Experiment: BHIS with 8-hydroxy-nitroquinoline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4319 and BT4320 are separated by 25 nucleotides BT4320 and BT4321 are separated by 82 nucleotides BT4321 and BT4322 are separated by 83 nucleotides
BT4319: BT4319 - hypothetical protein (NCBI ptt file), at 5,687,867 to 5,689,357
BT4319
BT4320: BT4320 - putative zinc protease (NCBI ptt file), at 5,689,383 to 5,692,223
BT4320
BT4321: BT4321 - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI ptt file), at 5,692,306 to 5,693,106
BT4321
BT4322: BT4322 - conserved hypothetical protein, with a diacylglycerol kinase catalytic domain (NCBI ptt file), at 5,693,190 to 5,694,116
BT4322
Position (kb)
5689
5690
5691
5692
5693 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5688.405 kb on + strand, within BT4319 at 5688.405 kb on + strand, within BT4319 at 5688.415 kb on + strand, within BT4319 at 5688.422 kb on - strand, within BT4319 at 5688.553 kb on + strand, within BT4319 at 5688.556 kb on - strand, within BT4319 at 5688.556 kb on - strand, within BT4319 at 5688.680 kb on + strand, within BT4319 at 5688.681 kb on - strand, within BT4319 at 5688.690 kb on - strand, within BT4319 at 5688.733 kb on + strand, within BT4319 at 5688.914 kb on + strand, within BT4319 at 5688.929 kb on + strand, within BT4319 at 5688.930 kb on - strand, within BT4319 at 5688.961 kb on + strand, within BT4319 at 5688.961 kb on + strand, within BT4319 at 5688.985 kb on + strand, within BT4319 at 5688.986 kb on - strand, within BT4319 at 5689.166 kb on + strand, within BT4319 at 5689.173 kb on + strand, within BT4319 at 5689.196 kb on - strand, within BT4319 at 5689.353 kb on - strand at 5689.361 kb on - strand at 5689.369 kb on + strand at 5689.377 kb on - strand at 5689.405 kb on - strand at 5689.534 kb on + strand at 5689.599 kb on - strand at 5689.714 kb on - strand, within BT4320 at 5689.714 kb on - strand, within BT4320 at 5689.881 kb on - strand, within BT4320 at 5689.881 kb on - strand, within BT4320 at 5689.945 kb on + strand, within BT4320 at 5689.974 kb on - strand, within BT4320 at 5690.021 kb on - strand, within BT4320 at 5690.021 kb on - strand, within BT4320 at 5690.064 kb on + strand, within BT4320 at 5690.122 kb on - strand, within BT4320 at 5690.207 kb on + strand, within BT4320 at 5690.320 kb on + strand, within BT4320 at 5690.320 kb on + strand, within BT4320 at 5690.499 kb on - strand, within BT4320 at 5690.591 kb on + strand, within BT4320 at 5690.596 kb on - strand, within BT4320 at 5690.640 kb on - strand, within BT4320 at 5690.818 kb on - strand, within BT4320 at 5690.914 kb on + strand, within BT4320 at 5690.971 kb on + strand, within BT4320 at 5690.971 kb on + strand, within BT4320 at 5690.971 kb on + strand, within BT4320 at 5690.972 kb on - strand, within BT4320 at 5691.004 kb on + strand, within BT4320 at 5691.016 kb on - strand, within BT4320 at 5691.210 kb on - strand, within BT4320 at 5691.263 kb on + strand, within BT4320 at 5691.283 kb on + strand, within BT4320 at 5691.298 kb on + strand, within BT4320 at 5691.428 kb on - strand, within BT4320 at 5691.501 kb on + strand, within BT4320 at 5691.502 kb on - strand, within BT4320 at 5691.528 kb on - strand, within BT4320 at 5691.646 kb on + strand, within BT4320 at 5691.799 kb on - strand, within BT4320 at 5691.801 kb on + strand, within BT4320 at 5691.838 kb on + strand, within BT4320 at 5691.863 kb on - strand, within BT4320 at 5691.863 kb on - strand, within BT4320 at 5691.890 kb on + strand, within BT4320 at 5691.896 kb on + strand, within BT4320 at 5692.081 kb on + strand at 5692.082 kb on - strand at 5692.089 kb on - strand at 5692.089 kb on - strand at 5692.107 kb on - strand at 5692.111 kb on - strand at 5692.180 kb on - strand at 5692.180 kb on - strand at 5692.180 kb on - strand at 5692.180 kb on - strand at 5692.180 kb on - strand at 5692.184 kb on - strand at 5692.280 kb on + strand at 5693.127 kb on - strand at 5693.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 8-hydroxy-nitroquinoline 0.1 mM remove 5,688,405 + BT4319 0.36 -0.7 5,688,405 + BT4319 0.36 -0.4 5,688,415 + BT4319 0.37 -2.0 5,688,422 - BT4319 0.37 -1.6 5,688,553 + BT4319 0.46 -1.9 5,688,556 - BT4319 0.46 -1.0 5,688,556 - BT4319 0.46 -3.5 5,688,680 + BT4319 0.55 +0.0 5,688,681 - BT4319 0.55 -2.0 5,688,690 - BT4319 0.55 +0.0 5,688,733 + BT4319 0.58 -1.3 5,688,914 + BT4319 0.70 -1.0 5,688,929 + BT4319 0.71 -0.6 5,688,930 - BT4319 0.71 -2.0 5,688,961 + BT4319 0.73 -2.0 5,688,961 + BT4319 0.73 +0.4 5,688,985 + BT4319 0.75 -3.1 5,688,986 - BT4319 0.75 -1.0 5,689,166 + BT4319 0.87 -1.0 5,689,173 + BT4319 0.88 -1.6 5,689,196 - BT4319 0.89 -2.8 5,689,353 - -2.7 5,689,361 - -2.6 5,689,369 + +0.0 5,689,377 - -1.0 5,689,405 - +1.0 5,689,534 + -2.3 5,689,599 - -0.6 5,689,714 - BT4320 0.12 -1.6 5,689,714 - BT4320 0.12 +0.0 5,689,881 - BT4320 0.18 -1.0 5,689,881 - BT4320 0.18 +0.0 5,689,945 + BT4320 0.20 +0.0 5,689,974 - BT4320 0.21 -2.6 5,690,021 - BT4320 0.22 -1.0 5,690,021 - BT4320 0.22 -0.6 5,690,064 + BT4320 0.24 -2.3 5,690,122 - BT4320 0.26 +0.0 5,690,207 + BT4320 0.29 +0.0 5,690,320 + BT4320 0.33 -0.4 5,690,320 + BT4320 0.33 +0.0 5,690,499 - BT4320 0.39 -1.6 5,690,591 + BT4320 0.43 +0.0 5,690,596 - BT4320 0.43 -2.3 5,690,640 - BT4320 0.44 -0.2 5,690,818 - BT4320 0.51 -2.6 5,690,914 + BT4320 0.54 -2.1 5,690,971 + BT4320 0.56 -0.2 5,690,971 + BT4320 0.56 -1.0 5,690,971 + BT4320 0.56 -1.6 5,690,972 - BT4320 0.56 -1.6 5,691,004 + BT4320 0.57 -1.6 5,691,016 - BT4320 0.57 +0.4 5,691,210 - BT4320 0.64 -1.6 5,691,263 + BT4320 0.66 -1.4 5,691,283 + BT4320 0.67 -2.3 5,691,298 + BT4320 0.67 -1.3 5,691,428 - BT4320 0.72 +0.0 5,691,501 + BT4320 0.75 -1.4 5,691,502 - BT4320 0.75 -2.2 5,691,528 - BT4320 0.76 +0.0 5,691,646 + BT4320 0.80 -2.4 5,691,799 - BT4320 0.85 +0.0 5,691,801 + BT4320 0.85 +0.0 5,691,838 + BT4320 0.86 -2.3 5,691,863 - BT4320 0.87 -3.1 5,691,863 - BT4320 0.87 +0.0 5,691,890 + BT4320 0.88 -1.8 5,691,896 + BT4320 0.88 -1.6 5,692,081 + -2.0 5,692,082 - -0.7 5,692,089 - +0.0 5,692,089 - -1.0 5,692,107 - -3.4 5,692,111 - -1.0 5,692,180 - -1.1 5,692,180 - -1.0 5,692,180 - -2.9 5,692,180 - -3.3 5,692,180 - -1.0 5,692,184 - +0.0 5,692,280 + -3.1 5,693,127 - +0.9 5,693,202 - -2.6
Or see this region's nucleotide sequence