Experiment: KB with Palmatine chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1020 and Psyr_1021 are separated by 21 nucleotides Psyr_1021 and Psyr_1022 are separated by 234 nucleotides Psyr_1022 and Psyr_1023 are separated by 151 nucleotides Psyr_1023 and Psyr_1024 are separated by 206 nucleotides
Psyr_1020: Psyr_1020 - GTP cyclohydrolase I, at 1,151,982 to 1,152,545
_1020
Psyr_1021: Psyr_1021 - Short-chain dehydrogenase/reductase SDR, at 1,152,567 to 1,153,277
_1021
Psyr_1022: Psyr_1022 - conserved hypothetical protein, at 1,153,512 to 1,154,078
_1022
Psyr_1023: Psyr_1023 - conserved hypothetical protein, at 1,154,230 to 1,154,835
_1023
Psyr_1024: Psyr_1024 - Flavodoxin/nitric oxide synthase, at 1,155,042 to 1,155,494
_1024
Position (kb) 1153
1154
1155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1152.568 kb on + strand at 1152.568 kb on + strand at 1152.569 kb on - strand at 1152.569 kb on - strand at 1152.569 kb on - strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.616 kb on + strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.617 kb on - strand at 1152.634 kb on + strand at 1152.649 kb on + strand, within Psyr_1021 at 1152.673 kb on - strand, within Psyr_1021 at 1152.710 kb on - strand, within Psyr_1021 at 1152.753 kb on + strand, within Psyr_1021 at 1152.753 kb on + strand, within Psyr_1021 at 1152.754 kb on - strand, within Psyr_1021 at 1152.754 kb on - strand, within Psyr_1021 at 1152.881 kb on + strand, within Psyr_1021 at 1152.881 kb on + strand, within Psyr_1021 at 1153.227 kb on + strand at 1153.228 kb on - strand at 1153.228 kb on - strand at 1153.228 kb on - strand at 1153.256 kb on + strand at 1153.290 kb on + strand at 1153.319 kb on + strand at 1153.320 kb on - strand at 1153.356 kb on - strand at 1153.375 kb on + strand at 1153.505 kb on + strand at 1153.506 kb on - strand at 1153.513 kb on + strand at 1153.606 kb on + strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1153.607 kb on - strand, within Psyr_1022 at 1154.072 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.081 kb on + strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.082 kb on - strand at 1154.083 kb on + strand at 1154.083 kb on + strand at 1154.083 kb on + strand at 1154.084 kb on - strand at 1154.084 kb on - strand at 1154.084 kb on - strand at 1154.183 kb on - strand at 1154.231 kb on + strand at 1154.232 kb on - strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.276 kb on + strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.277 kb on - strand at 1154.325 kb on - strand, within Psyr_1023 at 1154.325 kb on - strand, within Psyr_1023 at 1154.376 kb on + strand, within Psyr_1023 at 1154.574 kb on + strand, within Psyr_1023 at 1154.574 kb on + strand, within Psyr_1023 at 1154.749 kb on - strand, within Psyr_1023 at 1154.749 kb on - strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.758 kb on + strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.759 kb on - strand, within Psyr_1023 at 1154.998 kb on + strand at 1154.998 kb on + strand at 1154.998 kb on + strand at 1155.005 kb on + strand at 1155.005 kb on + strand at 1155.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine chloride 10 mM remove 1,152,568 + +0.5 1,152,568 + -2.4 1,152,569 - +1.8 1,152,569 - +1.9 1,152,569 - +0.2 1,152,616 + -0.4 1,152,616 + +0.8 1,152,616 + -1.8 1,152,616 + -0.8 1,152,616 + +0.2 1,152,616 + -1.7 1,152,616 + -0.9 1,152,616 + -0.3 1,152,616 + -1.7 1,152,616 + +0.5 1,152,616 + +0.8 1,152,616 + +0.4 1,152,616 + -1.1 1,152,617 - -0.2 1,152,617 - -1.6 1,152,617 - +0.3 1,152,617 - -0.9 1,152,617 - -1.1 1,152,617 - -0.4 1,152,617 - +0.2 1,152,617 - -0.1 1,152,617 - +0.2 1,152,617 - -0.9 1,152,617 - +0.2 1,152,617 - -0.9 1,152,617 - +2.9 1,152,617 - +0.8 1,152,617 - +0.3 1,152,617 - +0.5 1,152,617 - +2.1 1,152,617 - +1.8 1,152,617 - -1.5 1,152,634 + +1.9 1,152,649 + Psyr_1021 0.12 -0.8 1,152,673 - Psyr_1021 0.15 +1.5 1,152,710 - Psyr_1021 0.20 +0.4 1,152,753 + Psyr_1021 0.26 +0.5 1,152,753 + Psyr_1021 0.26 -0.2 1,152,754 - Psyr_1021 0.26 +0.5 1,152,754 - Psyr_1021 0.26 +0.7 1,152,881 + Psyr_1021 0.44 -0.2 1,152,881 + Psyr_1021 0.44 -0.6 1,153,227 + +1.0 1,153,228 - +2.4 1,153,228 - +1.3 1,153,228 - +0.5 1,153,256 + +1.9 1,153,290 + -0.3 1,153,319 + +0.4 1,153,320 - -0.0 1,153,356 - +1.2 1,153,375 + -0.4 1,153,505 + +0.5 1,153,506 - +0.1 1,153,513 + +0.6 1,153,606 + Psyr_1022 0.17 -3.1 1,153,607 - Psyr_1022 0.17 -0.4 1,153,607 - Psyr_1022 0.17 -0.6 1,153,607 - Psyr_1022 0.17 +0.3 1,154,072 + -2.4 1,154,081 + -0.5 1,154,081 + +0.8 1,154,081 + +1.5 1,154,081 + -0.3 1,154,081 + -1.3 1,154,081 + -0.3 1,154,081 + -0.0 1,154,081 + +0.1 1,154,081 + +0.6 1,154,081 + +0.0 1,154,081 + -0.4 1,154,081 + +0.1 1,154,081 + -0.2 1,154,081 + +1.1 1,154,081 + -1.4 1,154,081 + +0.6 1,154,081 + -0.3 1,154,081 + -1.5 1,154,081 + +0.9 1,154,081 + -0.4 1,154,081 + +0.3 1,154,081 + +0.7 1,154,081 + -0.6 1,154,081 + +1.4 1,154,081 + -0.1 1,154,081 + +1.2 1,154,081 + +0.4 1,154,081 + +0.6 1,154,081 + +0.5 1,154,081 + -0.1 1,154,081 + +0.7 1,154,081 + +1.6 1,154,081 + +0.4 1,154,081 + -0.2 1,154,081 + +1.1 1,154,081 + +1.1 1,154,081 + -0.1 1,154,081 + +1.2 1,154,081 + -1.5 1,154,081 + -0.8 1,154,081 + +4.2 1,154,081 + -0.3 1,154,081 + +0.4 1,154,081 + +0.9 1,154,081 + -0.2 1,154,081 + +0.7 1,154,082 - -0.7 1,154,082 - -0.6 1,154,082 - -0.2 1,154,082 - +0.8 1,154,082 - +0.1 1,154,082 - +2.1 1,154,082 - -0.0 1,154,082 - +0.7 1,154,082 - -1.4 1,154,082 - -0.8 1,154,082 - -0.7 1,154,082 - -0.7 1,154,082 - +1.1 1,154,082 - +0.4 1,154,082 - -0.4 1,154,082 - +1.9 1,154,082 - +1.4 1,154,082 - -1.0 1,154,082 - -1.9 1,154,082 - -1.7 1,154,082 - +0.1 1,154,082 - +0.6 1,154,082 - +1.1 1,154,082 - +0.7 1,154,082 - -2.5 1,154,082 - +1.1 1,154,082 - +1.3 1,154,082 - -0.1 1,154,082 - -2.2 1,154,082 - +1.1 1,154,082 - +1.1 1,154,082 - -0.5 1,154,082 - +0.9 1,154,082 - +1.5 1,154,082 - +0.1 1,154,082 - -0.6 1,154,082 - +0.1 1,154,082 - -0.5 1,154,082 - -0.8 1,154,082 - +1.1 1,154,083 + +0.6 1,154,083 + -1.8 1,154,083 + +0.3 1,154,084 - -0.9 1,154,084 - +2.6 1,154,084 - +1.3 1,154,183 - -0.4 1,154,231 + -1.5 1,154,232 - +1.9 1,154,276 + +0.7 1,154,276 + +1.7 1,154,276 + +0.1 1,154,276 + +0.9 1,154,276 + +1.1 1,154,276 + -0.6 1,154,276 + +2.0 1,154,277 - +0.7 1,154,277 - +2.4 1,154,277 - +0.7 1,154,277 - -0.2 1,154,325 - Psyr_1023 0.16 +0.8 1,154,325 - Psyr_1023 0.16 -0.4 1,154,376 + Psyr_1023 0.24 -0.4 1,154,574 + Psyr_1023 0.57 +0.1 1,154,574 + Psyr_1023 0.57 -1.2 1,154,749 - Psyr_1023 0.86 +2.7 1,154,749 - Psyr_1023 0.86 +0.5 1,154,758 + Psyr_1023 0.87 +1.7 1,154,758 + Psyr_1023 0.87 +1.1 1,154,758 + Psyr_1023 0.87 +0.7 1,154,758 + Psyr_1023 0.87 +0.5 1,154,759 - Psyr_1023 0.87 +1.0 1,154,759 - Psyr_1023 0.87 +0.9 1,154,759 - Psyr_1023 0.87 +2.0 1,154,759 - Psyr_1023 0.87 +0.8 1,154,759 - Psyr_1023 0.87 +1.4 1,154,759 - Psyr_1023 0.87 +0.5 1,154,759 - Psyr_1023 0.87 -0.4 1,154,759 - Psyr_1023 0.87 -2.5 1,154,759 - Psyr_1023 0.87 +0.1 1,154,998 + -0.0 1,154,998 + +1.5 1,154,998 + +0.6 1,155,005 + -0.3 1,155,005 + +0.2 1,155,006 - +1.3
Or see this region's nucleotide sequence