Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS10455

Experiment: Octanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS10445 and ABZR87_RS10450 are separated by 17 nucleotidesABZR87_RS10450 and ABZR87_RS10455 are separated by 91 nucleotidesABZR87_RS10455 and ABZR87_RS10460 are separated by 23 nucleotidesABZR87_RS10460 and ABZR87_RS10465 are separated by 91 nucleotides ABZR87_RS10445: ABZR87_RS10445 - peptidase, at 2,213,866 to 2,214,768 _RS10445 ABZR87_RS10450: ABZR87_RS10450 - alpha/beta hydrolase, at 2,214,786 to 2,215,607 _RS10450 ABZR87_RS10455: ABZR87_RS10455 - CTP synthase, at 2,215,699 to 2,217,363 _RS10455 ABZR87_RS10460: ABZR87_RS10460 - 3-deoxy-8-phosphooctulonate synthase, at 2,217,387 to 2,218,241 _RS10460 ABZR87_RS10465: ABZR87_RS10465 - DUF1330 domain-containing protein, at 2,218,333 to 2,218,623 _RS10465 Position (kb) 2215 2216 2217 2218Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2214.837 kb on + strandat 2214.837 kb on + strandat 2214.838 kb on - strandat 2214.838 kb on - strandat 2215.267 kb on - strand, within ABZR87_RS10450at 2215.267 kb on - strand, within ABZR87_RS10450at 2215.267 kb on - strand, within ABZR87_RS10450at 2215.338 kb on + strand, within ABZR87_RS10450at 2215.339 kb on - strand, within ABZR87_RS10450at 2215.347 kb on + strand, within ABZR87_RS10450at 2215.348 kb on - strand, within ABZR87_RS10450at 2215.512 kb on + strand, within ABZR87_RS10450at 2215.513 kb on - strand, within ABZR87_RS10450at 2215.605 kb on + strandat 2215.668 kb on + strandat 2215.668 kb on + strandat 2215.668 kb on + strandat 2215.668 kb on + strandat 2215.669 kb on - strandat 2215.669 kb on - strandat 2218.345 kb on + strandat 2218.346 kb on - strandat 2218.346 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Octanoic (C)
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2,214,837 + +2.2
2,214,837 + -1.1
2,214,838 - -0.4
2,214,838 - -0.8
2,215,267 - ABZR87_RS10450 0.59 +0.2
2,215,267 - ABZR87_RS10450 0.59 +0.2
2,215,267 - ABZR87_RS10450 0.59 +0.6
2,215,338 + ABZR87_RS10450 0.67 +0.9
2,215,339 - ABZR87_RS10450 0.67 +0.7
2,215,347 + ABZR87_RS10450 0.68 -1.2
2,215,348 - ABZR87_RS10450 0.68 +2.9
2,215,512 + ABZR87_RS10450 0.88 +3.2
2,215,513 - ABZR87_RS10450 0.88 -1.6
2,215,605 + -0.9
2,215,668 + +1.4
2,215,668 + +1.2
2,215,668 + +1.4
2,215,668 + +0.1
2,215,669 - -2.1
2,215,669 - -0.8
2,218,345 + +0.7
2,218,346 - -0.5
2,218,346 - +0.2

Or see this region's nucleotide sequence