Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS00645 and AZOBR_RS00650 overlap by 4 nucleotides AZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotides AZOBR_RS00655 and AZOBR_RS00660 overlap by 1 nucleotides AZOBR_RS00660 and AZOBR_RS00665 are separated by 206 nucleotides
AZOBR_RS00645: AZOBR_RS00645 - magnesium transporter, at 129,587 to 130,576
_RS00645
AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481
_RS00650
AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833
_RS00655
AZOBR_RS00660: AZOBR_RS00660 - hypothetical protein, at 131,833 to 132,117
_RS00660
AZOBR_RS00665: AZOBR_RS00665 - tRNA-Ala, at 132,324 to 132,399
_RS00665
Position (kb) 130
131
132 Strain fitness (log2 ratio) -2
-1
0
1
2 at 129.588 kb on + strand at 129.588 kb on + strand at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.356 kb on + strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.357 kb on - strand, within AZOBR_RS00645 at 130.378 kb on - strand, within AZOBR_RS00645 at 131.849 kb on + strand at 132.075 kb on - strand, within AZOBR_RS00660 at 132.366 kb on + strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665 at 132.367 kb on - strand, within AZOBR_RS00665
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 129,588 + +2.6 129,588 + -1.5 130,356 + AZOBR_RS00645 0.78 +1.6 130,356 + AZOBR_RS00645 0.78 +0.6 130,356 + AZOBR_RS00645 0.78 -1.6 130,357 - AZOBR_RS00645 0.78 -2.3 130,357 - AZOBR_RS00645 0.78 -1.5 130,378 - AZOBR_RS00645 0.80 +0.6 131,849 + +1.3 132,075 - AZOBR_RS00660 0.85 +0.0 132,366 + AZOBR_RS00665 0.55 -1.0 132,367 - AZOBR_RS00665 0.57 -0.2 132,367 - AZOBR_RS00665 0.57 +1.3
Or see this region's nucleotide sequence