Experiment: KB with Piperic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2229 and Psyr_2230 are separated by 188 nucleotides Psyr_2230 and Psyr_2231 are separated by 160 nucleotides Psyr_2231 and Psyr_2232 are separated by 6 nucleotides
Psyr_2229: Psyr_2229 - TonB-dependent siderophore receptor, at 2,590,553 to 2,592,664
_2229
Psyr_2230: Psyr_2230 - Mn2+/Fe2+ transporter, NRAMP family, at 2,592,853 to 2,594,154
_2230
Psyr_2231: Psyr_2231 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase, at 2,594,315 to 2,595,064
_2231
Psyr_2232: Psyr_2232 - Major facilitator superfamily, at 2,595,071 to 2,596,369
_2232
Position (kb)
2592
2593
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2591.984 kb on + strand, within Psyr_2229 at 2591.984 kb on + strand, within Psyr_2229 at 2591.984 kb on + strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2591.985 kb on - strand, within Psyr_2229 at 2592.124 kb on + strand, within Psyr_2229 at 2592.223 kb on + strand, within Psyr_2229 at 2592.224 kb on - strand, within Psyr_2229 at 2592.233 kb on - strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.288 kb on + strand, within Psyr_2229 at 2592.289 kb on - strand, within Psyr_2229 at 2592.328 kb on - strand, within Psyr_2229 at 2592.349 kb on + strand, within Psyr_2229 at 2592.741 kb on + strand at 2592.741 kb on + strand at 2592.742 kb on - strand at 2592.754 kb on + strand at 2592.793 kb on + strand at 2592.794 kb on - strand at 2592.947 kb on + strand at 2592.948 kb on - strand at 2592.988 kb on + strand, within Psyr_2230 at 2593.021 kb on - strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.271 kb on + strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.272 kb on - strand, within Psyr_2230 at 2593.478 kb on + strand, within Psyr_2230 at 2593.479 kb on - strand, within Psyr_2230 at 2593.495 kb on - strand, within Psyr_2230 at 2593.510 kb on + strand, within Psyr_2230 at 2593.694 kb on + strand, within Psyr_2230 at 2593.694 kb on + strand, within Psyr_2230 at 2593.694 kb on + strand, within Psyr_2230 at 2593.695 kb on - strand, within Psyr_2230 at 2593.695 kb on - strand, within Psyr_2230 at 2593.695 kb on - strand, within Psyr_2230 at 2593.698 kb on + strand, within Psyr_2230 at 2593.729 kb on + strand, within Psyr_2230 at 2593.730 kb on - strand, within Psyr_2230 at 2593.730 kb on - strand, within Psyr_2230 at 2593.808 kb on + strand, within Psyr_2230 at 2593.808 kb on + strand, within Psyr_2230 at 2593.808 kb on + strand, within Psyr_2230 at 2593.809 kb on - strand, within Psyr_2230 at 2594.000 kb on + strand, within Psyr_2230 at 2594.001 kb on - strand, within Psyr_2230 at 2594.001 kb on - strand, within Psyr_2230 at 2594.179 kb on + strand at 2594.179 kb on + strand at 2594.180 kb on - strand at 2594.246 kb on - strand at 2594.246 kb on - strand at 2594.249 kb on + strand at 2594.249 kb on + strand at 2594.249 kb on + strand at 2594.250 kb on - strand at 2594.250 kb on - strand at 2594.276 kb on + strand at 2594.654 kb on + strand, within Psyr_2231 at 2594.655 kb on - strand, within Psyr_2231 at 2594.705 kb on + strand, within Psyr_2231 at 2594.706 kb on - strand, within Psyr_2231 at 2594.804 kb on + strand, within Psyr_2231 at 2594.843 kb on + strand, within Psyr_2231 at 2594.843 kb on + strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231 at 2594.844 kb on - strand, within Psyr_2231
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperic 10 mM remove 2,591,984 + Psyr_2229 0.68 -1.2 2,591,984 + Psyr_2229 0.68 +1.9 2,591,984 + Psyr_2229 0.68 +1.0 2,591,985 - Psyr_2229 0.68 -1.0 2,591,985 - Psyr_2229 0.68 +1.7 2,591,985 - Psyr_2229 0.68 -0.7 2,591,985 - Psyr_2229 0.68 +0.6 2,592,124 + Psyr_2229 0.74 +0.1 2,592,223 + Psyr_2229 0.79 +1.3 2,592,224 - Psyr_2229 0.79 -0.1 2,592,233 - Psyr_2229 0.80 +0.7 2,592,288 + Psyr_2229 0.82 -0.2 2,592,288 + Psyr_2229 0.82 +0.6 2,592,288 + Psyr_2229 0.82 -0.2 2,592,289 - Psyr_2229 0.82 -1.9 2,592,328 - Psyr_2229 0.84 -1.9 2,592,349 + Psyr_2229 0.85 +0.8 2,592,741 + -2.0 2,592,741 + +1.8 2,592,742 - +0.3 2,592,754 + +1.6 2,592,793 + +2.3 2,592,794 - +2.5 2,592,947 + +1.7 2,592,948 - +1.5 2,592,988 + Psyr_2230 0.10 +1.3 2,593,021 - Psyr_2230 0.13 -1.7 2,593,271 + Psyr_2230 0.32 -1.3 2,593,271 + Psyr_2230 0.32 +0.3 2,593,271 + Psyr_2230 0.32 -1.1 2,593,271 + Psyr_2230 0.32 -1.3 2,593,271 + Psyr_2230 0.32 +1.3 2,593,271 + Psyr_2230 0.32 +0.3 2,593,271 + Psyr_2230 0.32 +0.4 2,593,272 - Psyr_2230 0.32 -0.4 2,593,272 - Psyr_2230 0.32 +0.5 2,593,272 - Psyr_2230 0.32 +0.2 2,593,478 + Psyr_2230 0.48 +0.3 2,593,479 - Psyr_2230 0.48 +0.0 2,593,495 - Psyr_2230 0.49 -0.8 2,593,510 + Psyr_2230 0.50 +0.4 2,593,694 + Psyr_2230 0.65 +0.7 2,593,694 + Psyr_2230 0.65 -1.9 2,593,694 + Psyr_2230 0.65 -0.0 2,593,695 - Psyr_2230 0.65 +0.5 2,593,695 - Psyr_2230 0.65 -0.4 2,593,695 - Psyr_2230 0.65 -3.2 2,593,698 + Psyr_2230 0.65 -0.8 2,593,729 + Psyr_2230 0.67 +0.5 2,593,730 - Psyr_2230 0.67 -2.2 2,593,730 - Psyr_2230 0.67 -0.8 2,593,808 + Psyr_2230 0.73 -0.0 2,593,808 + Psyr_2230 0.73 -0.5 2,593,808 + Psyr_2230 0.73 +0.3 2,593,809 - Psyr_2230 0.73 -2.7 2,594,000 + Psyr_2230 0.88 -1.6 2,594,001 - Psyr_2230 0.88 -1.4 2,594,001 - Psyr_2230 0.88 -1.4 2,594,179 + +1.8 2,594,179 + +2.3 2,594,180 - +0.2 2,594,246 - +1.3 2,594,246 - -0.3 2,594,249 + +1.3 2,594,249 + -1.0 2,594,249 + -1.0 2,594,250 - +0.8 2,594,250 - -0.8 2,594,276 + +0.5 2,594,654 + Psyr_2231 0.45 -0.0 2,594,655 - Psyr_2231 0.45 +2.7 2,594,705 + Psyr_2231 0.52 -0.8 2,594,706 - Psyr_2231 0.52 -0.6 2,594,804 + Psyr_2231 0.65 -0.7 2,594,843 + Psyr_2231 0.70 +2.3 2,594,843 + Psyr_2231 0.70 -0.9 2,594,844 - Psyr_2231 0.71 -0.5 2,594,844 - Psyr_2231 0.71 -1.3 2,594,844 - Psyr_2231 0.71 -0.5
Or see this region's nucleotide sequence