839 + +0.5 494,839 + +0.2 494,839 + +0.8 494,839 + +0.3 494,839 + +0.3 494,839 + +0.4 494,840 - +0.0 494,840 - +0.5 494,840 - +1.2 494,840 - +0.5 494,840 - +1.2 494,840 - -0.2 494,840 - -1.0 494,843 + +0.5 494,843 + +0.4 494,843 + -0.5 494,844 - +0.9 494,844 - -0.4 494,844 - -1.3 494,889 - +0.7 494,889 - -1.3 495,362 + Psyr_0458 0.22 -1.4 495,362 + Psyr_0458 0.22 +1.6 495,363 - Psyr_0458 0.22 +0.7 495,388 + Psyr_0458 0.23 +0.1 495,388 + Psyr_0458 0.23 +1.5 495,389 - Psyr_0458 0.24 -0.6 495,389 - Psyr_0458 0.24 +0.2 495,836 + Psyr_0458 0.47 -0.3 495,836 + Psyr_0458 0.47 +0.4 495,836 + Psyr_0458 0.47 -1.0 495,836 + Psyr_0458 0.47 +0.4 495,836 + Psyr_0458 0.47 -2.0 495,837 - Psyr_0458 0.47 -1.0 495,837 - Psyr_0458 0.47 +0.9 495,837 - Psyr_0458 0.47 -0.3 495,837 - Psyr_0458 0.47 +0.2 495,837 - Psyr_0458 0.47 +0.9 495,837 - Psyr_0458 0.47 +0.2 495,934 + Psyr_0458 0.52 -1.7 495,934 + Psyr_0458 0.52 -1.4 495,934 + Psyr_0458 0.52 +0.6 495,934 + Psyr_0458 0.52 +0.9 495,934 + Psyr_0458 0.52 +1.4 495,934 + Psyr_0458 0.52 -2.1 495,934 + Psyr_0458 0.52 +0.8 495,934 + Psyr_0458 0.52 -0.3 495,935 - Psyr_0458 0.52 -1.2 495,935 - Psyr_0458 0.52 +0.7 495,935 - Psyr_0458 0.52 +0.3 495,935 - Psyr_0458 0.52 -1.1 495,935 - Psyr_0458 0.52 +0.1 495,935 - Psyr_0458 0.52 -0.2 495,935 - Psyr_0458 0.52 -0.5 495,935 - Psyr_0458 0.52 -0.9 495,935 - Psyr_0458 0.52 +1.5 496,258 - Psyr_0458 0.70 +1.4 496,683 + -0.8 496,684 - +0.6 496,837 + -2.5 496,837 + +0.8 496,837 + -1.0 496,858 + -0.4 496,858 + +0.5 496,858 + -0.0 496,858 + +0.2 496,897 - +1.1 497,042 + +0.1 497,042 + -0.9 497,043 - +0.2 497,043 - +0.1 497,043 - +0.7 497,044 + -0.2 497,044 + +0.3 497,044 + +2.0 497,045 - -0.8 497,045 - +0.1 497,045 - +0.7 497,061 + +0.4 497,089 + -0.4 497,089 + +0.8 497,090 - -0.0 497,090 - +0.3 497,090 - +2.0 497,207 - -0.3 497,207 - +0.1 497,306 - Psyr_0459 0.19 -1.3 497,322 - Psyr_0459 0.21 +0.6 497,380 + Psyr_0459 0.28 +0.2 497,380 + Psyr_0459 0.28 -0.3 497,380 + Psyr_0459 0.28 +1.1 497,380 + Psyr_0459 0.28 -0.1 497,380 + Psyr_0459 0.28 +1.4 497,381 - Psyr_0459 0.28 +0.4 497,381 - Psyr_0459 0.28 -1.6 497,381 - Psyr_0459 0.28 -0.4 497,381 - Psyr_0459 0.28 +0.4 497,381 - Psyr_0459 0.28 -0.1 497,458 + Psyr_0459 0.37 -0.2 497,458 + Psyr_0459 0.37 -0.1 497,458 + Psyr_0459 0.37 +1.3 497,458 + Psyr_0459 0.37 -1.6 497,458 + Psyr_0459 0.37 +0.4 497,458 + Psyr_0459 0.37 -1.4 497,458 + Psyr_0459 0.37 -0.3 497,458 + Psyr_0459 0.37 +0.2 497,458 + Psyr_0459 0.37 -0.2 497,459 - Psyr_0459 0.37 -0.0 497,459 - Psyr_0459 0.37 -1.4 497,459 - Psyr_0459 0.37 -1.0 497,479 + Psyr_0459 0.39 +2.2 497,479 + Psyr_0459 0.39 +1.0 497,479 + Psyr_0459 0.39 -0.1 497,480 - Psyr_0459 0.40 +1.6 497,480 - Psyr_0459 0.40 +1.2 497,728 + Psyr_0459 0.69 +0.3 497,729 - Psyr_0459 0.69 +0.4 497,729 - Psyr_0459 0.69 -0.1 497,769 + Psyr_0459 0.74 -0.5
Or see this region's nucleotide sequence