Experiment: Varel_Bryant_medium_Glucose with Furaltadone 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4359 and BT4360 are separated by 451 nucleotides BT4360 and BT4361 are separated by 61 nucleotides BT4361 and BT4362 are separated by 121 nucleotides
BT4359: BT4359 - alpha-N-acetylglucosaminidase precursor (NCBI ptt file), at 5,747,129 to 5,749,363
BT4359
BT4360: BT4360 - hypothetical protein (NCBI ptt file), at 5,749,815 to 5,750,261
BT4360
BT4361: BT4361 - hypothetical protein (NCBI ptt file), at 5,750,323 to 5,751,432
BT4361
BT4362: BT4362 - preprotein translocase SecA subunit (NCBI ptt file), at 5,751,554 to 5,754,874
BT4362
Position (kb) 5750
5751
5752 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5749.374 kb on + strand at 5749.396 kb on - strand at 5749.619 kb on - strand at 5749.728 kb on + strand at 5749.775 kb on + strand at 5749.895 kb on + strand, within BT4360 at 5749.896 kb on - strand, within BT4360 at 5749.951 kb on - strand, within BT4360 at 5749.971 kb on + strand, within BT4360 at 5749.971 kb on + strand, within BT4360 at 5749.972 kb on - strand, within BT4360 at 5749.978 kb on - strand, within BT4360 at 5749.978 kb on - strand, within BT4360 at 5750.038 kb on - strand, within BT4360 at 5750.065 kb on + strand, within BT4360 at 5750.066 kb on - strand, within BT4360 at 5750.119 kb on + strand, within BT4360 at 5750.119 kb on + strand, within BT4360 at 5750.124 kb on - strand, within BT4360 at 5750.193 kb on + strand, within BT4360 at 5750.193 kb on + strand, within BT4360 at 5750.197 kb on + strand at 5750.198 kb on - strand, within BT4360 at 5750.262 kb on - strand at 5750.262 kb on - strand at 5750.262 kb on - strand at 5750.290 kb on + strand at 5750.305 kb on - strand at 5750.337 kb on + strand at 5750.379 kb on + strand at 5750.412 kb on + strand at 5750.412 kb on + strand at 5750.412 kb on + strand at 5750.552 kb on + strand, within BT4361 at 5750.609 kb on + strand, within BT4361 at 5750.610 kb on - strand, within BT4361 at 5750.706 kb on - strand, within BT4361 at 5750.706 kb on - strand, within BT4361 at 5750.797 kb on + strand, within BT4361 at 5750.867 kb on - strand, within BT4361 at 5750.872 kb on - strand, within BT4361 at 5750.901 kb on - strand, within BT4361 at 5750.926 kb on + strand, within BT4361 at 5750.966 kb on + strand, within BT4361 at 5750.966 kb on + strand, within BT4361 at 5750.967 kb on - strand, within BT4361 at 5751.015 kb on + strand, within BT4361 at 5751.015 kb on + strand, within BT4361 at 5751.016 kb on - strand, within BT4361 at 5751.180 kb on + strand, within BT4361 at 5751.186 kb on + strand, within BT4361 at 5751.186 kb on + strand, within BT4361 at 5751.187 kb on - strand, within BT4361 at 5751.197 kb on + strand, within BT4361 at 5751.345 kb on - strand at 5751.395 kb on - strand at 5751.441 kb on + strand at 5751.534 kb on - strand at 5751.540 kb on - strand at 5751.540 kb on - strand at 5751.556 kb on - strand at 5751.556 kb on - strand at 5751.730 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Furaltadone 0.05 mM remove 5,749,374 + -2.0 5,749,396 - +0.1 5,749,619 - +0.1 5,749,728 + -0.9 5,749,775 + -1.1 5,749,895 + BT4360 0.18 -0.2 5,749,896 - BT4360 0.18 -0.9 5,749,951 - BT4360 0.30 +0.3 5,749,971 + BT4360 0.35 +0.1 5,749,971 + BT4360 0.35 -1.9 5,749,972 - BT4360 0.35 -0.5 5,749,978 - BT4360 0.36 -1.5 5,749,978 - BT4360 0.36 +0.5 5,750,038 - BT4360 0.50 -0.4 5,750,065 + BT4360 0.56 -1.1 5,750,066 - BT4360 0.56 -0.1 5,750,119 + BT4360 0.68 +2.1 5,750,119 + BT4360 0.68 -1.7 5,750,124 - BT4360 0.69 -1.7 5,750,193 + BT4360 0.85 -2.8 5,750,193 + BT4360 0.85 -0.3 5,750,197 + +0.1 5,750,198 - BT4360 0.86 -1.2 5,750,262 - +0.2 5,750,262 - -2.2 5,750,262 - -1.1 5,750,290 + -1.4 5,750,305 - -1.3 5,750,337 + -1.7 5,750,379 + -1.5 5,750,412 + +1.1 5,750,412 + -0.5 5,750,412 + +0.1 5,750,552 + BT4361 0.21 -0.5 5,750,609 + BT4361 0.26 -1.5 5,750,610 - BT4361 0.26 -2.3 5,750,706 - BT4361 0.35 +0.3 5,750,706 - BT4361 0.35 -0.9 5,750,797 + BT4361 0.43 -3.5 5,750,867 - BT4361 0.49 -1.8 5,750,872 - BT4361 0.49 -2.4 5,750,901 - BT4361 0.52 -2.3 5,750,926 + BT4361 0.54 -0.5 5,750,966 + BT4361 0.58 +1.4 5,750,966 + BT4361 0.58 -0.9 5,750,967 - BT4361 0.58 -1.2 5,751,015 + BT4361 0.62 -2.3 5,751,015 + BT4361 0.62 -2.9 5,751,016 - BT4361 0.62 -1.9 5,751,180 + BT4361 0.77 -0.7 5,751,186 + BT4361 0.78 -2.8 5,751,186 + BT4361 0.78 +0.1 5,751,187 - BT4361 0.78 -2.9 5,751,197 + BT4361 0.79 +0.1 5,751,345 - -2.5 5,751,395 - -0.8 5,751,441 + -1.1 5,751,534 - -1.8 5,751,540 - +1.3 5,751,540 - -0.4 5,751,556 - -0.5 5,751,556 - +1.1 5,751,730 - +0.6
Or see this region's nucleotide sequence