Experiment: BHIS with 8-hydroxy-nitroquinoline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3637 and BT3638 are separated by 184 nucleotides BT3638 and BT3639 overlap by 1 nucleotides BT3639 and BT3640 are separated by 9 nucleotides
BT3637: BT3637 - conserved hypothetical protein (NCBI ptt file), at 4,712,436 to 4,714,175
BT3637
BT3638: BT3638 - Na+/H+ anti-porter (NCBI ptt file), at 4,714,360 to 4,716,495
BT3638
BT3639: BT3639 - ThiF family protein, putative dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis (NCBI ptt file), at 4,716,495 to 4,717,211
BT3639
BT3640: BT3640 - lipoprotein releasing system ATP-binding protein (NCBI ptt file), at 4,717,221 to 4,717,877
BT3640
Position (kb)
4714
4715
4716
4717 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4713.381 kb on + strand, within BT3637 at 4713.381 kb on + strand, within BT3637 at 4713.381 kb on + strand, within BT3637 at 4713.382 kb on - strand, within BT3637 at 4713.382 kb on - strand, within BT3637 at 4713.383 kb on + strand, within BT3637 at 4713.383 kb on + strand, within BT3637 at 4713.384 kb on - strand, within BT3637 at 4713.461 kb on + strand, within BT3637 at 4713.495 kb on + strand, within BT3637 at 4713.501 kb on - strand, within BT3637 at 4713.527 kb on + strand, within BT3637 at 4713.566 kb on + strand, within BT3637 at 4713.566 kb on + strand, within BT3637 at 4713.662 kb on + strand, within BT3637 at 4713.663 kb on - strand, within BT3637 at 4713.673 kb on + strand, within BT3637 at 4713.755 kb on + strand, within BT3637 at 4713.806 kb on - strand, within BT3637 at 4713.809 kb on + strand, within BT3637 at 4713.831 kb on + strand, within BT3637 at 4713.841 kb on + strand, within BT3637 at 4713.913 kb on + strand, within BT3637 at 4713.942 kb on - strand, within BT3637 at 4713.992 kb on + strand, within BT3637 at 4714.001 kb on - strand, within BT3637 at 4714.001 kb on - strand, within BT3637 at 4714.057 kb on - strand at 4714.115 kb on - strand at 4714.267 kb on + strand at 4714.268 kb on - strand at 4714.272 kb on - strand at 4714.291 kb on - strand at 4714.291 kb on - strand at 4714.359 kb on + strand at 4714.359 kb on + strand at 4714.479 kb on + strand at 4714.582 kb on + strand, within BT3638 at 4714.582 kb on + strand, within BT3638 at 4714.582 kb on + strand, within BT3638 at 4714.584 kb on + strand, within BT3638 at 4714.665 kb on + strand, within BT3638 at 4714.692 kb on + strand, within BT3638 at 4714.704 kb on + strand, within BT3638 at 4714.752 kb on + strand, within BT3638 at 4714.756 kb on + strand, within BT3638 at 4714.756 kb on + strand, within BT3638 at 4714.785 kb on + strand, within BT3638 at 4714.851 kb on + strand, within BT3638 at 4715.011 kb on + strand, within BT3638 at 4715.294 kb on + strand, within BT3638 at 4715.341 kb on + strand, within BT3638 at 4715.346 kb on + strand, within BT3638 at 4715.474 kb on + strand, within BT3638 at 4715.718 kb on + strand, within BT3638 at 4715.727 kb on + strand, within BT3638 at 4715.727 kb on + strand, within BT3638 at 4715.727 kb on + strand, within BT3638 at 4715.855 kb on + strand, within BT3638 at 4715.859 kb on + strand, within BT3638 at 4715.880 kb on + strand, within BT3638 at 4716.009 kb on + strand, within BT3638 at 4716.253 kb on + strand, within BT3638 at 4716.253 kb on + strand, within BT3638 at 4716.574 kb on + strand, within BT3639 at 4716.655 kb on + strand, within BT3639 at 4716.917 kb on + strand, within BT3639 at 4716.996 kb on + strand, within BT3639 at 4717.157 kb on + strand at 4717.199 kb on + strand at 4717.217 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 8-hydroxy-nitroquinoline 0.1 mM remove 4,713,381 + BT3637 0.54 -1.4 4,713,381 + BT3637 0.54 +0.3 4,713,381 + BT3637 0.54 -0.1 4,713,382 - BT3637 0.54 -0.1 4,713,382 - BT3637 0.54 +0.2 4,713,383 + BT3637 0.54 -2.2 4,713,383 + BT3637 0.54 +0.3 4,713,384 - BT3637 0.54 +0.9 4,713,461 + BT3637 0.59 -0.8 4,713,495 + BT3637 0.61 -2.0 4,713,501 - BT3637 0.61 -1.2 4,713,527 + BT3637 0.63 -2.7 4,713,566 + BT3637 0.65 -0.1 4,713,566 + BT3637 0.65 -1.1 4,713,662 + BT3637 0.70 -1.2 4,713,663 - BT3637 0.71 -1.2 4,713,673 + BT3637 0.71 -1.9 4,713,755 + BT3637 0.76 -1.4 4,713,806 - BT3637 0.79 +2.2 4,713,809 + BT3637 0.79 -1.7 4,713,831 + BT3637 0.80 -0.1 4,713,841 + BT3637 0.81 -2.1 4,713,913 + BT3637 0.85 +1.5 4,713,942 - BT3637 0.87 -0.8 4,713,992 + BT3637 0.89 -1.1 4,714,001 - BT3637 0.90 -3.1 4,714,001 - BT3637 0.90 -0.1 4,714,057 - -1.7 4,714,115 - -1.9 4,714,267 + -0.1 4,714,268 - -0.4 4,714,272 - -0.1 4,714,291 - -1.7 4,714,291 - -0.7 4,714,359 + -1.5 4,714,359 + +1.9 4,714,479 + -1.2 4,714,582 + BT3638 0.10 -0.1 4,714,582 + BT3638 0.10 +0.2 4,714,582 + BT3638 0.10 -0.1 4,714,584 + BT3638 0.10 +0.5 4,714,665 + BT3638 0.14 -1.2 4,714,692 + BT3638 0.16 -0.8 4,714,704 + BT3638 0.16 -1.7 4,714,752 + BT3638 0.18 -0.6 4,714,756 + BT3638 0.19 -0.1 4,714,756 + BT3638 0.19 -1.2 4,714,785 + BT3638 0.20 -1.1 4,714,851 + BT3638 0.23 -0.1 4,715,011 + BT3638 0.30 -2.3 4,715,294 + BT3638 0.44 +1.1 4,715,341 + BT3638 0.46 -0.1 4,715,346 + BT3638 0.46 +1.9 4,715,474 + BT3638 0.52 +0.5 4,715,718 + BT3638 0.64 -1.9 4,715,727 + BT3638 0.64 -1.7 4,715,727 + BT3638 0.64 -1.7 4,715,727 + BT3638 0.64 -0.9 4,715,855 + BT3638 0.70 +0.3 4,715,859 + BT3638 0.70 -1.7 4,715,880 + BT3638 0.71 -0.7 4,716,009 + BT3638 0.77 -0.1 4,716,253 + BT3638 0.89 -0.4 4,716,253 + BT3638 0.89 -1.7 4,716,574 + BT3639 0.11 -1.7 4,716,655 + BT3639 0.22 -0.8 4,716,917 + BT3639 0.59 -2.6 4,716,996 + BT3639 0.70 -2.0 4,717,157 + +0.3 4,717,199 + -1.1 4,717,217 + +0.2
Or see this region's nucleotide sequence