Experiment: MoLS4 with L-Leucine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purD and paaK-1 are separated by 167 nucleotides paaK-1 and DORF5594 are separated by 689 nucleotides
DVU0488: purD - phosphoribosylamine--glycine ligase (TIGR), at 555,143 to 556,417
purD
DVU0489: paaK-1 - phenylacetate-coenzyme A ligase (TIGR), at 556,585 to 557,883
paaK-1
DORF5594: DORF5594 - SEED: Predicted signal transduction protein, at 558,573 to 559,487
DORF5594
Position (kb) 556
557
558 Strain fitness (log2 ratio) -2
-1
0
1
2 at 555.808 kb on - strand, within purD at 555.860 kb on - strand, within purD at 555.890 kb on + strand, within purD at 555.958 kb on - strand, within purD at 556.180 kb on + strand, within purD at 556.204 kb on + strand, within purD at 556.336 kb on + strand at 556.431 kb on - strand at 556.498 kb on + strand at 556.575 kb on + strand at 556.629 kb on + strand at 556.700 kb on + strand at 556.776 kb on - strand, within paaK-1 at 556.823 kb on - strand, within paaK-1 at 556.845 kb on + strand, within paaK-1 at 556.875 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.889 kb on + strand, within paaK-1 at 556.890 kb on - strand, within paaK-1 at 556.895 kb on - strand, within paaK-1 at 556.895 kb on - strand, within paaK-1 at 556.897 kb on - strand, within paaK-1 at 556.921 kb on - strand, within paaK-1 at 556.968 kb on - strand, within paaK-1 at 556.972 kb on + strand, within paaK-1 at 556.988 kb on + strand, within paaK-1 at 557.005 kb on + strand, within paaK-1 at 557.015 kb on + strand, within paaK-1 at 557.145 kb on + strand, within paaK-1 at 557.145 kb on + strand, within paaK-1 at 557.194 kb on - strand, within paaK-1 at 557.194 kb on - strand, within paaK-1 at 557.196 kb on + strand, within paaK-1 at 557.254 kb on - strand, within paaK-1 at 557.266 kb on + strand, within paaK-1 at 557.321 kb on + strand, within paaK-1 at 557.400 kb on + strand, within paaK-1 at 557.446 kb on - strand, within paaK-1 at 557.595 kb on + strand, within paaK-1 at 557.595 kb on + strand, within paaK-1 at 557.603 kb on - strand, within paaK-1 at 557.688 kb on + strand, within paaK-1 at 557.697 kb on + strand, within paaK-1 at 557.760 kb on + strand at 557.782 kb on + strand at 557.829 kb on - strand at 557.829 kb on - strand at 557.844 kb on + strand at 557.844 kb on + strand at 557.871 kb on + strand at 557.878 kb on + strand at 557.886 kb on - strand at 557.886 kb on - strand at 557.888 kb on - strand at 557.888 kb on - strand at 557.934 kb on - strand at 557.936 kb on - strand at 557.967 kb on + strand at 557.988 kb on - strand at 558.112 kb on - strand at 558.127 kb on - strand at 558.252 kb on + strand at 558.260 kb on - strand at 558.315 kb on + strand at 558.411 kb on + strand at 558.419 kb on - strand at 558.455 kb on + strand at 558.463 kb on - strand at 558.555 kb on + strand at 558.571 kb on + strand at 558.603 kb on + strand at 558.611 kb on - strand at 558.723 kb on + strand, within DORF5594 at 558.740 kb on - strand, within DORF5594 at 558.763 kb on + strand, within DORF5594 at 558.765 kb on + strand, within DORF5594 at 558.810 kb on - strand, within DORF5594
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Leucine as nutrient 10mM remove 555,808 - purD DVU0488 0.52 -0.5 555,860 - purD DVU0488 0.56 -0.9 555,890 + purD DVU0488 0.59 -2.0 555,958 - purD DVU0488 0.64 -1.2 556,180 + purD DVU0488 0.81 -0.5 556,204 + purD DVU0488 0.83 -2.7 556,336 + -0.5 556,431 - -0.7 556,498 + +0.4 556,575 + +0.7 556,629 + -0.6 556,700 + -1.4 556,776 - paaK-1 DVU0489 0.15 +0.2 556,823 - paaK-1 DVU0489 0.18 -0.1 556,845 + paaK-1 DVU0489 0.20 +0.3 556,875 + paaK-1 DVU0489 0.22 -1.0 556,887 + paaK-1 DVU0489 0.23 -0.0 556,887 + paaK-1 DVU0489 0.23 +0.3 556,887 + paaK-1 DVU0489 0.23 +0.2 556,889 + paaK-1 DVU0489 0.23 +0.2 556,890 - paaK-1 DVU0489 0.23 +0.4 556,895 - paaK-1 DVU0489 0.24 -0.0 556,895 - paaK-1 DVU0489 0.24 +0.9 556,897 - paaK-1 DVU0489 0.24 -1.3 556,921 - paaK-1 DVU0489 0.26 +0.6 556,968 - paaK-1 DVU0489 0.29 +0.9 556,972 + paaK-1 DVU0489 0.30 +1.0 556,988 + paaK-1 DVU0489 0.31 +2.3 557,005 + paaK-1 DVU0489 0.32 +0.0 557,015 + paaK-1 DVU0489 0.33 +0.5 557,145 + paaK-1 DVU0489 0.43 -0.5 557,145 + paaK-1 DVU0489 0.43 -1.5 557,194 - paaK-1 DVU0489 0.47 +0.8 557,194 - paaK-1 DVU0489 0.47 +0.3 557,196 + paaK-1 DVU0489 0.47 -1.5 557,254 - paaK-1 DVU0489 0.52 -0.8 557,266 + paaK-1 DVU0489 0.52 +0.4 557,321 + paaK-1 DVU0489 0.57 +1.1 557,400 + paaK-1 DVU0489 0.63 +0.5 557,446 - paaK-1 DVU0489 0.66 -0.6 557,595 + paaK-1 DVU0489 0.78 -0.4 557,595 + paaK-1 DVU0489 0.78 +0.3 557,603 - paaK-1 DVU0489 0.78 -0.4 557,688 + paaK-1 DVU0489 0.85 +0.3 557,697 + paaK-1 DVU0489 0.86 +0.4 557,760 + +1.1 557,782 + -1.4 557,829 - -1.1 557,829 - -0.7 557,844 + -0.4 557,844 + -0.8 557,871 + -0.0 557,878 + +1.0 557,886 - -1.5 557,886 - -1.2 557,888 - -0.0 557,888 - +0.4 557,934 - -0.3 557,936 - +0.5 557,967 + -0.3 557,988 - -1.9 558,112 - -0.1 558,127 - -1.2 558,252 + -0.8 558,260 - -0.6 558,315 + +0.3 558,411 + -0.9 558,419 - -0.3 558,455 + -0.6 558,463 - -0.7 558,555 + +0.3 558,571 + +0.5 558,603 + -0.5 558,611 - -0.0 558,723 + DORF5594 0.16 +1.0 558,740 - DORF5594 0.18 +0.2 558,763 + DORF5594 0.21 +0.8 558,765 + DORF5594 0.21 +0.2 558,810 - DORF5594 0.26 +0.3
Or see this region's nucleotide sequence