Experiment: R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_003890 and ABIE53_003891 are separated by 49 nucleotides ABIE53_003891 and ABIE53_003892 are separated by 4 nucleotides ABIE53_003892 and ABIE53_003893 are separated by 336 nucleotides
ABIE53_003890: ABIE53_003890 - 5-methylthioribose kinase, at 28,661 to 29,929
_003890
ABIE53_003891: ABIE53_003891 - DNA-binding GntR family transcriptional regulator, at 29,979 to 30,440
_003891
ABIE53_003892: ABIE53_003892 - DNA-binding FadR family transcriptional regulator, at 30,445 to 30,699
_003892
ABIE53_003893: ABIE53_003893 - CRP/FNR family cyclic AMP-dependent transcriptional regulator, at 31,036 to 31,800
_003893
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 29.024 kb on + strand, within ABIE53_003890 at 29.110 kb on - strand, within ABIE53_003890 at 29.110 kb on - strand, within ABIE53_003890 at 29.353 kb on - strand, within ABIE53_003890 at 29.353 kb on - strand, within ABIE53_003890 at 29.356 kb on + strand, within ABIE53_003890 at 29.506 kb on - strand, within ABIE53_003890 at 29.506 kb on - strand, within ABIE53_003890 at 29.506 kb on - strand, within ABIE53_003890 at 29.625 kb on + strand, within ABIE53_003890 at 29.626 kb on - strand, within ABIE53_003890 at 29.626 kb on - strand, within ABIE53_003890 at 29.626 kb on - strand, within ABIE53_003890 at 29.797 kb on - strand, within ABIE53_003890 at 29.802 kb on + strand, within ABIE53_003890 at 29.803 kb on - strand at 29.803 kb on - strand at 29.890 kb on - strand at 29.912 kb on - strand at 30.025 kb on + strand at 30.026 kb on - strand, within ABIE53_003891 at 30.026 kb on - strand, within ABIE53_003891 at 30.029 kb on - strand, within ABIE53_003891 at 30.038 kb on + strand, within ABIE53_003891 at 30.039 kb on - strand, within ABIE53_003891 at 30.039 kb on - strand, within ABIE53_003891 at 30.164 kb on + strand, within ABIE53_003891 at 30.165 kb on - strand, within ABIE53_003891 at 30.165 kb on - strand, within ABIE53_003891 at 30.269 kb on - strand, within ABIE53_003891 at 30.729 kb on - strand at 30.730 kb on + strand at 30.730 kb on + strand at 30.730 kb on + strand at 30.731 kb on - strand at 30.942 kb on + strand at 30.943 kb on - strand at 30.955 kb on + strand at 30.956 kb on - strand at 30.984 kb on - strand at 31.023 kb on + strand at 31.131 kb on + strand, within ABIE53_003893 at 31.132 kb on - strand, within ABIE53_003893 at 31.147 kb on + strand, within ABIE53_003893 at 31.148 kb on - strand, within ABIE53_003893 at 31.178 kb on - strand, within ABIE53_003893 at 31.178 kb on - strand, within ABIE53_003893 at 31.200 kb on - strand, within ABIE53_003893 at 31.208 kb on - strand, within ABIE53_003893 at 31.300 kb on + strand, within ABIE53_003893 at 31.342 kb on + strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.343 kb on - strand, within ABIE53_003893 at 31.417 kb on + strand, within ABIE53_003893 at 31.417 kb on + strand, within ABIE53_003893
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM remove 29,024 + ABIE53_003890 0.29 -2.0 29,110 - ABIE53_003890 0.35 +0.2 29,110 - ABIE53_003890 0.35 -0.5 29,353 - ABIE53_003890 0.55 -0.3 29,353 - ABIE53_003890 0.55 -1.4 29,356 + ABIE53_003890 0.55 +0.0 29,506 - ABIE53_003890 0.67 -1.1 29,506 - ABIE53_003890 0.67 -2.2 29,506 - ABIE53_003890 0.67 -1.5 29,625 + ABIE53_003890 0.76 -0.4 29,626 - ABIE53_003890 0.76 +0.8 29,626 - ABIE53_003890 0.76 +0.8 29,626 - ABIE53_003890 0.76 -0.4 29,797 - ABIE53_003890 0.90 -0.2 29,802 + ABIE53_003890 0.90 -0.8 29,803 - -0.8 29,803 - +1.8 29,890 - +2.8 29,912 - -2.4 30,025 + +1.2 30,026 - ABIE53_003891 0.10 -0.5 30,026 - ABIE53_003891 0.10 -0.1 30,029 - ABIE53_003891 0.11 +0.8 30,038 + ABIE53_003891 0.13 +2.4 30,039 - ABIE53_003891 0.13 -1.2 30,039 - ABIE53_003891 0.13 +0.8 30,164 + ABIE53_003891 0.40 -1.2 30,165 - ABIE53_003891 0.40 +0.3 30,165 - ABIE53_003891 0.40 -0.2 30,269 - ABIE53_003891 0.63 -3.1 30,729 - -0.5 30,730 + -2.0 30,730 + +2.1 30,730 + +0.2 30,731 - -0.2 30,942 + -0.2 30,943 - -1.9 30,955 + +0.1 30,956 - +0.8 30,984 - +1.4 31,023 + +0.8 31,131 + ABIE53_003893 0.12 -0.2 31,132 - ABIE53_003893 0.13 -0.3 31,147 + ABIE53_003893 0.15 -1.5 31,148 - ABIE53_003893 0.15 -0.5 31,178 - ABIE53_003893 0.19 -1.8 31,178 - ABIE53_003893 0.19 -0.8 31,200 - ABIE53_003893 0.21 +0.8 31,208 - ABIE53_003893 0.22 +0.1 31,300 + ABIE53_003893 0.35 +2.4 31,342 + ABIE53_003893 0.40 -1.1 31,343 - ABIE53_003893 0.40 +1.1 31,343 - ABIE53_003893 0.40 -0.6 31,343 - ABIE53_003893 0.40 -0.1 31,343 - ABIE53_003893 0.40 -0.2 31,343 - ABIE53_003893 0.40 +2.1 31,343 - ABIE53_003893 0.40 +3.6 31,417 + ABIE53_003893 0.50 +1.0 31,417 + ABIE53_003893 0.50 -0.5
Or see this region's nucleotide sequence