Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS18840 and AZOBR_RS18845 are separated by 123 nucleotides AZOBR_RS18845 and AZOBR_RS18850 are separated by 184 nucleotides AZOBR_RS18850 and AZOBR_RS18855 are separated by 209 nucleotides AZOBR_RS18855 and AZOBR_RS18860 overlap by 4 nucleotides
AZOBR_RS18840: AZOBR_RS18840 - tRNA-Leu, at 999,379 to 999,465
_RS18840
AZOBR_RS18845: AZOBR_RS18845 - 3'-5' exonuclease, at 999,589 to 1,000,215
_RS18845
AZOBR_RS18850: AZOBR_RS18850 - arabinose 5-phosphate isomerase, at 1,000,400 to 1,001,428
_RS18850
AZOBR_RS18855: AZOBR_RS18855 - hypothetical protein, at 1,001,638 to 1,002,192
_RS18855
AZOBR_RS18860: AZOBR_RS18860 - hypothetical protein, at 1,002,189 to 1,003,088
_RS18860
Position (kb) 1000
1001
1002 Strain fitness (log2 ratio) -2
-1
0
1 at 999.466 kb on + strand at 999.466 kb on + strand at 999.470 kb on + strand at 999.519 kb on + strand at 999.568 kb on + strand at 999.568 kb on + strand at 1000.248 kb on + strand at 1000.248 kb on + strand at 1000.298 kb on + strand at 1001.092 kb on + strand, within AZOBR_RS18850 at 1001.092 kb on + strand, within AZOBR_RS18850 at 1001.092 kb on + strand, within AZOBR_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 999,466 + +0.1 999,466 + -0.7 999,470 + -0.8 999,519 + -0.9 999,568 + -0.2 999,568 + +0.1 1,000,248 + +0.4 1,000,248 + -0.0 1,000,298 + -0.6 1,001,092 + AZOBR_RS18850 0.67 -0.5 1,001,092 + AZOBR_RS18850 0.67 -2.1 1,001,092 + AZOBR_RS18850 0.67 -1.3
Or see this region's nucleotide sequence