Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS17570

Experiment: Pimelic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS17560 and ABZR87_RS17565 overlap by 17 nucleotidesABZR87_RS17565 and ABZR87_RS17570 are separated by 95 nucleotidesABZR87_RS17570 and ABZR87_RS17575 are separated by 79 nucleotides ABZR87_RS17560: ABZR87_RS17560 - ABC transporter substrate-binding protein, at 301,651 to 302,859 _RS17560 ABZR87_RS17565: ABZR87_RS17565 - helix-turn-helix transcriptional regulator, at 302,843 to 303,646 _RS17565 ABZR87_RS17570: ABZR87_RS17570 - hypothetical protein, at 303,742 to 304,038 _RS17570 ABZR87_RS17575: ABZR87_RS17575 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 304,118 to 305,422 _RS17575 Position (kb) 303 304 305Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 302.771 kb on - strandat 302.773 kb on + strandat 302.773 kb on + strandat 302.774 kb on - strandat 302.774 kb on - strandat 302.814 kb on + strandat 302.815 kb on - strandat 303.128 kb on - strand, within ABZR87_RS17565at 303.145 kb on + strand, within ABZR87_RS17565at 303.146 kb on - strand, within ABZR87_RS17565at 303.378 kb on + strand, within ABZR87_RS17565at 303.379 kb on - strand, within ABZR87_RS17565at 303.426 kb on - strand, within ABZR87_RS17565at 303.585 kb on + strandat 303.586 kb on - strandat 303.586 kb on - strandat 303.586 kb on - strandat 303.586 kb on - strandat 303.586 kb on - strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.704 kb on + strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.705 kb on - strandat 303.935 kb on + strand, within ABZR87_RS17570at 303.936 kb on - strand, within ABZR87_RS17570at 304.081 kb on + strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.082 kb on - strandat 304.226 kb on + strandat 304.226 kb on + strandat 304.304 kb on + strand, within ABZR87_RS17575at 304.304 kb on + strand, within ABZR87_RS17575at 304.304 kb on + strand, within ABZR87_RS17575at 304.304 kb on + strand, within ABZR87_RS17575at 304.305 kb on - strand, within ABZR87_RS17575at 304.305 kb on - strand, within ABZR87_RS17575at 304.305 kb on - strand, within ABZR87_RS17575at 304.412 kb on + strand, within ABZR87_RS17575at 304.412 kb on + strand, within ABZR87_RS17575at 304.469 kb on + strand, within ABZR87_RS17575at 304.481 kb on + strand, within ABZR87_RS17575at 304.481 kb on + strand, within ABZR87_RS17575at 304.481 kb on + strand, within ABZR87_RS17575at 304.481 kb on + strand, within ABZR87_RS17575at 304.481 kb on + strand, within ABZR87_RS17575at 304.482 kb on - strand, within ABZR87_RS17575at 304.706 kb on + strand, within ABZR87_RS17575at 304.706 kb on + strand, within ABZR87_RS17575at 304.707 kb on - strand, within ABZR87_RS17575at 304.763 kb on + strand, within ABZR87_RS17575at 304.763 kb on + strand, within ABZR87_RS17575at 304.764 kb on - strand, within ABZR87_RS17575at 304.764 kb on - strand, within ABZR87_RS17575at 304.764 kb on - strand, within ABZR87_RS17575at 304.874 kb on + strand, within ABZR87_RS17575at 304.874 kb on + strand, within ABZR87_RS17575at 304.875 kb on - strand, within ABZR87_RS17575at 304.875 kb on - strand, within ABZR87_RS17575at 304.875 kb on - strand, within ABZR87_RS17575at 304.889 kb on + strand, within ABZR87_RS17575at 304.890 kb on - strand, within ABZR87_RS17575at 304.916 kb on + strand, within ABZR87_RS17575at 304.916 kb on + strand, within ABZR87_RS17575at 304.916 kb on + strand, within ABZR87_RS17575

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Per-strain Table

Position Strand Gene LocusTag Fraction Pimelic (C)
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302,771 - -2.6
302,773 + -4.0
302,773 + -1.5
302,774 - -1.4
302,774 - -4.5
302,814 + -2.7
302,815 - -2.4
303,128 - ABZR87_RS17565 0.35 -1.2
303,145 + ABZR87_RS17565 0.38 -1.1
303,146 - ABZR87_RS17565 0.38 +0.9
303,378 + ABZR87_RS17565 0.67 +0.1
303,379 - ABZR87_RS17565 0.67 -0.6
303,426 - ABZR87_RS17565 0.73 +0.1
303,585 + +0.0
303,586 - -0.2
303,586 - +0.6
303,586 - -0.5
303,586 - -1.0
303,586 - -0.1
303,704 + -1.6
303,704 + -0.8
303,704 + -1.0
303,704 + +0.1
303,704 + -0.1
303,704 + -0.0
303,704 + +2.1
303,705 - -1.9
303,705 - -2.0
303,705 - -0.2
303,705 - +0.4
303,705 - -1.0
303,705 - +0.8
303,705 - -1.2
303,705 - +1.5
303,935 + ABZR87_RS17570 0.65 +0.5
303,936 - ABZR87_RS17570 0.65 +0.4
304,081 + +0.7
304,082 - -0.8
304,082 - +0.3
304,082 - -1.0
304,082 - +0.2
304,082 - -0.5
304,082 - -1.4
304,082 - -0.1
304,226 + +0.2
304,226 + -0.1
304,304 + ABZR87_RS17575 0.14 -0.3
304,304 + ABZR87_RS17575 0.14 -1.6
304,304 + ABZR87_RS17575 0.14 +0.5
304,304 + ABZR87_RS17575 0.14 -0.3
304,305 - ABZR87_RS17575 0.14 +0.3
304,305 - ABZR87_RS17575 0.14 -1.5
304,305 - ABZR87_RS17575 0.14 -0.7
304,412 + ABZR87_RS17575 0.23 +0.7
304,412 + ABZR87_RS17575 0.23 -0.5
304,469 + ABZR87_RS17575 0.27 +0.9
304,481 + ABZR87_RS17575 0.28 -0.8
304,481 + ABZR87_RS17575 0.28 -0.2
304,481 + ABZR87_RS17575 0.28 -0.7
304,481 + ABZR87_RS17575 0.28 -0.3
304,481 + ABZR87_RS17575 0.28 -0.0
304,482 - ABZR87_RS17575 0.28 -0.3
304,706 + ABZR87_RS17575 0.45 +0.4
304,706 + ABZR87_RS17575 0.45 -0.2
304,707 - ABZR87_RS17575 0.45 +0.4
304,763 + ABZR87_RS17575 0.49 +0.6
304,763 + ABZR87_RS17575 0.49 -0.4
304,764 - ABZR87_RS17575 0.50 +0.7
304,764 - ABZR87_RS17575 0.50 -0.2
304,764 - ABZR87_RS17575 0.50 +1.2
304,874 + ABZR87_RS17575 0.58 +0.9
304,874 + ABZR87_RS17575 0.58 -0.0
304,875 - ABZR87_RS17575 0.58 +0.1
304,875 - ABZR87_RS17575 0.58 +1.6
304,875 - ABZR87_RS17575 0.58 +0.0
304,889 + ABZR87_RS17575 0.59 -0.3
304,890 - ABZR87_RS17575 0.59 +0.2
304,916 + ABZR87_RS17575 0.61 +0.3
304,916 + ABZR87_RS17575 0.61 -1.6
304,916 + ABZR87_RS17575 0.61 +0.6

Or see this region's nucleotide sequence