Experiment: R2A with N-(3-oxohexanoyl)-DL-homoserine lactone 10 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_005273 and ABIE53_005274 overlap by 4 nucleotides ABIE53_005274 and ABIE53_005275 are separated by 279 nucleotides ABIE53_005275 and ABIE53_005276 are separated by 45 nucleotides
ABIE53_005273: ABIE53_005273 - NAD+ synthase (glutamine-hydrolyzing), at 1,535,618 to 1,537,666
_005273
ABIE53_005274: ABIE53_005274 - nicotinamide-nucleotide amidase, at 1,537,663 to 1,538,178
_005274
ABIE53_005275: ABIE53_005275 - GNAT superfamily N-acetyltransferase, at 1,538,458 to 1,538,967
_005275
ABIE53_005276: ABIE53_005276 - mono/diheme cytochrome c family protein, at 1,539,013 to 1,540,335
_005276
Position (kb) 1538
1539 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1537.704 kb on - strand at 1537.728 kb on - strand, within ABIE53_005274 at 1537.755 kb on + strand, within ABIE53_005274 at 1537.793 kb on + strand, within ABIE53_005274 at 1537.793 kb on + strand, within ABIE53_005274 at 1537.793 kb on + strand, within ABIE53_005274 at 1537.794 kb on - strand, within ABIE53_005274 at 1537.794 kb on - strand, within ABIE53_005274 at 1537.900 kb on - strand, within ABIE53_005274 at 1538.172 kb on + strand at 1538.310 kb on - strand at 1538.682 kb on + strand, within ABIE53_005275 at 1538.875 kb on + strand, within ABIE53_005275 at 1538.875 kb on + strand, within ABIE53_005275 at 1539.014 kb on + strand at 1539.019 kb on - strand at 1539.019 kb on - strand at 1539.323 kb on + strand, within ABIE53_005276 at 1539.404 kb on + strand, within ABIE53_005276 at 1539.444 kb on - strand, within ABIE53_005276 at 1539.480 kb on - strand, within ABIE53_005276 at 1539.653 kb on + strand, within ABIE53_005276 at 1539.653 kb on + strand, within ABIE53_005276 at 1539.658 kb on - strand, within ABIE53_005276 at 1539.658 kb on - strand, within ABIE53_005276 at 1539.828 kb on - strand, within ABIE53_005276 at 1539.859 kb on + strand, within ABIE53_005276 at 1539.859 kb on + strand, within ABIE53_005276 at 1539.860 kb on - strand, within ABIE53_005276 at 1539.860 kb on - strand, within ABIE53_005276 at 1539.912 kb on - strand, within ABIE53_005276 at 1539.963 kb on - strand, within ABIE53_005276
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with N-(3-oxohexanoyl)-DL-homoserine lactone 10 uM remove 1,537,704 - -0.2 1,537,728 - ABIE53_005274 0.13 -1.1 1,537,755 + ABIE53_005274 0.18 -2.0 1,537,793 + ABIE53_005274 0.25 +2.0 1,537,793 + ABIE53_005274 0.25 -0.3 1,537,793 + ABIE53_005274 0.25 -0.5 1,537,794 - ABIE53_005274 0.25 -1.3 1,537,794 - ABIE53_005274 0.25 +0.5 1,537,900 - ABIE53_005274 0.46 -1.9 1,538,172 + -0.6 1,538,310 - +0.6 1,538,682 + ABIE53_005275 0.44 +0.1 1,538,875 + ABIE53_005275 0.82 +1.0 1,538,875 + ABIE53_005275 0.82 -1.9 1,539,014 + -1.6 1,539,019 - -0.0 1,539,019 - -0.4 1,539,323 + ABIE53_005276 0.23 +1.0 1,539,404 + ABIE53_005276 0.30 -1.2 1,539,444 - ABIE53_005276 0.33 +1.0 1,539,480 - ABIE53_005276 0.35 -0.0 1,539,653 + ABIE53_005276 0.48 -1.6 1,539,653 + ABIE53_005276 0.48 -0.0 1,539,658 - ABIE53_005276 0.49 +1.0 1,539,658 - ABIE53_005276 0.49 +1.6 1,539,828 - ABIE53_005276 0.62 +0.1 1,539,859 + ABIE53_005276 0.64 -1.2 1,539,859 + ABIE53_005276 0.64 +1.7 1,539,860 - ABIE53_005276 0.64 -1.0 1,539,860 - ABIE53_005276 0.64 -2.1 1,539,912 - ABIE53_005276 0.68 +1.7 1,539,963 - ABIE53_005276 0.72 +1.0
Or see this region's nucleotide sequence