Experiment: KB with Palmatine chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1921 and Psyr_1922 are separated by 15 nucleotides Psyr_1922 and Psyr_1923 are separated by 43 nucleotides Psyr_1923 and Psyr_1924 overlap by 26 nucleotides Psyr_1924 and Psyr_1925 are separated by 29 nucleotides
Psyr_1921: Psyr_1921 - Alpha/beta hydrolase fold protein, at 2,194,112 to 2,195,221
_1921
Psyr_1922: Psyr_1922 - UBA/THIF-type NAD/FAD binding fold protein, at 2,195,237 to 2,196,166
_1922
Psyr_1923: Psyr_1923 - hypothetical protein, at 2,196,210 to 2,197,433
_1923
Psyr_1924: Psyr_1924 - hypothetical protein, at 2,197,408 to 2,198,067
_1924
Psyr_1925: Psyr_1925 - diaminobutyrate aminotransferase apoenzyme, at 2,198,097 to 2,199,377
_1925
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2195.219 kb on + strand at 2195.219 kb on + strand at 2195.220 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.445 kb on + strand, within Psyr_1922 at 2195.445 kb on + strand, within Psyr_1922 at 2195.446 kb on - strand, within Psyr_1922 at 2195.480 kb on + strand, within Psyr_1922 at 2195.533 kb on + strand, within Psyr_1922 at 2195.609 kb on - strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.693 kb on + strand, within Psyr_1922 at 2195.694 kb on - strand, within Psyr_1922 at 2195.717 kb on - strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.886 kb on - strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.951 kb on - strand, within Psyr_1922 at 2196.036 kb on - strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand at 2196.066 kb on - strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.069 kb on - strand, within Psyr_1922 at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.086 kb on - strand at 2196.086 kb on - strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.295 kb on + strand at 2196.296 kb on - strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.459 kb on + strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.523 kb on - strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand at 2196.641 kb on - strand, within Psyr_1923 at 2196.641 kb on - strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.773 kb on + strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.871 kb on + strand, within Psyr_1923 at 2196.934 kb on + strand, within Psyr_1923 at 2196.935 kb on - strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2197.052 kb on + strand, within Psyr_1923 at 2197.053 kb on - strand, within Psyr_1923 at 2197.057 kb on + strand, within Psyr_1923 at 2197.094 kb on - strand, within Psyr_1923 at 2197.185 kb on - strand, within Psyr_1923 at 2197.226 kb on + strand, within Psyr_1923 at 2197.279 kb on + strand, within Psyr_1923 at 2197.331 kb on - strand at 2197.381 kb on - strand at 2197.383 kb on - strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.387 kb on - strand at 2197.387 kb on - strand at 2197.461 kb on + strand at 2197.461 kb on + strand at 2197.469 kb on + strand at 2197.469 kb on + strand at 2197.470 kb on - strand at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.539 kb on - strand, within Psyr_1924 at 2197.600 kb on + strand, within Psyr_1924 at 2197.800 kb on + strand, within Psyr_1924 at 2197.855 kb on + strand, within Psyr_1924 at 2197.884 kb on + strand, within Psyr_1924 at 2197.885 kb on - strand, within Psyr_1924 at 2197.902 kb on - strand, within Psyr_1924 at 2197.977 kb on + strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.066 kb on - strand at 2198.070 kb on + strand at 2198.118 kb on + strand at 2198.118 kb on + strand at 2198.119 kb on - strand at 2198.119 kb on - strand at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.295 kb on + strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.298 kb on - strand, within Psyr_1925 at 2198.378 kb on + strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine chloride 10 mM remove 2,195,219 + +2.2 2,195,219 + -1.0 2,195,220 - -0.8 2,195,292 - +1.4 2,195,292 - -0.2 2,195,292 - -0.6 2,195,445 + Psyr_1922 0.22 -0.9 2,195,445 + Psyr_1922 0.22 +0.7 2,195,446 - Psyr_1922 0.22 +1.7 2,195,480 + Psyr_1922 0.26 +2.1 2,195,533 + Psyr_1922 0.32 +0.5 2,195,609 - Psyr_1922 0.40 -0.3 2,195,610 + Psyr_1922 0.40 +2.0 2,195,610 + Psyr_1922 0.40 +1.6 2,195,610 + Psyr_1922 0.40 +2.7 2,195,610 + Psyr_1922 0.40 -2.6 2,195,611 - Psyr_1922 0.40 +0.7 2,195,611 - Psyr_1922 0.40 -0.6 2,195,611 - Psyr_1922 0.40 -0.2 2,195,611 - Psyr_1922 0.40 -1.0 2,195,675 + Psyr_1922 0.47 +0.3 2,195,675 + Psyr_1922 0.47 -2.5 2,195,676 - Psyr_1922 0.47 -1.0 2,195,676 - Psyr_1922 0.47 -2.6 2,195,676 - Psyr_1922 0.47 +1.0 2,195,693 + Psyr_1922 0.49 +1.1 2,195,694 - Psyr_1922 0.49 -1.1 2,195,717 - Psyr_1922 0.52 -1.1 2,195,777 + Psyr_1922 0.58 -0.2 2,195,777 + Psyr_1922 0.58 -1.0 2,195,777 + Psyr_1922 0.58 -1.3 2,195,777 + Psyr_1922 0.58 +0.4 2,195,777 + Psyr_1922 0.58 +0.4 2,195,777 + Psyr_1922 0.58 +0.0 2,195,777 + Psyr_1922 0.58 +0.0 2,195,777 + Psyr_1922 0.58 -1.6 2,195,778 - Psyr_1922 0.58 -1.3 2,195,778 - Psyr_1922 0.58 +0.2 2,195,778 - Psyr_1922 0.58 +0.7 2,195,885 + Psyr_1922 0.70 -1.7 2,195,885 + Psyr_1922 0.70 +0.5 2,195,885 + Psyr_1922 0.70 -0.7 2,195,886 - Psyr_1922 0.70 -1.7 2,195,950 + Psyr_1922 0.77 +0.0 2,195,950 + Psyr_1922 0.77 -1.0 2,195,951 - Psyr_1922 0.77 -3.3 2,196,036 - Psyr_1922 0.86 -0.6 2,196,065 + Psyr_1922 0.89 +1.4 2,196,065 + Psyr_1922 0.89 -0.6 2,196,065 + Psyr_1922 0.89 +1.2 2,196,065 + Psyr_1922 0.89 -1.2 2,196,065 + Psyr_1922 0.89 -0.9 2,196,066 - Psyr_1922 0.89 -0.7 2,196,066 - Psyr_1922 0.89 -0.6 2,196,066 - Psyr_1922 0.89 -0.6 2,196,066 - Psyr_1922 0.89 +0.9 2,196,066 - Psyr_1922 0.89 +0.5 2,196,066 - Psyr_1922 0.89 +1.2 2,196,066 - Psyr_1922 0.89 +1.2 2,196,066 - Psyr_1922 0.89 +1.0 2,196,066 - Psyr_1922 0.89 -1.9 2,196,066 - Psyr_1922 0.89 +1.0 2,196,066 - +2.0 2,196,066 - Psyr_1922 0.89 -0.6 2,196,068 + Psyr_1922 0.89 +0.8 2,196,068 + Psyr_1922 0.89 +2.0 2,196,068 + Psyr_1922 0.89 +0.2 2,196,069 - Psyr_1922 0.89 -2.0 2,196,085 + +0.1 2,196,085 + -0.6 2,196,085 + -1.0 2,196,086 - -1.3 2,196,086 - +0.0 2,196,100 + +0.4 2,196,100 + +0.7 2,196,100 + +0.3 2,196,101 - -0.3 2,196,101 - +2.9 2,196,101 - +2.2 2,196,104 + -0.4 2,196,104 + -1.2 2,196,104 + +1.7 2,196,245 + -0.2 2,196,245 + -1.2 2,196,245 + +0.7 2,196,245 + +1.0 2,196,246 - +0.0 2,196,246 - +0.3 2,196,246 - +1.8 2,196,246 - -1.0 2,196,246 - +0.0 2,196,295 + -1.6 2,196,296 - -1.8 2,196,304 + +0.4 2,196,304 + -2.1 2,196,304 + +0.5 2,196,405 + Psyr_1923 0.16 -0.1 2,196,405 + Psyr_1923 0.16 -0.7 2,196,405 + Psyr_1923 0.16 -0.2 2,196,405 + Psyr_1923 0.16 -1.6 2,196,406 - Psyr_1923 0.16 -0.8 2,196,406 - Psyr_1923 0.16 +1.1 2,196,459 + Psyr_1923 0.20 -0.1 2,196,505 - Psyr_1923 0.24 -2.4 2,196,505 - Psyr_1923 0.24 +0.4 2,196,522 + Psyr_1923 0.25 +1.0 2,196,522 + Psyr_1923 0.25 -2.1 2,196,523 - Psyr_1923 0.26 -0.6 2,196,540 + Psyr_1923 0.27 +2.0 2,196,540 + Psyr_1923 0.27 -1.3 2,196,540 + Psyr_1923 0.27 -3.3 2,196,540 + Psyr_1923 0.27 -2.2 2,196,595 - Psyr_1923 0.31 -0.1 2,196,595 - Psyr_1923 0.31 -0.4 2,196,604 + Psyr_1923 0.32 -1.9 2,196,604 + Psyr_1923 0.32 +0.4 2,196,605 - Psyr_1923 0.32 -0.3 2,196,605 - Psyr_1923 0.32 +1.0 2,196,605 - Psyr_1923 0.32 +0.0 2,196,605 - Psyr_1923 0.32 +1.0 2,196,605 - Psyr_1923 0.32 +0.7 2,196,638 + Psyr_1923 0.35 +1.8 2,196,638 + Psyr_1923 0.35 +1.2 2,196,640 + Psyr_1923 0.35 +0.7 2,196,640 + -1.2 2,196,641 - Psyr_1923 0.35 +0.2 2,196,641 - Psyr_1923 0.35 +0.4 2,196,771 + Psyr_1923 0.46 -1.0 2,196,771 + Psyr_1923 0.46 -0.2 2,196,771 + Psyr_1923 0.46 +0.3 2,196,771 + Psyr_1923 0.46 +0.5 2,196,771 + Psyr_1923 0.46 -0.8 2,196,771 + Psyr_1923 0.46 +0.3 2,196,772 - Psyr_1923 0.46 -1.4 2,196,772 - Psyr_1923 0.46 -0.2 2,196,772 - Psyr_1923 0.46 -0.9 2,196,773 + Psyr_1923 0.46 +2.8 2,196,774 - Psyr_1923 0.46 +0.0 2,196,774 - Psyr_1923 0.46 +0.7 2,196,774 - Psyr_1923 0.46 -1.0 2,196,774 - Psyr_1923 0.46 -1.2 2,196,774 - Psyr_1923 0.46 -0.7 2,196,774 - Psyr_1923 0.46 +0.0 2,196,871 + Psyr_1923 0.54 +1.0 2,196,934 + Psyr_1923 0.59 -0.2 2,196,935 - Psyr_1923 0.59 +1.2 2,196,938 + Psyr_1923 0.59 +3.7 2,196,938 + Psyr_1923 0.59 -1.1 2,196,938 + Psyr_1923 0.59 +3.0 2,196,938 + Psyr_1923 0.59 +1.2 2,196,938 + Psyr_1923 0.59 +3.0 2,196,938 + Psyr_1923 0.59 +0.4 2,196,939 - Psyr_1923 0.60 -0.5 2,196,939 - Psyr_1923 0.60 +0.1 2,196,939 - Psyr_1923 0.60 -0.2 2,196,939 - Psyr_1923 0.60 -1.0 2,196,939 - Psyr_1923 0.60 -0.9 2,196,939 - Psyr_1923 0.60 +1.4 2,197,052 + Psyr_1923 0.69 +0.2 2,197,053 - Psyr_1923 0.69 -0.7 2,197,057 + Psyr_1923 0.69 -1.2 2,197,094 - Psyr_1923 0.72 +0.3 2,197,185 - Psyr_1923 0.80 +0.3 2,197,226 + Psyr_1923 0.83 +0.1 2,197,279 + Psyr_1923 0.87 -0.8 2,197,331 - +0.1 2,197,381 - -0.5 2,197,383 - -1.9 2,197,386 + +0.4 2,197,386 + +1.0 2,197,386 + -1.1 2,197,386 + -1.4 2,197,386 + -0.7 2,197,386 + +0.0 2,197,387 - +0.2 2,197,387 - +0.5 2,197,461 + +1.0 2,197,461 + +0.2 2,197,469 + -0.1 2,197,469 + -0.2 2,197,470 - +0.4 2,197,526 - Psyr_1924 0.18 +0.0 2,197,526 - Psyr_1924 0.18 +0.7 2,197,526 - Psyr_1924 0.18 -1.2 2,197,526 - Psyr_1924 0.18 -1.4 2,197,526 - Psyr_1924 0.18 -2.7 2,197,539 - Psyr_1924 0.20 -3.2 2,197,600 + Psyr_1924 0.29 +0.3 2,197,800 + Psyr_1924 0.59 -1.0 2,197,855 + Psyr_1924 0.68 -0.3 2,197,884 + Psyr_1924 0.72 -0.8 2,197,885 - Psyr_1924 0.72 -1.2 2,197,902 - Psyr_1924 0.75 +0.0 2,197,977 + Psyr_1924 0.86 -0.6 2,197,978 - Psyr_1924 0.86 +0.6 2,197,978 - Psyr_1924 0.86 -0.1 2,198,047 + -1.0 2,198,047 + +2.6 2,198,047 + +0.4 2,198,047 + -3.2 2,198,048 - +3.6 2,198,048 - -1.0 2,198,048 - -0.6 2,198,048 - +1.0 2,198,066 - +3.3 2,198,070 + -1.2 2,198,118 + +1.0 2,198,118 + +0.2 2,198,119 - -0.5 2,198,119 - -2.4 2,198,253 + Psyr_1925 0.12 +0.7 2,198,253 + Psyr_1925 0.12 -1.2 2,198,253 + Psyr_1925 0.12 -0.9 2,198,295 + Psyr_1925 0.15 +0.4 2,198,296 - Psyr_1925 0.16 +0.3 2,198,296 - Psyr_1925 0.16 -0.1 2,198,296 - Psyr_1925 0.16 -1.0 2,198,297 + Psyr_1925 0.16 -1.6 2,198,297 + Psyr_1925 0.16 -1.0 2,198,298 - Psyr_1925 0.16 -1.0 2,198,378 + Psyr_1925 0.22 +0.0 2,198,404 - Psyr_1925 0.24 -0.7 2,198,404 - Psyr_1925 0.24 +0.3 2,198,404 - Psyr_1925 0.24 -1.7 2,198,404 - Psyr_1925 0.24 -1.6
Or see this region's nucleotide sequence