Experiment: BHIS with Dimetridazole 0.0015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1919 and BT1920 are separated by 366 nucleotides BT1920 and BT1921 are separated by 3 nucleotides BT1921 and BT1922 are separated by 31 nucleotides BT1922 and BT1923 are separated by 121 nucleotides
BT1919: BT1919 - conserved hypothetical protein with a conserved domain (NCBI ptt file), at 2,409,425 to 2,410,435
BT1919
BT1920: BT1920 - hypothetical protein (NCBI ptt file), at 2,410,802 to 2,411,035
BT1920
BT1921: BT1921 - hypothetical protein (NCBI ptt file), at 2,411,039 to 2,411,278
BT1921
BT1922: BT1922 - putative N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 2,411,310 to 2,412,080
BT1922
BT1923: BT1923 - O-acetylhomoserine (thiol)-lyase (NCBI ptt file), at 2,412,202 to 2,413,482
BT1923
Position (kb)
2411
2412
2413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2410.386 kb on + strand at 2410.406 kb on + strand at 2410.413 kb on - strand at 2410.565 kb on - strand at 2410.584 kb on - strand at 2410.657 kb on + strand at 2410.692 kb on + strand at 2410.706 kb on + strand at 2410.707 kb on - strand at 2410.711 kb on - strand at 2410.721 kb on - strand at 2410.727 kb on - strand at 2410.737 kb on - strand at 2410.945 kb on + strand, within BT1920 at 2410.945 kb on + strand, within BT1920 at 2410.949 kb on + strand, within BT1920 at 2410.950 kb on - strand, within BT1920 at 2410.989 kb on + strand, within BT1920 at 2410.989 kb on + strand, within BT1920 at 2410.990 kb on - strand, within BT1920 at 2410.991 kb on + strand, within BT1920 at 2410.991 kb on + strand, within BT1920 at 2410.992 kb on - strand, within BT1920 at 2410.992 kb on - strand, within BT1920 at 2410.993 kb on + strand, within BT1920 at 2410.993 kb on + strand, within BT1920 at 2410.994 kb on - strand, within BT1920 at 2411.144 kb on + strand, within BT1921 at 2411.144 kb on + strand, within BT1921 at 2411.145 kb on - strand, within BT1921 at 2411.145 kb on - strand, within BT1921 at 2411.146 kb on + strand, within BT1921 at 2411.196 kb on + strand, within BT1921 at 2411.199 kb on - strand, within BT1921 at 2411.278 kb on + strand at 2411.312 kb on - strand at 2411.323 kb on - strand at 2411.364 kb on + strand at 2411.365 kb on - strand at 2411.390 kb on - strand, within BT1922 at 2411.393 kb on + strand, within BT1922 at 2411.487 kb on - strand, within BT1922 at 2411.553 kb on - strand, within BT1922 at 2411.558 kb on + strand, within BT1922 at 2411.559 kb on - strand, within BT1922 at 2411.615 kb on - strand, within BT1922 at 2411.620 kb on + strand, within BT1922 at 2411.620 kb on + strand, within BT1922 at 2411.621 kb on - strand, within BT1922 at 2411.621 kb on - strand, within BT1922 at 2411.621 kb on - strand, within BT1922 at 2411.694 kb on + strand, within BT1922 at 2411.699 kb on - strand, within BT1922 at 2411.699 kb on - strand, within BT1922 at 2411.737 kb on - strand, within BT1922 at 2411.754 kb on - strand, within BT1922 at 2411.861 kb on + strand, within BT1922 at 2411.862 kb on - strand, within BT1922 at 2411.866 kb on + strand, within BT1922 at 2411.867 kb on - strand at 2411.935 kb on + strand, within BT1922 at 2411.938 kb on + strand, within BT1922 at 2412.048 kb on - strand at 2412.064 kb on + strand at 2412.091 kb on + strand at 2412.097 kb on - strand at 2412.102 kb on + strand at 2412.201 kb on + strand at 2412.287 kb on - strand at 2412.295 kb on - strand at 2412.388 kb on - strand, within BT1923 at 2412.438 kb on + strand, within BT1923 at 2412.444 kb on + strand, within BT1923 at 2412.445 kb on - strand, within BT1923 at 2412.586 kb on - strand, within BT1923 at 2412.586 kb on - strand, within BT1923 at 2412.639 kb on + strand, within BT1923 at 2412.753 kb on + strand, within BT1923 at 2412.768 kb on + strand, within BT1923 at 2412.768 kb on + strand, within BT1923 at 2412.769 kb on - strand, within BT1923 at 2412.769 kb on - strand, within BT1923 at 2412.827 kb on - strand, within BT1923 at 2412.852 kb on - strand, within BT1923 at 2412.937 kb on + strand, within BT1923 at 2412.937 kb on + strand, within BT1923 at 2412.952 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.980 kb on - strand, within BT1923 at 2412.980 kb on - strand, within BT1923
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0015625 mM remove 2,410,386 + -2.0 2,410,406 + +0.3 2,410,413 - -1.3 2,410,565 - -0.0 2,410,584 - -2.6 2,410,657 + -0.0 2,410,692 + -1.2 2,410,706 + -1.3 2,410,707 - -1.6 2,410,711 - -1.4 2,410,721 - -0.9 2,410,727 - -0.9 2,410,737 - +1.6 2,410,945 + BT1920 0.61 -2.8 2,410,945 + BT1920 0.61 -1.6 2,410,949 + BT1920 0.63 -2.0 2,410,950 - BT1920 0.63 -0.0 2,410,989 + BT1920 0.80 -1.2 2,410,989 + BT1920 0.80 -0.9 2,410,990 - BT1920 0.80 +0.4 2,410,991 + BT1920 0.81 -0.6 2,410,991 + BT1920 0.81 -1.7 2,410,992 - BT1920 0.81 -1.9 2,410,992 - BT1920 0.81 -0.0 2,410,993 + BT1920 0.82 -2.2 2,410,993 + BT1920 0.82 -0.7 2,410,994 - BT1920 0.82 -1.9 2,411,144 + BT1921 0.44 -1.5 2,411,144 + BT1921 0.44 -4.0 2,411,145 - BT1921 0.44 -1.6 2,411,145 - BT1921 0.44 -1.0 2,411,146 + BT1921 0.45 -0.0 2,411,196 + BT1921 0.65 -1.0 2,411,199 - BT1921 0.67 -2.9 2,411,278 + -2.8 2,411,312 - -3.3 2,411,323 - -0.4 2,411,364 + -1.0 2,411,365 - -1.3 2,411,390 - BT1922 0.10 -1.0 2,411,393 + BT1922 0.11 -1.0 2,411,487 - BT1922 0.23 -1.0 2,411,553 - BT1922 0.32 -1.6 2,411,558 + BT1922 0.32 -1.8 2,411,559 - BT1922 0.32 -1.8 2,411,615 - BT1922 0.40 -0.0 2,411,620 + BT1922 0.40 +1.0 2,411,620 + BT1922 0.40 -1.0 2,411,621 - BT1922 0.40 -1.6 2,411,621 - BT1922 0.40 -1.3 2,411,621 - BT1922 0.40 -0.6 2,411,694 + BT1922 0.50 -2.4 2,411,699 - BT1922 0.50 -2.6 2,411,699 - BT1922 0.50 -2.8 2,411,737 - BT1922 0.55 -0.8 2,411,754 - BT1922 0.58 -2.3 2,411,861 + BT1922 0.71 -4.0 2,411,862 - BT1922 0.72 -1.0 2,411,866 + BT1922 0.72 -2.0 2,411,867 - -1.0 2,411,935 + BT1922 0.81 -2.9 2,411,938 + BT1922 0.81 -0.9 2,412,048 - -0.4 2,412,064 + -1.6 2,412,091 + -1.0 2,412,097 - -2.0 2,412,102 + -2.0 2,412,201 + -2.3 2,412,287 - -2.0 2,412,295 - -2.0 2,412,388 - BT1923 0.15 -2.0 2,412,438 + BT1923 0.18 -3.2 2,412,444 + BT1923 0.19 -1.2 2,412,445 - BT1923 0.19 -3.2 2,412,586 - BT1923 0.30 -2.4 2,412,586 - BT1923 0.30 -1.0 2,412,639 + BT1923 0.34 -1.0 2,412,753 + BT1923 0.43 -1.6 2,412,768 + BT1923 0.44 -1.2 2,412,768 + BT1923 0.44 -1.6 2,412,769 - BT1923 0.44 -1.0 2,412,769 - BT1923 0.44 -3.0 2,412,827 - BT1923 0.49 -2.3 2,412,852 - BT1923 0.51 -1.4 2,412,937 + BT1923 0.57 -0.7 2,412,937 + BT1923 0.57 -1.0 2,412,952 + BT1923 0.59 -0.0 2,412,979 + BT1923 0.61 +0.3 2,412,979 + BT1923 0.61 +1.3 2,412,979 + BT1923 0.61 -1.6 2,412,979 + BT1923 0.61 -1.7 2,412,980 - BT1923 0.61 -1.8 2,412,980 - BT1923 0.61 -1.6
Or see this region's nucleotide sequence