Experiment: BHIS with 8-hydroxy-nitroquinoline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2243 and BT2244 are separated by 5 nucleotides BT2244 and BT2245 are separated by 6 nucleotides
BT2243: BT2243 - conserved hypothetical protein (NCBI ptt file), at 2,810,207 to 2,812,687
BT2243
BT2244: BT2244 - hypothetical protein (NCBI ptt file), at 2,812,693 to 2,813,778
BT2244
BT2245: BT2245 - hypothetical protein (NCBI ptt file), at 2,813,785 to 2,814,744
BT2245
Position (kb)
2812
2813
2814 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2811.726 kb on + strand, within BT2243 at 2811.766 kb on - strand, within BT2243 at 2811.836 kb on + strand, within BT2243 at 2811.855 kb on - strand, within BT2243 at 2811.861 kb on + strand, within BT2243 at 2811.861 kb on + strand at 2811.861 kb on + strand, within BT2243 at 2811.861 kb on + strand, within BT2243 at 2811.862 kb on - strand, within BT2243 at 2812.064 kb on + strand, within BT2243 at 2812.065 kb on - strand, within BT2243 at 2812.065 kb on - strand, within BT2243 at 2812.065 kb on - strand, within BT2243 at 2812.065 kb on - strand, within BT2243 at 2812.116 kb on + strand, within BT2243 at 2812.145 kb on + strand, within BT2243 at 2812.145 kb on + strand, within BT2243 at 2812.225 kb on + strand, within BT2243 at 2812.237 kb on + strand, within BT2243 at 2812.237 kb on + strand, within BT2243 at 2812.238 kb on - strand, within BT2243 at 2812.238 kb on - strand, within BT2243 at 2812.246 kb on + strand, within BT2243 at 2812.251 kb on - strand, within BT2243 at 2812.251 kb on - strand, within BT2243 at 2812.355 kb on - strand, within BT2243 at 2812.355 kb on - strand, within BT2243 at 2812.355 kb on - strand, within BT2243 at 2812.376 kb on + strand, within BT2243 at 2812.410 kb on - strand, within BT2243 at 2812.426 kb on + strand, within BT2243 at 2812.601 kb on - strand at 2812.638 kb on - strand at 2812.642 kb on + strand at 2812.643 kb on - strand at 2812.670 kb on - strand at 2812.686 kb on - strand at 2812.773 kb on - strand at 2812.775 kb on - strand at 2812.875 kb on + strand, within BT2244 at 2812.876 kb on - strand, within BT2244 at 2812.986 kb on + strand, within BT2244 at 2812.987 kb on - strand, within BT2244 at 2812.990 kb on + strand, within BT2244 at 2813.001 kb on - strand, within BT2244 at 2813.001 kb on - strand, within BT2244 at 2813.128 kb on + strand, within BT2244 at 2813.142 kb on - strand, within BT2244 at 2813.207 kb on + strand, within BT2244 at 2813.208 kb on - strand, within BT2244 at 2813.246 kb on - strand, within BT2244 at 2813.255 kb on + strand, within BT2244 at 2813.256 kb on - strand, within BT2244 at 2813.261 kb on - strand, within BT2244 at 2813.261 kb on - strand, within BT2244 at 2813.264 kb on - strand, within BT2244 at 2813.264 kb on - strand, within BT2244 at 2813.376 kb on - strand, within BT2244 at 2813.418 kb on + strand, within BT2244 at 2813.490 kb on + strand, within BT2244 at 2813.490 kb on + strand, within BT2244 at 2813.592 kb on - strand, within BT2244 at 2813.653 kb on - strand, within BT2244 at 2813.653 kb on - strand, within BT2244 at 2813.692 kb on - strand at 2813.878 kb on + strand at 2813.958 kb on + strand, within BT2245 at 2814.048 kb on - strand at 2814.141 kb on + strand, within BT2245 at 2814.141 kb on + strand, within BT2245 at 2814.142 kb on - strand, within BT2245 at 2814.209 kb on + strand, within BT2245 at 2814.316 kb on + strand, within BT2245 at 2814.466 kb on + strand, within BT2245 at 2814.544 kb on + strand, within BT2245 at 2814.545 kb on - strand, within BT2245 at 2814.601 kb on - strand, within BT2245 at 2814.601 kb on - strand, within BT2245 at 2814.726 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 8-hydroxy-nitroquinoline 0.1 mM remove 2,811,726 + BT2243 0.61 -1.6 2,811,766 - BT2243 0.63 +0.1 2,811,836 + BT2243 0.66 -1.0 2,811,855 - BT2243 0.66 -1.3 2,811,861 + BT2243 0.67 -2.0 2,811,861 + -0.0 2,811,861 + BT2243 0.67 -0.0 2,811,861 + BT2243 0.67 -2.4 2,811,862 - BT2243 0.67 -1.8 2,812,064 + BT2243 0.75 -0.0 2,812,065 - BT2243 0.75 -1.8 2,812,065 - BT2243 0.75 -0.5 2,812,065 - BT2243 0.75 -1.6 2,812,065 - BT2243 0.75 -0.0 2,812,116 + BT2243 0.77 -1.1 2,812,145 + BT2243 0.78 -0.3 2,812,145 + BT2243 0.78 -0.0 2,812,225 + BT2243 0.81 -1.2 2,812,237 + BT2243 0.82 -1.6 2,812,237 + BT2243 0.82 -2.0 2,812,238 - BT2243 0.82 -0.6 2,812,238 - BT2243 0.82 -1.8 2,812,246 + BT2243 0.82 -1.3 2,812,251 - BT2243 0.82 +0.4 2,812,251 - BT2243 0.82 +1.3 2,812,355 - BT2243 0.87 -2.3 2,812,355 - BT2243 0.87 -0.6 2,812,355 - BT2243 0.87 -0.0 2,812,376 + BT2243 0.87 -1.4 2,812,410 - BT2243 0.89 +0.3 2,812,426 + BT2243 0.89 +1.8 2,812,601 - -1.4 2,812,638 - -0.0 2,812,642 + -1.0 2,812,643 - -0.6 2,812,670 - -1.5 2,812,686 - -0.0 2,812,773 - -2.8 2,812,775 - -2.2 2,812,875 + BT2244 0.17 +1.6 2,812,876 - BT2244 0.17 -1.7 2,812,986 + BT2244 0.27 -1.7 2,812,987 - BT2244 0.27 -2.6 2,812,990 + BT2244 0.27 -0.6 2,813,001 - BT2244 0.28 -1.0 2,813,001 - BT2244 0.28 -0.0 2,813,128 + BT2244 0.40 -1.4 2,813,142 - BT2244 0.41 -2.8 2,813,207 + BT2244 0.47 -2.6 2,813,208 - BT2244 0.47 -0.8 2,813,246 - BT2244 0.51 -1.0 2,813,255 + BT2244 0.52 -1.6 2,813,256 - BT2244 0.52 -2.5 2,813,261 - BT2244 0.52 -1.4 2,813,261 - BT2244 0.52 -0.0 2,813,264 - BT2244 0.53 -0.0 2,813,264 - BT2244 0.53 -2.7 2,813,376 - BT2244 0.63 -0.6 2,813,418 + BT2244 0.67 -0.8 2,813,490 + BT2244 0.73 -1.0 2,813,490 + BT2244 0.73 -2.5 2,813,592 - BT2244 0.83 +0.5 2,813,653 - BT2244 0.88 -1.3 2,813,653 - BT2244 0.88 +1.0 2,813,692 - -0.0 2,813,878 + +1.2 2,813,958 + BT2245 0.18 -1.3 2,814,048 - +1.0 2,814,141 + BT2245 0.37 -2.4 2,814,141 + BT2245 0.37 -3.2 2,814,142 - BT2245 0.37 -0.2 2,814,209 + BT2245 0.44 +1.0 2,814,316 + BT2245 0.55 -2.1 2,814,466 + BT2245 0.71 -2.0 2,814,544 + BT2245 0.79 -0.0 2,814,545 - BT2245 0.79 -1.4 2,814,601 - BT2245 0.85 -0.5 2,814,601 - BT2245 0.85 -1.6 2,814,726 + -1.0
Or see this region's nucleotide sequence