Experiment: KB with Palmatine chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1781 and Psyr_1782 are separated by 29 nucleotides Psyr_1782 and Psyr_1783 are separated by 360 nucleotides Psyr_1783 and Psyr_1784 are separated by 55 nucleotides
Psyr_1781: Psyr_1781 - Beta-glucosidase, at 2,029,582 to 2,032,302
_1781
Psyr_1782: Psyr_1782 - conserved hypothetical protein, at 2,032,332 to 2,032,580
_1782
Psyr_1783: Psyr_1783 - CDS, at 2,032,941 to 2,033,192
_1783
Psyr_1784: Psyr_1784 - TonB-dependent siderophore receptor, at 2,033,248 to 2,035,401
_1784
Position (kb)
2032
2033
2034 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2032.033 kb on + strand at 2032.034 kb on - strand at 2032.284 kb on + strand at 2032.291 kb on + strand at 2032.291 kb on + strand at 2032.292 kb on - strand at 2032.292 kb on - strand at 2032.292 kb on - strand at 2032.546 kb on - strand, within Psyr_1782 at 2032.603 kb on + strand at 2032.603 kb on + strand at 2032.604 kb on - strand at 2032.604 kb on - strand at 2033.010 kb on + strand, within Psyr_1783 at 2033.101 kb on + strand, within Psyr_1783 at 2033.102 kb on - strand, within Psyr_1783 at 2033.130 kb on + strand, within Psyr_1783 at 2033.130 kb on + strand, within Psyr_1783 at 2033.131 kb on - strand, within Psyr_1783 at 2033.131 kb on - strand, within Psyr_1783 at 2033.131 kb on - strand, within Psyr_1783 at 2033.190 kb on + strand at 2033.258 kb on + strand at 2033.259 kb on - strand at 2033.277 kb on - strand at 2033.277 kb on - strand at 2033.339 kb on + strand at 2033.340 kb on - strand at 2033.342 kb on + strand at 2033.342 kb on + strand at 2033.343 kb on - strand at 2033.343 kb on - strand at 2033.343 kb on - strand at 2033.356 kb on + strand at 2033.356 kb on + strand at 2033.357 kb on - strand at 2033.430 kb on - strand at 2033.430 kb on - strand at 2033.480 kb on + strand, within Psyr_1784 at 2033.480 kb on + strand, within Psyr_1784 at 2033.480 kb on + strand, within Psyr_1784 at 2033.480 kb on + strand, within Psyr_1784 at 2033.480 kb on + strand, within Psyr_1784 at 2033.481 kb on - strand, within Psyr_1784 at 2033.481 kb on - strand, within Psyr_1784 at 2033.546 kb on + strand, within Psyr_1784 at 2033.630 kb on + strand, within Psyr_1784 at 2033.630 kb on + strand, within Psyr_1784 at 2033.630 kb on + strand, within Psyr_1784 at 2033.645 kb on + strand, within Psyr_1784 at 2033.719 kb on + strand, within Psyr_1784 at 2033.843 kb on + strand, within Psyr_1784 at 2033.843 kb on + strand, within Psyr_1784 at 2033.844 kb on - strand, within Psyr_1784 at 2033.867 kb on + strand, within Psyr_1784 at 2033.868 kb on - strand, within Psyr_1784 at 2033.868 kb on - strand, within Psyr_1784 at 2033.942 kb on + strand, within Psyr_1784 at 2033.942 kb on + strand, within Psyr_1784 at 2033.952 kb on - strand, within Psyr_1784 at 2033.952 kb on - strand, within Psyr_1784 at 2033.976 kb on + strand, within Psyr_1784 at 2033.976 kb on + strand, within Psyr_1784 at 2033.977 kb on - strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.978 kb on + strand, within Psyr_1784 at 2033.979 kb on - strand, within Psyr_1784 at 2034.006 kb on + strand, within Psyr_1784 at 2034.020 kb on + strand, within Psyr_1784 at 2034.020 kb on + strand, within Psyr_1784 at 2034.020 kb on + strand, within Psyr_1784 at 2034.021 kb on - strand, within Psyr_1784 at 2034.021 kb on - strand, within Psyr_1784 at 2034.021 kb on - strand, within Psyr_1784 at 2034.048 kb on - strand, within Psyr_1784 at 2034.054 kb on - strand, within Psyr_1784 at 2034.119 kb on + strand, within Psyr_1784 at 2034.120 kb on - strand, within Psyr_1784
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine chloride 10 mM remove 2,032,033 + +0.5 2,032,034 - +1.2 2,032,284 + +3.3 2,032,291 + -0.7 2,032,291 + +0.9 2,032,292 - +3.0 2,032,292 - -1.3 2,032,292 - -0.9 2,032,546 - Psyr_1782 0.86 +0.4 2,032,603 + -1.2 2,032,603 + -1.4 2,032,604 - -1.1 2,032,604 - +0.3 2,033,010 + Psyr_1783 0.27 -0.9 2,033,101 + Psyr_1783 0.63 -2.4 2,033,102 - Psyr_1783 0.64 +2.0 2,033,130 + Psyr_1783 0.75 +2.0 2,033,130 + Psyr_1783 0.75 -1.0 2,033,131 - Psyr_1783 0.75 -3.8 2,033,131 - Psyr_1783 0.75 -3.2 2,033,131 - Psyr_1783 0.75 -1.6 2,033,190 + +0.5 2,033,258 + +1.3 2,033,259 - -0.6 2,033,277 - +1.0 2,033,277 - +0.9 2,033,339 + -0.6 2,033,340 - +0.1 2,033,342 + -1.2 2,033,342 + -0.7 2,033,343 - -1.4 2,033,343 - +1.0 2,033,343 - +1.2 2,033,356 + +0.4 2,033,356 + +1.5 2,033,357 - +1.6 2,033,430 - -1.2 2,033,430 - -0.5 2,033,480 + Psyr_1784 0.11 +2.4 2,033,480 + Psyr_1784 0.11 -0.5 2,033,480 + Psyr_1784 0.11 +0.6 2,033,480 + Psyr_1784 0.11 +0.0 2,033,480 + Psyr_1784 0.11 +1.0 2,033,481 - Psyr_1784 0.11 -0.9 2,033,481 - Psyr_1784 0.11 +1.4 2,033,546 + Psyr_1784 0.14 +0.3 2,033,630 + Psyr_1784 0.18 -2.2 2,033,630 + Psyr_1784 0.18 -0.3 2,033,630 + Psyr_1784 0.18 +0.1 2,033,645 + Psyr_1784 0.18 +1.0 2,033,719 + Psyr_1784 0.22 +2.0 2,033,843 + Psyr_1784 0.28 +1.0 2,033,843 + Psyr_1784 0.28 -1.3 2,033,844 - Psyr_1784 0.28 -1.3 2,033,867 + Psyr_1784 0.29 +1.0 2,033,868 - Psyr_1784 0.29 +0.1 2,033,868 - Psyr_1784 0.29 -0.2 2,033,942 + Psyr_1784 0.32 +0.3 2,033,942 + Psyr_1784 0.32 +2.0 2,033,952 - Psyr_1784 0.33 +0.0 2,033,952 - Psyr_1784 0.33 +2.0 2,033,976 + Psyr_1784 0.34 +1.7 2,033,976 + Psyr_1784 0.34 +0.5 2,033,977 - Psyr_1784 0.34 -0.2 2,033,978 + Psyr_1784 0.34 -0.4 2,033,978 + Psyr_1784 0.34 +1.3 2,033,978 + Psyr_1784 0.34 -0.8 2,033,978 + Psyr_1784 0.34 -1.3 2,033,978 + Psyr_1784 0.34 -0.9 2,033,978 + Psyr_1784 0.34 +0.7 2,033,979 - Psyr_1784 0.34 +1.3 2,034,006 + Psyr_1784 0.35 -1.0 2,034,020 + Psyr_1784 0.36 +0.5 2,034,020 + Psyr_1784 0.36 +0.6 2,034,020 + Psyr_1784 0.36 +1.3 2,034,021 - Psyr_1784 0.36 +1.6 2,034,021 - Psyr_1784 0.36 -0.2 2,034,021 - Psyr_1784 0.36 +3.0 2,034,048 - Psyr_1784 0.37 +0.2 2,034,054 - Psyr_1784 0.37 +1.4 2,034,119 + Psyr_1784 0.40 +1.2 2,034,120 - Psyr_1784 0.40 -0.6
Or see this region's nucleotide sequence