Experiment: BHIS with 8-hydroxy-nitroquinoline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0584 and BT0585 are separated by 19 nucleotides BT0585 and BT0586 are separated by 116 nucleotides BT0586 and BT0587 are separated by 145 nucleotides
BT0584: BT0584 - putative outer membrane protein (NCBI ptt file), at 721,668 to 722,666
BT0584
BT0585: BT0585 - conserved hypothetical protein (NCBI ptt file), at 722,686 to 723,195
BT0585
BT0586: BT0586 - hypothetical protein (NCBI ptt file), at 723,312 to 724,004
BT0586
BT0587: BT0587 - prolyl oligopeptidase family protein (NCBI ptt file), at 724,150 to 726,249
BT0587
Position (kb) 722
723
724 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 721.910 kb on - strand, within BT0584 at 722.208 kb on + strand, within BT0584 at 722.345 kb on + strand, within BT0584 at 722.345 kb on + strand, within BT0584 at 722.436 kb on - strand, within BT0584 at 722.465 kb on + strand, within BT0584 at 722.516 kb on - strand, within BT0584 at 722.582 kb on - strand at 722.785 kb on - strand, within BT0585 at 722.869 kb on + strand, within BT0585 at 723.067 kb on + strand, within BT0585 at 723.164 kb on + strand at 723.234 kb on - strand at 723.234 kb on - strand at 723.247 kb on - strand at 723.247 kb on - strand at 723.247 kb on - strand at 723.287 kb on - strand at 723.330 kb on - strand at 723.353 kb on + strand at 723.407 kb on - strand, within BT0586 at 723.473 kb on - strand, within BT0586 at 723.523 kb on + strand, within BT0586 at 723.523 kb on + strand, within BT0586 at 723.523 kb on + strand, within BT0586 at 723.525 kb on + strand, within BT0586 at 723.526 kb on - strand, within BT0586 at 723.565 kb on + strand, within BT0586 at 723.746 kb on + strand, within BT0586 at 723.751 kb on + strand, within BT0586 at 723.769 kb on - strand, within BT0586 at 723.769 kb on - strand, within BT0586 at 723.852 kb on + strand, within BT0586 at 723.916 kb on + strand, within BT0586 at 724.025 kb on - strand at 724.026 kb on + strand at 724.028 kb on + strand at 724.081 kb on - strand at 724.129 kb on + strand at 724.130 kb on - strand at 724.140 kb on + strand at 724.141 kb on - strand at 724.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with 8-hydroxy-nitroquinoline 0.1 mM remove 721,910 - BT0584 0.24 -3.0 722,208 + BT0584 0.54 +0.2 722,345 + BT0584 0.68 -1.4 722,345 + BT0584 0.68 -0.5 722,436 - BT0584 0.77 +0.6 722,465 + BT0584 0.80 +0.2 722,516 - BT0584 0.85 +0.2 722,582 - -1.4 722,785 - BT0585 0.19 -2.9 722,869 + BT0585 0.36 -2.2 723,067 + BT0585 0.75 -1.4 723,164 + +1.1 723,234 - -0.4 723,234 - -0.9 723,247 - -1.0 723,247 - +1.1 723,247 - +0.1 723,287 - -0.9 723,330 - -1.0 723,353 + -0.9 723,407 - BT0586 0.14 -0.2 723,473 - BT0586 0.23 -0.9 723,523 + BT0586 0.30 +0.1 723,523 + BT0586 0.30 +0.1 723,523 + BT0586 0.30 +1.1 723,525 + BT0586 0.31 -2.4 723,526 - BT0586 0.31 -0.9 723,565 + BT0586 0.37 -0.9 723,746 + BT0586 0.63 -1.6 723,751 + BT0586 0.63 +0.1 723,769 - BT0586 0.66 -1.9 723,769 - BT0586 0.66 +0.1 723,852 + BT0586 0.78 -0.9 723,916 + BT0586 0.87 +0.1 724,025 - -2.2 724,026 + -0.9 724,028 + -1.3 724,081 - -1.8 724,129 + -0.9 724,130 - +0.1 724,140 + -3.6 724,141 - -0.9 724,177 - -1.3
Or see this region's nucleotide sequence