Experiment: KB with Chrysophanol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2060 and Psyr_2061 are separated by 180 nucleotides Psyr_2061 and Psyr_2062 are separated by 112 nucleotides Psyr_2062 and Psyr_2063 are separated by 244 nucleotides
Psyr_2060: Psyr_2060 - Sec-independent protein translocase TatD, at 2,406,820 to 2,407,641
_2060
Psyr_2061: Psyr_2061 - SLT, at 2,407,822 to 2,409,243
_2061
Psyr_2062: Psyr_2062 - DoxD-like family protein, at 2,409,356 to 2,409,790
_2062
Psyr_2063: Psyr_2063 - conserved hypothetical protein, at 2,410,035 to 2,410,343
_2063
Position (kb)
2407
2408
2409
2410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2406.822 kb on - strand at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2406.925 kb on + strand, within Psyr_2060 at 2407.133 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.337 kb on + strand, within Psyr_2060 at 2407.562 kb on + strand at 2407.647 kb on + strand at 2407.989 kb on + strand, within Psyr_2061 at 2407.990 kb on - strand, within Psyr_2061 at 2408.032 kb on + strand, within Psyr_2061 at 2408.032 kb on + strand, within Psyr_2061 at 2408.033 kb on - strand, within Psyr_2061 at 2408.458 kb on + strand, within Psyr_2061 at 2408.459 kb on - strand, within Psyr_2061 at 2408.560 kb on - strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.626 kb on + strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.627 kb on - strand, within Psyr_2061 at 2408.628 kb on + strand, within Psyr_2061 at 2408.629 kb on - strand, within Psyr_2061 at 2408.668 kb on + strand, within Psyr_2061 at 2408.669 kb on - strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.680 kb on + strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.681 kb on - strand, within Psyr_2061 at 2408.965 kb on + strand, within Psyr_2061 at 2408.965 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.022 kb on + strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.023 kb on - strand, within Psyr_2061 at 2409.172 kb on + strand at 2409.172 kb on + strand at 2409.172 kb on + strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.173 kb on - strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.175 kb on + strand at 2409.176 kb on - strand at 2409.176 kb on - strand at 2409.506 kb on + strand, within Psyr_2062 at 2409.506 kb on + strand, within Psyr_2062 at 2409.506 kb on + strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.507 kb on - strand, within Psyr_2062 at 2409.916 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.923 kb on + strand at 2409.924 kb on - strand at 2409.924 kb on - strand at 2409.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM remove 2,406,822 - +0.6 2,406,925 + Psyr_2060 0.13 -1.1 2,406,925 + Psyr_2060 0.13 +1.9 2,406,925 + Psyr_2060 0.13 +1.8 2,406,925 + Psyr_2060 0.13 -0.2 2,407,133 + Psyr_2060 0.38 +1.1 2,407,337 + Psyr_2060 0.63 -0.3 2,407,337 + Psyr_2060 0.63 -1.3 2,407,337 + Psyr_2060 0.63 +2.0 2,407,562 + +2.0 2,407,647 + +1.6 2,407,989 + Psyr_2061 0.12 -1.4 2,407,990 - Psyr_2061 0.12 -0.4 2,408,032 + Psyr_2061 0.15 +0.4 2,408,032 + Psyr_2061 0.15 -0.0 2,408,033 - Psyr_2061 0.15 +1.0 2,408,458 + Psyr_2061 0.45 -1.1 2,408,459 - Psyr_2061 0.45 -1.2 2,408,560 - Psyr_2061 0.52 -0.1 2,408,626 + Psyr_2061 0.57 -1.3 2,408,626 + Psyr_2061 0.57 -1.4 2,408,626 + Psyr_2061 0.57 -0.9 2,408,626 + Psyr_2061 0.57 +1.6 2,408,627 - Psyr_2061 0.57 +2.1 2,408,627 - Psyr_2061 0.57 -0.3 2,408,627 - Psyr_2061 0.57 -0.8 2,408,627 - Psyr_2061 0.57 +0.1 2,408,627 - Psyr_2061 0.57 +0.0 2,408,628 + Psyr_2061 0.57 +0.1 2,408,629 - Psyr_2061 0.57 -1.4 2,408,668 + Psyr_2061 0.59 -0.6 2,408,669 - Psyr_2061 0.60 +1.7 2,408,680 + Psyr_2061 0.60 -0.2 2,408,680 + Psyr_2061 0.60 -0.3 2,408,680 + Psyr_2061 0.60 -0.3 2,408,680 + Psyr_2061 0.60 -1.7 2,408,680 + Psyr_2061 0.60 -0.4 2,408,681 - Psyr_2061 0.60 +1.8 2,408,681 - Psyr_2061 0.60 -1.9 2,408,681 - Psyr_2061 0.60 -0.4 2,408,681 - Psyr_2061 0.60 -0.1 2,408,681 - Psyr_2061 0.60 +0.5 2,408,681 - Psyr_2061 0.60 +0.2 2,408,681 - Psyr_2061 0.60 +0.0 2,408,681 - Psyr_2061 0.60 -0.5 2,408,965 + Psyr_2061 0.80 +1.4 2,408,965 + Psyr_2061 0.80 -0.4 2,409,022 + Psyr_2061 0.84 -1.0 2,409,022 + Psyr_2061 0.84 +1.0 2,409,022 + Psyr_2061 0.84 -1.7 2,409,023 - Psyr_2061 0.84 -0.7 2,409,023 - Psyr_2061 0.84 -0.7 2,409,023 - Psyr_2061 0.84 -0.3 2,409,172 + -3.0 2,409,172 + +0.0 2,409,172 + +1.3 2,409,173 - +0.3 2,409,173 - -0.1 2,409,173 - -1.2 2,409,173 - -0.3 2,409,175 + +0.8 2,409,175 + +0.5 2,409,175 + +0.6 2,409,175 + -0.5 2,409,175 + +0.1 2,409,176 - +0.7 2,409,176 - +1.1 2,409,506 + Psyr_2062 0.34 +1.1 2,409,506 + Psyr_2062 0.34 +0.6 2,409,506 + Psyr_2062 0.34 +1.1 2,409,507 - Psyr_2062 0.35 +1.3 2,409,507 - Psyr_2062 0.35 -0.4 2,409,507 - Psyr_2062 0.35 -1.1 2,409,507 - Psyr_2062 0.35 -0.1 2,409,507 - Psyr_2062 0.35 -0.6 2,409,916 + +0.1 2,409,923 + +0.1 2,409,923 + +2.1 2,409,923 + +0.8 2,409,923 + +0.6 2,409,923 + +0.2 2,409,924 - -0.7 2,409,924 - +1.1 2,409,964 - -0.5
Or see this region's nucleotide sequence