Experiment: KB with Palmatine chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0605 and Psyr_0606 overlap by 4 nucleotides Psyr_0606 and Psyr_0607 are separated by 102 nucleotides
Psyr_0605: Psyr_0605 - farnesyl-diphosphate synthase, at 695,679 to 696,566
_0605
Psyr_0606: Psyr_0606 - Exodeoxyribonuclease VII small subunit, at 696,563 to 696,808
_0606
Psyr_0607: Psyr_0607 - cystathionine beta-lyase, at 696,911 to 698,077
_0607
Position (kb) 696
697 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.617 kb on - strand at 696.684 kb on - strand, within Psyr_0606 at 696.894 kb on + strand at 696.894 kb on + strand at 697.206 kb on + strand, within Psyr_0607 at 697.206 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.375 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.649 kb on - strand, within Psyr_0607 at 697.665 kb on + strand, within Psyr_0607 at 697.666 kb on - strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.729 kb on - strand, within Psyr_0607
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine chloride 10 mM remove 695,616 + +1.4 695,616 + +1.3 695,616 + +0.2 695,616 + -0.2 695,616 + +1.5 695,616 + +0.2 695,616 + +0.1 695,617 - -1.7 696,684 - Psyr_0606 0.49 -0.1 696,894 + +2.3 696,894 + +0.8 697,206 + Psyr_0607 0.25 -1.5 697,206 + Psyr_0607 0.25 +0.4 697,320 + Psyr_0607 0.35 -0.5 697,320 + Psyr_0607 0.35 +0.2 697,343 - Psyr_0607 0.37 -1.0 697,343 - Psyr_0607 0.37 +1.2 697,375 + Psyr_0607 0.40 -0.3 697,437 + Psyr_0607 0.45 +2.7 697,437 + Psyr_0607 0.45 +0.6 697,438 - Psyr_0607 0.45 -0.6 697,438 - Psyr_0607 0.45 -0.1 697,438 - Psyr_0607 0.45 +0.8 697,438 - Psyr_0607 0.45 -0.7 697,488 + Psyr_0607 0.49 +0.7 697,488 + Psyr_0607 0.49 -0.4 697,488 + Psyr_0607 0.49 -0.6 697,488 + Psyr_0607 0.49 -0.5 697,488 + Psyr_0607 0.49 -1.9 697,488 + Psyr_0607 0.49 -0.7 697,488 + Psyr_0607 0.49 +0.4 697,489 - Psyr_0607 0.50 +1.3 697,489 - Psyr_0607 0.50 +0.1 697,489 - Psyr_0607 0.50 -1.7 697,608 + Psyr_0607 0.60 +1.7 697,608 + Psyr_0607 0.60 -0.1 697,608 + Psyr_0607 0.60 -1.3 697,608 + Psyr_0607 0.60 +0.7 697,608 + Psyr_0607 0.60 +1.1 697,609 - Psyr_0607 0.60 +0.4 697,609 - Psyr_0607 0.60 -0.1 697,609 - Psyr_0607 0.60 -1.5 697,609 - Psyr_0607 0.60 -1.2 697,609 - Psyr_0607 0.60 +3.7 697,609 - Psyr_0607 0.60 -0.4 697,649 - Psyr_0607 0.63 -0.6 697,665 + Psyr_0607 0.65 -0.3 697,666 - Psyr_0607 0.65 +1.0 697,728 + Psyr_0607 0.70 -0.7 697,728 + Psyr_0607 0.70 +1.6 697,728 + Psyr_0607 0.70 -1.1 697,729 - Psyr_0607 0.70 +1.3
Or see this region's nucleotide sequence