Experiment: KB with Palmatine chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1921 and Psyr_1922 are separated by 15 nucleotides Psyr_1922 and Psyr_1923 are separated by 43 nucleotides
Psyr_1921: Psyr_1921 - Alpha/beta hydrolase fold protein, at 2,194,112 to 2,195,221
_1921
Psyr_1922: Psyr_1922 - UBA/THIF-type NAD/FAD binding fold protein, at 2,195,237 to 2,196,166
_1922
Psyr_1923: Psyr_1923 - hypothetical protein, at 2,196,210 to 2,197,433
_1923
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2194.331 kb on - strand, within Psyr_1921 at 2194.331 kb on - strand, within Psyr_1921 at 2194.355 kb on - strand, within Psyr_1921 at 2194.559 kb on + strand, within Psyr_1921 at 2194.559 kb on + strand, within Psyr_1921 at 2194.560 kb on - strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.563 kb on + strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.564 kb on - strand, within Psyr_1921 at 2194.598 kb on - strand, within Psyr_1921 at 2194.631 kb on + strand, within Psyr_1921 at 2194.634 kb on + strand, within Psyr_1921 at 2194.763 kb on + strand, within Psyr_1921 at 2194.764 kb on - strand, within Psyr_1921 at 2194.797 kb on + strand, within Psyr_1921 at 2194.797 kb on + strand, within Psyr_1921 at 2194.798 kb on - strand, within Psyr_1921 at 2194.847 kb on - strand, within Psyr_1921 at 2194.847 kb on - strand, within Psyr_1921 at 2194.903 kb on + strand, within Psyr_1921 at 2194.930 kb on + strand, within Psyr_1921 at 2194.934 kb on + strand, within Psyr_1921 at 2194.934 kb on + strand, within Psyr_1921 at 2194.935 kb on - strand, within Psyr_1921 at 2194.935 kb on - strand, within Psyr_1921 at 2194.935 kb on - strand, within Psyr_1921 at 2194.935 kb on - strand, within Psyr_1921 at 2194.935 kb on - strand at 2194.956 kb on - strand, within Psyr_1921 at 2195.006 kb on + strand, within Psyr_1921 at 2195.007 kb on - strand, within Psyr_1921 at 2195.049 kb on - strand, within Psyr_1921 at 2195.151 kb on + strand at 2195.174 kb on + strand at 2195.174 kb on + strand at 2195.175 kb on - strand at 2195.176 kb on + strand at 2195.219 kb on + strand at 2195.219 kb on + strand at 2195.220 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.292 kb on - strand at 2195.445 kb on + strand, within Psyr_1922 at 2195.445 kb on + strand, within Psyr_1922 at 2195.446 kb on - strand, within Psyr_1922 at 2195.480 kb on + strand, within Psyr_1922 at 2195.533 kb on + strand, within Psyr_1922 at 2195.609 kb on - strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.610 kb on + strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.611 kb on - strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.675 kb on + strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.676 kb on - strand, within Psyr_1922 at 2195.693 kb on + strand, within Psyr_1922 at 2195.694 kb on - strand, within Psyr_1922 at 2195.717 kb on - strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.777 kb on + strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.778 kb on - strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.885 kb on + strand, within Psyr_1922 at 2195.886 kb on - strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.950 kb on + strand, within Psyr_1922 at 2195.951 kb on - strand, within Psyr_1922 at 2196.036 kb on - strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.065 kb on + strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand, within Psyr_1922 at 2196.066 kb on - strand at 2196.066 kb on - strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.068 kb on + strand, within Psyr_1922 at 2196.069 kb on - strand, within Psyr_1922 at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.085 kb on + strand at 2196.086 kb on - strand at 2196.086 kb on - strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.100 kb on + strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.101 kb on - strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.104 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.245 kb on + strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.246 kb on - strand at 2196.295 kb on + strand at 2196.296 kb on - strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.304 kb on + strand at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.405 kb on + strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.406 kb on - strand, within Psyr_1923 at 2196.459 kb on + strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.505 kb on - strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.522 kb on + strand, within Psyr_1923 at 2196.523 kb on - strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.540 kb on + strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.595 kb on - strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.604 kb on + strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.605 kb on - strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.638 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand, within Psyr_1923 at 2196.640 kb on + strand at 2196.641 kb on - strand, within Psyr_1923 at 2196.641 kb on - strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.771 kb on + strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.772 kb on - strand, within Psyr_1923 at 2196.773 kb on + strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.774 kb on - strand, within Psyr_1923 at 2196.871 kb on + strand, within Psyr_1923 at 2196.934 kb on + strand, within Psyr_1923 at 2196.935 kb on - strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.938 kb on + strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2196.939 kb on - strand, within Psyr_1923 at 2197.052 kb on + strand, within Psyr_1923 at 2197.053 kb on - strand, within Psyr_1923 at 2197.057 kb on + strand, within Psyr_1923 at 2197.094 kb on - strand, within Psyr_1923
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine chloride 10 mM remove 2,194,331 - Psyr_1921 0.20 +1.4 2,194,331 - Psyr_1921 0.20 +0.8 2,194,355 - Psyr_1921 0.22 -4.1 2,194,559 + Psyr_1921 0.40 -0.6 2,194,559 + Psyr_1921 0.40 +0.4 2,194,560 - Psyr_1921 0.40 +0.9 2,194,563 + Psyr_1921 0.41 -0.4 2,194,563 + Psyr_1921 0.41 -2.2 2,194,563 + Psyr_1921 0.41 -1.3 2,194,563 + Psyr_1921 0.41 +0.3 2,194,563 + Psyr_1921 0.41 +0.4 2,194,563 + Psyr_1921 0.41 -1.1 2,194,564 - Psyr_1921 0.41 -2.3 2,194,564 - Psyr_1921 0.41 +1.7 2,194,564 - Psyr_1921 0.41 -1.3 2,194,564 - Psyr_1921 0.41 -0.8 2,194,564 - Psyr_1921 0.41 -0.9 2,194,564 - Psyr_1921 0.41 +3.0 2,194,564 - Psyr_1921 0.41 -0.9 2,194,564 - Psyr_1921 0.41 +0.1 2,194,564 - Psyr_1921 0.41 -0.1 2,194,564 - Psyr_1921 0.41 -0.8 2,194,564 - Psyr_1921 0.41 -1.4 2,194,598 - Psyr_1921 0.44 -1.9 2,194,631 + Psyr_1921 0.47 +0.0 2,194,634 + Psyr_1921 0.47 +1.2 2,194,763 + Psyr_1921 0.59 +0.8 2,194,764 - Psyr_1921 0.59 +1.3 2,194,797 + Psyr_1921 0.62 +2.0 2,194,797 + Psyr_1921 0.62 -0.2 2,194,798 - Psyr_1921 0.62 -1.6 2,194,847 - Psyr_1921 0.66 -2.0 2,194,847 - Psyr_1921 0.66 -0.3 2,194,903 + Psyr_1921 0.71 +2.1 2,194,930 + Psyr_1921 0.74 -0.3 2,194,934 + Psyr_1921 0.74 +0.2 2,194,934 + Psyr_1921 0.74 +0.2 2,194,935 - Psyr_1921 0.74 -0.2 2,194,935 - Psyr_1921 0.74 +0.2 2,194,935 - Psyr_1921 0.74 -1.1 2,194,935 - Psyr_1921 0.74 +2.0 2,194,935 - -2.2 2,194,956 - Psyr_1921 0.76 -0.7 2,195,006 + Psyr_1921 0.81 -0.6 2,195,007 - Psyr_1921 0.81 -0.3 2,195,049 - Psyr_1921 0.84 -0.4 2,195,151 + -1.0 2,195,174 + +2.4 2,195,174 + -1.1 2,195,175 - -0.5 2,195,176 + +4.5 2,195,219 + +2.2 2,195,219 + -1.0 2,195,220 - -0.8 2,195,292 - +1.4 2,195,292 - -0.2 2,195,292 - -0.6 2,195,445 + Psyr_1922 0.22 -0.9 2,195,445 + Psyr_1922 0.22 +0.7 2,195,446 - Psyr_1922 0.22 +1.7 2,195,480 + Psyr_1922 0.26 +2.1 2,195,533 + Psyr_1922 0.32 +0.5 2,195,609 - Psyr_1922 0.40 -0.3 2,195,610 + Psyr_1922 0.40 +2.0 2,195,610 + Psyr_1922 0.40 +1.6 2,195,610 + Psyr_1922 0.40 +2.7 2,195,610 + Psyr_1922 0.40 -2.6 2,195,611 - Psyr_1922 0.40 +0.7 2,195,611 - Psyr_1922 0.40 -0.6 2,195,611 - Psyr_1922 0.40 -0.2 2,195,611 - Psyr_1922 0.40 -1.0 2,195,675 + Psyr_1922 0.47 +0.3 2,195,675 + Psyr_1922 0.47 -2.5 2,195,676 - Psyr_1922 0.47 -1.0 2,195,676 - Psyr_1922 0.47 -2.6 2,195,676 - Psyr_1922 0.47 +1.0 2,195,693 + Psyr_1922 0.49 +1.1 2,195,694 - Psyr_1922 0.49 -1.1 2,195,717 - Psyr_1922 0.52 -1.1 2,195,777 + Psyr_1922 0.58 -0.2 2,195,777 + Psyr_1922 0.58 -1.0 2,195,777 + Psyr_1922 0.58 -1.3 2,195,777 + Psyr_1922 0.58 +0.4 2,195,777 + Psyr_1922 0.58 +0.4 2,195,777 + Psyr_1922 0.58 +0.0 2,195,777 + Psyr_1922 0.58 +0.0 2,195,777 + Psyr_1922 0.58 -1.6 2,195,778 - Psyr_1922 0.58 -1.3 2,195,778 - Psyr_1922 0.58 +0.2 2,195,778 - Psyr_1922 0.58 +0.7 2,195,885 + Psyr_1922 0.70 -1.7 2,195,885 + Psyr_1922 0.70 +0.5 2,195,885 + Psyr_1922 0.70 -0.7 2,195,886 - Psyr_1922 0.70 -1.7 2,195,950 + Psyr_1922 0.77 +0.0 2,195,950 + Psyr_1922 0.77 -1.0 2,195,951 - Psyr_1922 0.77 -3.3 2,196,036 - Psyr_1922 0.86 -0.6 2,196,065 + Psyr_1922 0.89 +1.4 2,196,065 + Psyr_1922 0.89 -0.6 2,196,065 + Psyr_1922 0.89 +1.2 2,196,065 + Psyr_1922 0.89 -1.2 2,196,065 + Psyr_1922 0.89 -0.9 2,196,066 - Psyr_1922 0.89 -0.7 2,196,066 - Psyr_1922 0.89 -0.6 2,196,066 - Psyr_1922 0.89 -0.6 2,196,066 - Psyr_1922 0.89 +0.9 2,196,066 - Psyr_1922 0.89 +0.5 2,196,066 - Psyr_1922 0.89 +1.2 2,196,066 - Psyr_1922 0.89 +1.2 2,196,066 - Psyr_1922 0.89 +1.0 2,196,066 - Psyr_1922 0.89 -1.9 2,196,066 - Psyr_1922 0.89 +1.0 2,196,066 - +2.0 2,196,066 - Psyr_1922 0.89 -0.6 2,196,068 + Psyr_1922 0.89 +0.8 2,196,068 + Psyr_1922 0.89 +2.0 2,196,068 + Psyr_1922 0.89 +0.2 2,196,069 - Psyr_1922 0.89 -2.0 2,196,085 + +0.1 2,196,085 + -0.6 2,196,085 + -1.0 2,196,086 - -1.3 2,196,086 - +0.0 2,196,100 + +0.4 2,196,100 + +0.7 2,196,100 + +0.3 2,196,101 - -0.3 2,196,101 - +2.9 2,196,101 - +2.2 2,196,104 + -0.4 2,196,104 + -1.2 2,196,104 + +1.7 2,196,245 + -0.2 2,196,245 + -1.2 2,196,245 + +0.7 2,196,245 + +1.0 2,196,246 - +0.0 2,196,246 - +0.3 2,196,246 - +1.8 2,196,246 - -1.0 2,196,246 - +0.0 2,196,295 + -1.6 2,196,296 - -1.8 2,196,304 + +0.4 2,196,304 + -2.1 2,196,304 + +0.5 2,196,405 + Psyr_1923 0.16 -0.1 2,196,405 + Psyr_1923 0.16 -0.7 2,196,405 + Psyr_1923 0.16 -0.2 2,196,405 + Psyr_1923 0.16 -1.6 2,196,406 - Psyr_1923 0.16 -0.8 2,196,406 - Psyr_1923 0.16 +1.1 2,196,459 + Psyr_1923 0.20 -0.1 2,196,505 - Psyr_1923 0.24 -2.4 2,196,505 - Psyr_1923 0.24 +0.4 2,196,522 + Psyr_1923 0.25 +1.0 2,196,522 + Psyr_1923 0.25 -2.1 2,196,523 - Psyr_1923 0.26 -0.6 2,196,540 + Psyr_1923 0.27 +2.0 2,196,540 + Psyr_1923 0.27 -1.3 2,196,540 + Psyr_1923 0.27 -3.3 2,196,540 + Psyr_1923 0.27 -2.2 2,196,595 - Psyr_1923 0.31 -0.1 2,196,595 - Psyr_1923 0.31 -0.4 2,196,604 + Psyr_1923 0.32 -1.9 2,196,604 + Psyr_1923 0.32 +0.4 2,196,605 - Psyr_1923 0.32 -0.3 2,196,605 - Psyr_1923 0.32 +1.0 2,196,605 - Psyr_1923 0.32 +0.0 2,196,605 - Psyr_1923 0.32 +1.0 2,196,605 - Psyr_1923 0.32 +0.7 2,196,638 + Psyr_1923 0.35 +1.8 2,196,638 + Psyr_1923 0.35 +1.2 2,196,640 + Psyr_1923 0.35 +0.7 2,196,640 + -1.2 2,196,641 - Psyr_1923 0.35 +0.2 2,196,641 - Psyr_1923 0.35 +0.4 2,196,771 + Psyr_1923 0.46 -1.0 2,196,771 + Psyr_1923 0.46 -0.2 2,196,771 + Psyr_1923 0.46 +0.3 2,196,771 + Psyr_1923 0.46 +0.5 2,196,771 + Psyr_1923 0.46 -0.8 2,196,771 + Psyr_1923 0.46 +0.3 2,196,772 - Psyr_1923 0.46 -1.4 2,196,772 - Psyr_1923 0.46 -0.2 2,196,772 - Psyr_1923 0.46 -0.9 2,196,773 + Psyr_1923 0.46 +2.8 2,196,774 - Psyr_1923 0.46 +0.0 2,196,774 - Psyr_1923 0.46 +0.7 2,196,774 - Psyr_1923 0.46 -1.0 2,196,774 - Psyr_1923 0.46 -1.2 2,196,774 - Psyr_1923 0.46 -0.7 2,196,774 - Psyr_1923 0.46 +0.0 2,196,871 + Psyr_1923 0.54 +1.0 2,196,934 + Psyr_1923 0.59 -0.2 2,196,935 - Psyr_1923 0.59 +1.2 2,196,938 + Psyr_1923 0.59 +3.7 2,196,938 + Psyr_1923 0.59 -1.1 2,196,938 + Psyr_1923 0.59 +3.0 2,196,938 + Psyr_1923 0.59 +1.2 2,196,938 + Psyr_1923 0.59 +3.0 2,196,938 + Psyr_1923 0.59 +0.4 2,196,939 - Psyr_1923 0.60 -0.5 2,196,939 - Psyr_1923 0.60 +0.1 2,196,939 - Psyr_1923 0.60 -0.2 2,196,939 - Psyr_1923 0.60 -1.0 2,196,939 - Psyr_1923 0.60 -0.9 2,196,939 - Psyr_1923 0.60 +1.4 2,197,052 + Psyr_1923 0.69 +0.2 2,197,053 - Psyr_1923 0.69 -0.7 2,197,057 + Psyr_1923 0.69 -1.2 2,197,094 - Psyr_1923 0.72 +0.3
Or see this region's nucleotide sequence