Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03865 and AZOBR_RS00005 are separated by 133 nucleotides AZOBR_RS00005 and AZOBR_RS03870 are separated by 96 nucleotides AZOBR_RS03870 and AZOBR_RS03875 are separated by 3 nucleotides AZOBR_RS03875 and AZOBR_RS03880 are separated by 59 nucleotides
AZOBR_RS03865: AZOBR_RS03865 - cytochrome C, at 815,905 to 816,444
_RS03865
AZOBR_RS00005: AZOBR_RS00005 - SRP_bact, at 816,578 to 816,680
_RS00005
AZOBR_RS03870: AZOBR_RS03870 - DNA polymerase III subunit gamma/tau, at 816,777 to 818,633
_RS03870
AZOBR_RS03875: AZOBR_RS03875 - hypothetical protein, at 818,637 to 818,960
_RS03875
AZOBR_RS03880: AZOBR_RS03880 - recombinase RecR, at 819,020 to 819,613
_RS03880
Position (kb) 816
817
818
819 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 816.316 kb on + strand, within AZOBR_RS03865 at 816.687 kb on + strand at 818.573 kb on + strand at 818.573 kb on + strand at 818.601 kb on + strand at 818.601 kb on + strand at 818.602 kb on - strand at 818.959 kb on - strand at 818.959 kb on - strand at 818.980 kb on + strand at 819.326 kb on + strand, within AZOBR_RS03880 at 819.326 kb on + strand, within AZOBR_RS03880 at 819.326 kb on + strand, within AZOBR_RS03880 at 819.326 kb on + strand, within AZOBR_RS03880 at 819.327 kb on - strand, within AZOBR_RS03880 at 819.327 kb on - strand, within AZOBR_RS03880 at 819.327 kb on - strand, within AZOBR_RS03880 at 819.327 kb on - strand, within AZOBR_RS03880 at 819.560 kb on + strand at 819.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 816,316 + AZOBR_RS03865 0.76 -0.9 816,687 + -1.3 818,573 + +0.2 818,573 + +1.5 818,601 + -0.1 818,601 + +1.7 818,602 - -1.6 818,959 - -3.5 818,959 - -2.6 818,980 + -0.4 819,326 + AZOBR_RS03880 0.52 -1.2 819,326 + AZOBR_RS03880 0.52 -2.1 819,326 + AZOBR_RS03880 0.52 -3.9 819,326 + AZOBR_RS03880 0.52 -0.9 819,327 - AZOBR_RS03880 0.52 -3.4 819,327 - AZOBR_RS03880 0.52 +2.5 819,327 - AZOBR_RS03880 0.52 -4.2 819,327 - AZOBR_RS03880 0.52 -0.3 819,560 + -1.4 819,611 + +1.4
Or see this region's nucleotide sequence