Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30470 and AZOBR_RS30475 overlap by 11 nucleotides AZOBR_RS30475 and AZOBR_RS30480 are separated by 4 nucleotides AZOBR_RS30480 and AZOBR_RS30485 are separated by 126 nucleotides AZOBR_RS30485 and AZOBR_RS30490 overlap by 4 nucleotides AZOBR_RS30490 and AZOBR_RS30495 are separated by 216 nucleotides
AZOBR_RS30470: AZOBR_RS30470 - phosphoenolpyruvate-protein phosphotransferase, at 184,323 to 186,095
_RS30470
AZOBR_RS30475: AZOBR_RS30475 - HPr kinase, at 186,085 to 186,426
_RS30475
AZOBR_RS30480: AZOBR_RS30480 - PTS system permease (IIAMan) nitrogen regulatory IIA protein (fragment), at 186,431 to 186,832
_RS30480
AZOBR_RS30485: AZOBR_RS30485 - nucleotide-binding protein, at 186,959 to 187,882
_RS30485
AZOBR_RS30490: AZOBR_RS30490 - aldolase, at 187,879 to 188,394
_RS30490
AZOBR_RS30495: AZOBR_RS30495 - hypothetical protein, at 188,611 to 188,913
_RS30495
Position (kb) 186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 185.974 kb on + strand at 185.975 kb on - strand at 186.161 kb on + strand, within AZOBR_RS30475 at 186.877 kb on - strand at 186.889 kb on - strand at 187.126 kb on - strand, within AZOBR_RS30485 at 187.126 kb on - strand, within AZOBR_RS30485 at 187.126 kb on - strand, within AZOBR_RS30485 at 187.126 kb on - strand, within AZOBR_RS30485 at 187.126 kb on - strand, within AZOBR_RS30485 at 187.251 kb on + strand, within AZOBR_RS30485 at 187.251 kb on + strand, within AZOBR_RS30485 at 187.275 kb on - strand, within AZOBR_RS30485 at 187.275 kb on - strand, within AZOBR_RS30485 at 187.275 kb on - strand, within AZOBR_RS30485 at 187.275 kb on - strand, within AZOBR_RS30485 at 187.750 kb on - strand, within AZOBR_RS30485 at 187.858 kb on + strand at 187.859 kb on - strand at 188.317 kb on + strand at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.744 kb on + strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495 at 188.745 kb on - strand, within AZOBR_RS30495
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 185,974 + +1.4 185,975 - +0.8 186,161 + AZOBR_RS30475 0.22 +2.4 186,877 - -1.1 186,889 - -1.0 187,126 - AZOBR_RS30485 0.18 -0.5 187,126 - AZOBR_RS30485 0.18 -1.9 187,126 - AZOBR_RS30485 0.18 -1.1 187,126 - AZOBR_RS30485 0.18 -0.3 187,126 - AZOBR_RS30485 0.18 -1.1 187,251 + AZOBR_RS30485 0.32 +0.0 187,251 + AZOBR_RS30485 0.32 -3.5 187,275 - AZOBR_RS30485 0.34 -0.1 187,275 - AZOBR_RS30485 0.34 -0.9 187,275 - AZOBR_RS30485 0.34 -1.5 187,275 - AZOBR_RS30485 0.34 -0.1 187,750 - AZOBR_RS30485 0.86 -1.8 187,858 + -0.0 187,859 - -0.6 188,317 + +1.4 188,744 + AZOBR_RS30495 0.44 -0.4 188,744 + AZOBR_RS30495 0.44 -0.1 188,744 + AZOBR_RS30495 0.44 -0.7 188,744 + AZOBR_RS30495 0.44 -1.7 188,744 + AZOBR_RS30495 0.44 +1.4 188,744 + AZOBR_RS30495 0.44 +0.4 188,744 + AZOBR_RS30495 0.44 -0.0 188,745 - AZOBR_RS30495 0.44 -1.7 188,745 - AZOBR_RS30495 0.44 -0.3 188,745 - AZOBR_RS30495 0.44 -0.8 188,745 - AZOBR_RS30495 0.44 +0.6 188,745 - AZOBR_RS30495 0.44 +0.3 188,745 - AZOBR_RS30495 0.44 +0.1
Or see this region's nucleotide sequence