Experiment: BHIS with Dimetridazole 0.0015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1059 and BT1060 are separated by 210 nucleotides BT1060 and BT1061 are separated by 241 nucleotides
BT1059: BT1059 - putative UDP-glucose 4-epimerase (NCBI ptt file), at 1,345,817 to 1,346,746
BT1059
BT1060: BT1060 - hypothetical protein (NCBI ptt file), at 1,346,957 to 1,347,910
BT1060
BT1061: BT1061 - hypothetical protein (NCBI ptt file), at 1,348,152 to 1,349,111
BT1061
Position (kb)
1346
1347
1348 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1346.104 kb on + strand, within BT1059 at 1346.105 kb on - strand, within BT1059 at 1346.140 kb on - strand, within BT1059 at 1346.142 kb on - strand, within BT1059 at 1346.172 kb on - strand, within BT1059 at 1346.203 kb on + strand, within BT1059 at 1346.227 kb on + strand, within BT1059 at 1346.275 kb on - strand, within BT1059 at 1346.317 kb on - strand, within BT1059 at 1346.622 kb on - strand, within BT1059 at 1346.623 kb on + strand, within BT1059 at 1346.623 kb on + strand, within BT1059 at 1346.624 kb on - strand, within BT1059 at 1346.624 kb on - strand, within BT1059 at 1346.662 kb on + strand at 1346.662 kb on + strand at 1346.682 kb on - strand at 1346.736 kb on - strand at 1346.798 kb on + strand at 1346.830 kb on + strand at 1346.919 kb on + strand at 1346.971 kb on + strand at 1347.030 kb on + strand at 1347.047 kb on + strand at 1347.047 kb on + strand at 1347.047 kb on + strand at 1347.050 kb on - strand at 1347.064 kb on + strand, within BT1060 at 1347.163 kb on + strand, within BT1060 at 1347.164 kb on - strand, within BT1060 at 1347.199 kb on - strand, within BT1060 at 1347.202 kb on - strand, within BT1060 at 1347.202 kb on - strand, within BT1060 at 1347.230 kb on + strand, within BT1060 at 1347.295 kb on + strand, within BT1060 at 1347.327 kb on + strand, within BT1060 at 1347.394 kb on + strand, within BT1060 at 1347.533 kb on - strand, within BT1060 at 1347.535 kb on - strand, within BT1060 at 1347.552 kb on + strand, within BT1060 at 1347.552 kb on + strand, within BT1060 at 1347.667 kb on + strand, within BT1060 at 1347.671 kb on + strand, within BT1060 at 1347.671 kb on + strand, within BT1060 at 1347.701 kb on + strand, within BT1060 at 1347.707 kb on + strand, within BT1060 at 1347.722 kb on - strand, within BT1060 at 1347.726 kb on + strand, within BT1060 at 1347.733 kb on + strand, within BT1060 at 1347.757 kb on + strand, within BT1060 at 1347.757 kb on + strand, within BT1060 at 1347.758 kb on - strand, within BT1060 at 1347.769 kb on + strand, within BT1060 at 1347.907 kb on - strand at 1347.981 kb on + strand at 1347.981 kb on + strand at 1348.004 kb on - strand at 1348.027 kb on + strand at 1348.106 kb on - strand at 1348.120 kb on - strand at 1348.125 kb on - strand at 1348.137 kb on - strand at 1348.358 kb on + strand, within BT1061 at 1348.359 kb on - strand, within BT1061 at 1348.426 kb on - strand, within BT1061 at 1348.428 kb on + strand, within BT1061 at 1348.456 kb on + strand, within BT1061 at 1348.493 kb on - strand, within BT1061 at 1348.496 kb on + strand, within BT1061 at 1348.537 kb on - strand, within BT1061 at 1348.543 kb on - strand, within BT1061 at 1348.670 kb on - strand, within BT1061 at 1348.690 kb on - strand, within BT1061 at 1348.801 kb on - strand, within BT1061 at 1348.835 kb on + strand, within BT1061 at 1348.909 kb on - strand, within BT1061 at 1348.909 kb on - strand, within BT1061
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0015625 mM remove 1,346,104 + BT1059 0.31 -1.6 1,346,105 - BT1059 0.31 -1.5 1,346,140 - BT1059 0.35 -3.0 1,346,142 - BT1059 0.35 -2.0 1,346,172 - BT1059 0.38 -0.2 1,346,203 + BT1059 0.42 -1.6 1,346,227 + BT1059 0.44 -0.2 1,346,275 - BT1059 0.49 -0.2 1,346,317 - BT1059 0.54 -0.2 1,346,622 - BT1059 0.87 -1.1 1,346,623 + BT1059 0.87 -0.6 1,346,623 + BT1059 0.87 -2.8 1,346,624 - BT1059 0.87 -3.0 1,346,624 - BT1059 0.87 -1.2 1,346,662 + -0.2 1,346,662 + -0.2 1,346,682 - -1.8 1,346,736 - -0.2 1,346,798 + -1.7 1,346,830 + -1.8 1,346,919 + -0.2 1,346,971 + -1.4 1,347,030 + -1.2 1,347,047 + -1.5 1,347,047 + +0.1 1,347,047 + -1.0 1,347,050 - -1.2 1,347,064 + BT1060 0.11 -0.7 1,347,163 + BT1060 0.22 -0.2 1,347,164 - BT1060 0.22 +0.8 1,347,199 - BT1060 0.25 -2.3 1,347,202 - BT1060 0.26 -1.2 1,347,202 - BT1060 0.26 -2.6 1,347,230 + BT1060 0.29 -1.9 1,347,295 + BT1060 0.35 -0.6 1,347,327 + BT1060 0.39 -0.8 1,347,394 + BT1060 0.46 -0.8 1,347,533 - BT1060 0.60 -2.6 1,347,535 - BT1060 0.61 -2.4 1,347,552 + BT1060 0.62 -0.2 1,347,552 + BT1060 0.62 -1.2 1,347,667 + BT1060 0.74 -1.4 1,347,671 + BT1060 0.75 +0.1 1,347,671 + BT1060 0.75 -2.2 1,347,701 + BT1060 0.78 -2.3 1,347,707 + BT1060 0.79 -1.0 1,347,722 - BT1060 0.80 -3.8 1,347,726 + BT1060 0.81 -2.4 1,347,733 + BT1060 0.81 -0.2 1,347,757 + BT1060 0.84 -1.8 1,347,757 + BT1060 0.84 -0.2 1,347,758 - BT1060 0.84 -2.6 1,347,769 + BT1060 0.85 -1.8 1,347,907 - -2.2 1,347,981 + -2.6 1,347,981 + -2.2 1,348,004 - -0.2 1,348,027 + -2.6 1,348,106 - -1.2 1,348,120 - -1.2 1,348,125 - -2.2 1,348,137 - +0.4 1,348,358 + BT1061 0.21 -0.4 1,348,359 - BT1061 0.22 +0.8 1,348,426 - BT1061 0.29 -1.2 1,348,428 + BT1061 0.29 -0.2 1,348,456 + BT1061 0.32 -0.2 1,348,493 - BT1061 0.36 -1.5 1,348,496 + BT1061 0.36 -2.2 1,348,537 - BT1061 0.40 -0.8 1,348,543 - BT1061 0.41 -1.6 1,348,670 - BT1061 0.54 -0.2 1,348,690 - BT1061 0.56 -1.2 1,348,801 - BT1061 0.68 -2.2 1,348,835 + BT1061 0.71 -1.0 1,348,909 - BT1061 0.79 -1.8 1,348,909 - BT1061 0.79 -1.2
Or see this region's nucleotide sequence