Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS32190 and AZOBR_RS32195 are separated by 91 nucleotides AZOBR_RS32195 and AZOBR_RS32200 are separated by 96 nucleotides AZOBR_RS32200 and AZOBR_RS32205 are separated by 125 nucleotides AZOBR_RS32205 and AZOBR_RS32210 overlap by 4 nucleotides AZOBR_RS32210 and AZOBR_RS32215 are separated by 304 nucleotides AZOBR_RS32215 and AZOBR_RS32220 are separated by 22 nucleotides
AZOBR_RS32190: AZOBR_RS32190 - glycerol-3-phosphate dehydrogenase subunit C, at 589,497 to 589,766
_RS32190
AZOBR_RS32195: AZOBR_RS32195 - glycerol-3-phosphate dehydrogenase subunit C, at 589,858 to 590,124
_RS32195
AZOBR_RS32200: AZOBR_RS32200 - glycerol-3-phosphate dehydrogenase subunit C, at 590,221 to 590,493
_RS32200
AZOBR_RS32205: AZOBR_RS32205 - hypothetical protein, at 590,619 to 590,852
_RS32205
AZOBR_RS32210: AZOBR_RS32210 - hypothetical protein, at 590,849 to 591,100
_RS32210
AZOBR_RS32215: AZOBR_RS32215 - hypothetical protein, at 591,405 to 591,755
_RS32215
AZOBR_RS32220: AZOBR_RS32220 - hypothetical protein, at 591,778 to 592,857
_RS32220
Position (kb) 590
591 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 589.665 kb on + strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.666 kb on - strand, within AZOBR_RS32190 at 589.849 kb on + strand at 589.960 kb on + strand, within AZOBR_RS32195 at 589.961 kb on - strand, within AZOBR_RS32195 at 590.026 kb on + strand, within AZOBR_RS32195 at 590.026 kb on + strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.027 kb on - strand, within AZOBR_RS32195 at 590.072 kb on - strand, within AZOBR_RS32195 at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.206 kb on + strand at 590.207 kb on - strand at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.389 kb on + strand, within AZOBR_RS32200 at 590.390 kb on - strand, within AZOBR_RS32200 at 590.491 kb on + strand at 590.491 kb on + strand at 590.492 kb on - strand at 590.566 kb on - strand at 590.566 kb on - strand at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.715 kb on + strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 590.716 kb on - strand, within AZOBR_RS32205 at 591.449 kb on - strand, within AZOBR_RS32215 at 591.449 kb on - strand, within AZOBR_RS32215 at 591.489 kb on - strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.513 kb on + strand, within AZOBR_RS32215 at 591.514 kb on - strand, within AZOBR_RS32215 at 591.514 kb on - strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.645 kb on + strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.646 kb on - strand, within AZOBR_RS32215 at 591.737 kb on + strand at 591.737 kb on + strand at 591.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 589,665 + AZOBR_RS32190 0.62 -0.5 589,666 - AZOBR_RS32190 0.63 +1.7 589,666 - AZOBR_RS32190 0.63 -2.2 589,666 - AZOBR_RS32190 0.63 +0.1 589,849 + -1.7 589,960 + AZOBR_RS32195 0.38 +1.2 589,961 - AZOBR_RS32195 0.39 -1.5 590,026 + AZOBR_RS32195 0.63 +0.6 590,026 + AZOBR_RS32195 0.63 -0.9 590,027 - AZOBR_RS32195 0.63 -0.3 590,027 - AZOBR_RS32195 0.63 +0.5 590,027 - AZOBR_RS32195 0.63 -3.0 590,072 - AZOBR_RS32195 0.80 -1.2 590,206 + +1.1 590,206 + -0.8 590,206 + -0.5 590,206 + -0.2 590,206 + +0.2 590,206 + +0.4 590,207 - +1.6 590,389 + AZOBR_RS32200 0.62 -0.8 590,389 + AZOBR_RS32200 0.62 -0.2 590,389 + AZOBR_RS32200 0.62 -0.7 590,389 + AZOBR_RS32200 0.62 +2.0 590,390 - AZOBR_RS32200 0.62 -1.8 590,491 + +1.3 590,491 + +0.5 590,492 - -1.1 590,566 - -1.3 590,566 - -0.7 590,715 + AZOBR_RS32205 0.41 +1.2 590,715 + AZOBR_RS32205 0.41 -0.2 590,715 + AZOBR_RS32205 0.41 +0.4 590,715 + AZOBR_RS32205 0.41 +1.3 590,715 + AZOBR_RS32205 0.41 +0.4 590,715 + AZOBR_RS32205 0.41 -1.6 590,716 - AZOBR_RS32205 0.41 -1.8 590,716 - AZOBR_RS32205 0.41 +0.4 590,716 - AZOBR_RS32205 0.41 +1.5 591,449 - AZOBR_RS32215 0.13 -0.3 591,449 - AZOBR_RS32215 0.13 +0.6 591,489 - AZOBR_RS32215 0.24 +0.6 591,513 + AZOBR_RS32215 0.31 -1.2 591,513 + AZOBR_RS32215 0.31 -0.6 591,513 + AZOBR_RS32215 0.31 -0.0 591,514 - AZOBR_RS32215 0.31 -1.0 591,514 - AZOBR_RS32215 0.31 -0.3 591,645 + AZOBR_RS32215 0.68 -0.6 591,645 + AZOBR_RS32215 0.68 -0.6 591,645 + AZOBR_RS32215 0.68 +1.0 591,646 - AZOBR_RS32215 0.69 -0.1 591,646 - AZOBR_RS32215 0.69 +2.1 591,646 - AZOBR_RS32215 0.69 +1.3 591,646 - AZOBR_RS32215 0.69 +0.5 591,646 - AZOBR_RS32215 0.69 +0.4 591,737 + -0.9 591,737 + -0.4 591,737 + +0.7
Or see this region's nucleotide sequence