Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotides AZOBR_RS00655 and AZOBR_RS00660 overlap by 1 nucleotides AZOBR_RS00660 and AZOBR_RS00665 are separated by 206 nucleotides AZOBR_RS00665 and AZOBR_RS00670 are separated by 140 nucleotides
AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481
_RS00650
AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833
_RS00655
AZOBR_RS00660: AZOBR_RS00660 - hypothetical protein, at 131,833 to 132,117
_RS00660
AZOBR_RS00665: AZOBR_RS00665 - tRNA-Ala, at 132,324 to 132,399
_RS00665
AZOBR_RS00670: AZOBR_RS00670 - membrane protein, at 132,540 to 133,586
_RS00670
Position (kb) 131
132
133 Strain fitness (log2 ratio) -1
0
1 at 131.849 kb on + strand at 132.075 kb on - strand, within AZOBR_RS00660 at 132.366 kb on + strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665 at 132.847 kb on + strand, within AZOBR_RS00670 at 132.848 kb on - strand, within AZOBR_RS00670 at 132.848 kb on - strand, within AZOBR_RS00670 at 132.913 kb on + strand, within AZOBR_RS00670 at 132.914 kb on - strand, within AZOBR_RS00670
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 131,849 + +0.5 132,075 - AZOBR_RS00660 0.85 -0.2 132,366 + AZOBR_RS00665 0.55 -0.5 132,367 - AZOBR_RS00665 0.57 -0.4 132,367 - AZOBR_RS00665 0.57 +0.2 132,847 + AZOBR_RS00670 0.29 +1.1 132,848 - AZOBR_RS00670 0.29 -0.7 132,848 - AZOBR_RS00670 0.29 -1.0 132,913 + AZOBR_RS00670 0.36 -0.1 132,914 - AZOBR_RS00670 0.36 +0.4
Or see this region's nucleotide sequence