Experiment: R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000794 and ABIE53_000795 are separated by 30 nucleotides ABIE53_000795 and ABIE53_000796 are separated by 9 nucleotides
ABIE53_000794: ABIE53_000794 - ABC-type transport system involved in Fe-S cluster assembly fused permease/ATPase subunit, at 859,689 to 861,599
_000794
ABIE53_000795: ABIE53_000795 - MurNAc alpha-1-phosphate uridylyltransferase, at 861,630 to 862,337
_000795
ABIE53_000796: ABIE53_000796 - aminoglycoside/choline kinase family phosphotransferase, at 862,347 to 863,459
_000796
Position (kb)
861
862
863 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 860.649 kb on + strand, within ABIE53_000794 at 860.649 kb on + strand, within ABIE53_000794 at 860.649 kb on + strand, within ABIE53_000794 at 860.649 kb on + strand, within ABIE53_000794 at 860.649 kb on + strand, within ABIE53_000794 at 860.650 kb on - strand, within ABIE53_000794 at 860.650 kb on - strand, within ABIE53_000794 at 860.650 kb on - strand, within ABIE53_000794 at 860.650 kb on - strand, within ABIE53_000794 at 860.650 kb on - strand, within ABIE53_000794 at 860.651 kb on + strand, within ABIE53_000794 at 860.651 kb on + strand, within ABIE53_000794 at 860.652 kb on - strand, within ABIE53_000794 at 860.652 kb on - strand, within ABIE53_000794 at 860.680 kb on - strand, within ABIE53_000794 at 860.823 kb on + strand, within ABIE53_000794 at 860.824 kb on - strand, within ABIE53_000794 at 860.900 kb on + strand, within ABIE53_000794 at 860.901 kb on - strand, within ABIE53_000794 at 860.901 kb on - strand, within ABIE53_000794 at 860.946 kb on + strand, within ABIE53_000794 at 860.946 kb on + strand, within ABIE53_000794 at 860.946 kb on + strand, within ABIE53_000794 at 860.946 kb on + strand, within ABIE53_000794 at 860.947 kb on - strand, within ABIE53_000794 at 860.947 kb on - strand, within ABIE53_000794 at 860.947 kb on - strand, within ABIE53_000794 at 860.947 kb on - strand, within ABIE53_000794 at 860.947 kb on - strand, within ABIE53_000794 at 861.078 kb on + strand, within ABIE53_000794 at 861.078 kb on + strand, within ABIE53_000794 at 861.079 kb on - strand, within ABIE53_000794 at 861.081 kb on + strand, within ABIE53_000794 at 861.081 kb on + strand, within ABIE53_000794 at 861.081 kb on + strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.082 kb on - strand, within ABIE53_000794 at 861.093 kb on + strand, within ABIE53_000794 at 861.093 kb on + strand, within ABIE53_000794 at 861.094 kb on - strand, within ABIE53_000794 at 861.149 kb on + strand, within ABIE53_000794 at 861.149 kb on + strand, within ABIE53_000794 at 861.150 kb on - strand, within ABIE53_000794 at 861.183 kb on + strand, within ABIE53_000794 at 861.183 kb on + strand, within ABIE53_000794 at 861.183 kb on + strand, within ABIE53_000794 at 861.183 kb on + strand, within ABIE53_000794 at 861.184 kb on - strand, within ABIE53_000794 at 861.280 kb on + strand, within ABIE53_000794 at 861.280 kb on + strand, within ABIE53_000794 at 861.280 kb on + strand, within ABIE53_000794 at 861.280 kb on + strand, within ABIE53_000794 at 861.281 kb on - strand, within ABIE53_000794 at 861.281 kb on - strand, within ABIE53_000794 at 861.281 kb on - strand, within ABIE53_000794 at 861.703 kb on + strand, within ABIE53_000795 at 861.703 kb on + strand, within ABIE53_000795 at 861.703 kb on + strand, within ABIE53_000795 at 861.703 kb on + strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.704 kb on - strand, within ABIE53_000795 at 861.706 kb on - strand, within ABIE53_000795 at 861.706 kb on - strand, within ABIE53_000795 at 861.748 kb on + strand, within ABIE53_000795 at 861.749 kb on - strand, within ABIE53_000795 at 861.749 kb on - strand, within ABIE53_000795 at 861.806 kb on - strand, within ABIE53_000795 at 861.806 kb on - strand, within ABIE53_000795 at 861.976 kb on + strand, within ABIE53_000795 at 861.976 kb on + strand, within ABIE53_000795 at 861.991 kb on + strand, within ABIE53_000795 at 861.992 kb on - strand, within ABIE53_000795 at 861.992 kb on - strand, within ABIE53_000795 at 862.049 kb on + strand, within ABIE53_000795 at 862.050 kb on - strand, within ABIE53_000795 at 862.102 kb on + strand, within ABIE53_000795 at 862.102 kb on + strand, within ABIE53_000795 at 862.103 kb on - strand, within ABIE53_000795 at 862.103 kb on - strand, within ABIE53_000795 at 862.357 kb on + strand at 862.358 kb on - strand at 862.423 kb on + strand at 862.423 kb on + strand at 862.424 kb on - strand at 862.424 kb on - strand at 862.424 kb on - strand at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.477 kb on + strand, within ABIE53_000796 at 862.478 kb on - strand, within ABIE53_000796 at 862.478 kb on - strand, within ABIE53_000796 at 862.478 kb on - strand, within ABIE53_000796 at 862.478 kb on - strand, within ABIE53_000796 at 862.478 kb on - strand, within ABIE53_000796 at 862.498 kb on + strand, within ABIE53_000796 at 862.532 kb on + strand, within ABIE53_000796 at 862.532 kb on + strand, within ABIE53_000796 at 862.532 kb on + strand, within ABIE53_000796 at 862.532 kb on + strand, within ABIE53_000796 at 862.532 kb on + strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.533 kb on - strand, within ABIE53_000796 at 862.567 kb on + strand, within ABIE53_000796 at 862.567 kb on + strand, within ABIE53_000796 at 862.568 kb on - strand, within ABIE53_000796 at 862.568 kb on - strand, within ABIE53_000796 at 862.624 kb on + strand, within ABIE53_000796 at 862.624 kb on + strand, within ABIE53_000796 at 862.627 kb on + strand, within ABIE53_000796 at 862.627 kb on + strand, within ABIE53_000796 at 862.627 kb on + strand, within ABIE53_000796 at 862.627 kb on + strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.628 kb on - strand, within ABIE53_000796 at 862.699 kb on + strand, within ABIE53_000796 at 862.699 kb on + strand, within ABIE53_000796 at 862.699 kb on + strand, within ABIE53_000796 at 862.699 kb on + strand, within ABIE53_000796 at 862.699 kb on + strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.700 kb on - strand, within ABIE53_000796 at 862.716 kb on + strand, within ABIE53_000796 at 862.716 kb on + strand, within ABIE53_000796 at 862.717 kb on - strand, within ABIE53_000796 at 862.895 kb on + strand, within ABIE53_000796 at 863.107 kb on + strand, within ABIE53_000796 at 863.108 kb on - strand, within ABIE53_000796 at 863.292 kb on - strand, within ABIE53_000796 at 863.308 kb on + strand, within ABIE53_000796 at 863.309 kb on - strand, within ABIE53_000796 at 863.309 kb on - strand, within ABIE53_000796
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM remove 860,649 + ABIE53_000794 0.50 +0.8 860,649 + ABIE53_000794 0.50 -0.5 860,649 + ABIE53_000794 0.50 -0.7 860,649 + ABIE53_000794 0.50 -0.4 860,649 + ABIE53_000794 0.50 -0.7 860,650 - ABIE53_000794 0.50 -0.6 860,650 - ABIE53_000794 0.50 -0.7 860,650 - ABIE53_000794 0.50 +2.4 860,650 - ABIE53_000794 0.50 -0.2 860,650 - ABIE53_000794 0.50 +0.1 860,651 + ABIE53_000794 0.50 +0.2 860,651 + ABIE53_000794 0.50 -0.2 860,652 - ABIE53_000794 0.50 +1.8 860,652 - ABIE53_000794 0.50 +2.4 860,680 - ABIE53_000794 0.52 +0.1 860,823 + ABIE53_000794 0.59 -0.2 860,824 - ABIE53_000794 0.59 +1.3 860,900 + ABIE53_000794 0.63 +1.4 860,901 - ABIE53_000794 0.63 -1.0 860,901 - ABIE53_000794 0.63 +0.1 860,946 + ABIE53_000794 0.66 -0.6 860,946 + ABIE53_000794 0.66 +2.8 860,946 + ABIE53_000794 0.66 +1.0 860,946 + ABIE53_000794 0.66 -0.2 860,947 - ABIE53_000794 0.66 +1.8 860,947 - ABIE53_000794 0.66 +0.8 860,947 - ABIE53_000794 0.66 +1.8 860,947 - ABIE53_000794 0.66 +1.0 860,947 - ABIE53_000794 0.66 -0.5 861,078 + ABIE53_000794 0.73 -1.7 861,078 + ABIE53_000794 0.73 +2.4 861,079 - ABIE53_000794 0.73 -0.6 861,081 + ABIE53_000794 0.73 -0.5 861,081 + ABIE53_000794 0.73 -0.2 861,081 + ABIE53_000794 0.73 +0.4 861,082 - ABIE53_000794 0.73 +1.8 861,082 - ABIE53_000794 0.73 +0.8 861,082 - ABIE53_000794 0.73 +2.4 861,082 - ABIE53_000794 0.73 +1.4 861,082 - ABIE53_000794 0.73 -1.2 861,082 - ABIE53_000794 0.73 +1.8 861,093 + ABIE53_000794 0.73 +0.4 861,093 + ABIE53_000794 0.73 +0.8 861,094 - ABIE53_000794 0.74 -1.3 861,149 + ABIE53_000794 0.76 +3.0 861,149 + ABIE53_000794 0.76 -0.5 861,150 - ABIE53_000794 0.76 +0.1 861,183 + ABIE53_000794 0.78 +0.4 861,183 + ABIE53_000794 0.78 -0.2 861,183 + ABIE53_000794 0.78 -0.6 861,183 + ABIE53_000794 0.78 -1.2 861,184 - ABIE53_000794 0.78 -0.1 861,280 + ABIE53_000794 0.83 -1.2 861,280 + ABIE53_000794 0.83 -2.2 861,280 + ABIE53_000794 0.83 -0.2 861,280 + ABIE53_000794 0.83 +0.8 861,281 - ABIE53_000794 0.83 -0.3 861,281 - ABIE53_000794 0.83 +0.8 861,281 - ABIE53_000794 0.83 -1.7 861,703 + ABIE53_000795 0.10 -2.3 861,703 + ABIE53_000795 0.10 -0.2 861,703 + ABIE53_000795 0.10 -1.2 861,703 + ABIE53_000795 0.10 +1.8 861,704 - ABIE53_000795 0.10 +2.4 861,704 - ABIE53_000795 0.10 -0.4 861,704 - ABIE53_000795 0.10 -2.0 861,704 - ABIE53_000795 0.10 -2.2 861,704 - ABIE53_000795 0.10 +0.8 861,704 - ABIE53_000795 0.10 +1.8 861,704 - ABIE53_000795 0.10 +2.4 861,706 - ABIE53_000795 0.11 -0.2 861,706 - ABIE53_000795 0.11 -0.2 861,748 + ABIE53_000795 0.17 -0.7 861,749 - ABIE53_000795 0.17 -1.2 861,749 - ABIE53_000795 0.17 -0.7 861,806 - ABIE53_000795 0.25 -2.6 861,806 - ABIE53_000795 0.25 -1.3 861,976 + ABIE53_000795 0.49 +0.8 861,976 + ABIE53_000795 0.49 -0.2 861,991 + ABIE53_000795 0.51 -2.6 861,992 - ABIE53_000795 0.51 -0.2 861,992 - ABIE53_000795 0.51 -2.6 862,049 + ABIE53_000795 0.59 -0.7 862,050 - ABIE53_000795 0.59 -0.2 862,102 + ABIE53_000795 0.67 -1.2 862,102 + ABIE53_000795 0.67 -0.7 862,103 - ABIE53_000795 0.67 -0.7 862,103 - ABIE53_000795 0.67 -2.6 862,357 + -0.2 862,358 - +1.2 862,423 + +0.6 862,423 + +1.8 862,424 - +0.8 862,424 - -0.2 862,424 - +0.8 862,477 + ABIE53_000796 0.12 +0.8 862,477 + ABIE53_000796 0.12 +2.4 862,477 + ABIE53_000796 0.12 -1.7 862,477 + ABIE53_000796 0.12 +0.2 862,477 + ABIE53_000796 0.12 -0.6 862,477 + ABIE53_000796 0.12 +0.8 862,477 + ABIE53_000796 0.12 +1.3 862,477 + ABIE53_000796 0.12 +1.2 862,477 + ABIE53_000796 0.12 -0.3 862,478 - ABIE53_000796 0.12 +0.3 862,478 - ABIE53_000796 0.12 -1.2 862,478 - ABIE53_000796 0.12 +0.1 862,478 - ABIE53_000796 0.12 -0.4 862,478 - ABIE53_000796 0.12 +0.1 862,498 + ABIE53_000796 0.14 -1.0 862,532 + ABIE53_000796 0.17 +0.0 862,532 + ABIE53_000796 0.17 -0.3 862,532 + ABIE53_000796 0.17 -1.5 862,532 + ABIE53_000796 0.17 +0.8 862,532 + ABIE53_000796 0.17 +0.0 862,533 - ABIE53_000796 0.17 +1.4 862,533 - ABIE53_000796 0.17 -0.5 862,533 - ABIE53_000796 0.17 -2.0 862,533 - ABIE53_000796 0.17 +0.1 862,533 - ABIE53_000796 0.17 -0.6 862,533 - ABIE53_000796 0.17 -1.2 862,533 - ABIE53_000796 0.17 -1.6 862,533 - ABIE53_000796 0.17 +1.0 862,533 - ABIE53_000796 0.17 +1.4 862,533 - ABIE53_000796 0.17 +0.8 862,533 - ABIE53_000796 0.17 +1.8 862,533 - ABIE53_000796 0.17 -1.3 862,567 + ABIE53_000796 0.20 +1.3 862,567 + ABIE53_000796 0.20 -1.6 862,568 - ABIE53_000796 0.20 +0.8 862,568 - ABIE53_000796 0.20 -0.2 862,624 + ABIE53_000796 0.25 -0.2 862,624 + ABIE53_000796 0.25 -1.3 862,627 + ABIE53_000796 0.25 +0.6 862,627 + ABIE53_000796 0.25 -0.4 862,627 + ABIE53_000796 0.25 +0.3 862,627 + ABIE53_000796 0.25 -0.5 862,628 - ABIE53_000796 0.25 -1.9 862,628 - ABIE53_000796 0.25 +1.4 862,628 - ABIE53_000796 0.25 -2.5 862,628 - ABIE53_000796 0.25 -0.7 862,628 - ABIE53_000796 0.25 +0.4 862,628 - ABIE53_000796 0.25 +0.8 862,628 - ABIE53_000796 0.25 -0.2 862,699 + ABIE53_000796 0.32 -0.7 862,699 + ABIE53_000796 0.32 -0.2 862,699 + ABIE53_000796 0.32 -0.3 862,699 + ABIE53_000796 0.32 -0.6 862,699 + ABIE53_000796 0.32 +0.1 862,700 - ABIE53_000796 0.32 -0.5 862,700 - ABIE53_000796 0.32 -0.0 862,700 - ABIE53_000796 0.32 +1.0 862,700 - ABIE53_000796 0.32 +0.8 862,700 - ABIE53_000796 0.32 +1.8 862,700 - ABIE53_000796 0.32 +1.5 862,716 + ABIE53_000796 0.33 +2.4 862,716 + ABIE53_000796 0.33 +0.8 862,717 - ABIE53_000796 0.33 +0.5 862,895 + ABIE53_000796 0.49 -0.7 863,107 + ABIE53_000796 0.68 +0.9 863,108 - ABIE53_000796 0.68 -0.5 863,292 - ABIE53_000796 0.85 -0.3 863,308 + ABIE53_000796 0.86 +0.8 863,309 - ABIE53_000796 0.86 -0.5 863,309 - ABIE53_000796 0.86 -0.3
Or see this region's nucleotide sequence