Experiment: MoLS4 with L-Leucine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadB and DVU1810 are separated by 110 nucleotides DVU1810 and DVU1811 are separated by 17 nucleotides DVU1811 and coxB are separated by 43 nucleotides
DVU1809: nadB - L-aspartate oxidase (TIGR), at 1,873,643 to 1,875,229
nadB
DVU1810: DVU1810 - hypothetical protein (TIGR), at 1,875,340 to 1,875,762
DVU1810
DVU1811: DVU1811 - protoheme IX farnesyltransferase, putative (TIGR), at 1,875,780 to 1,876,643
DVU1811
DVU1812: coxB - cytochrome c oxidase, subunit II, putative (TIGR), at 1,876,687 to 1,877,967
coxB
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1875.300 kb on + strand at 1875.404 kb on + strand, within DVU1810 at 1875.608 kb on - strand, within DVU1810 at 1875.711 kb on + strand, within DVU1810 at 1875.720 kb on + strand at 1875.724 kb on - strand at 1875.786 kb on - strand at 1875.921 kb on - strand, within DVU1811 at 1875.931 kb on - strand, within DVU1811 at 1876.042 kb on + strand, within DVU1811 at 1876.147 kb on - strand, within DVU1811 at 1876.149 kb on - strand, within DVU1811 at 1876.149 kb on - strand, within DVU1811 at 1876.278 kb on + strand, within DVU1811 at 1876.343 kb on - strand, within DVU1811 at 1876.371 kb on + strand, within DVU1811 at 1876.418 kb on - strand, within DVU1811 at 1876.419 kb on + strand, within DVU1811 at 1876.427 kb on - strand, within DVU1811 at 1876.491 kb on + strand, within DVU1811 at 1876.628 kb on - strand at 1876.630 kb on + strand at 1876.858 kb on - strand, within coxB at 1876.891 kb on + strand, within coxB at 1876.926 kb on - strand, within coxB at 1876.971 kb on - strand, within coxB at 1877.015 kb on + strand, within coxB at 1877.028 kb on - strand, within coxB at 1877.114 kb on - strand, within coxB at 1877.165 kb on + strand, within coxB at 1877.165 kb on + strand, within coxB at 1877.188 kb on + strand, within coxB at 1877.260 kb on + strand, within coxB at 1877.263 kb on - strand, within coxB at 1877.268 kb on - strand, within coxB at 1877.348 kb on + strand, within coxB at 1877.489 kb on + strand, within coxB at 1877.499 kb on + strand, within coxB at 1877.514 kb on - strand, within coxB at 1877.549 kb on + strand, within coxB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Leucine as nutrient 10mM remove 1,875,300 + -0.5 1,875,404 + DVU1810 0.15 -1.9 1,875,608 - DVU1810 0.63 +0.3 1,875,711 + DVU1810 0.88 +1.4 1,875,720 + -1.3 1,875,724 - +0.2 1,875,786 - -1.6 1,875,921 - DVU1811 0.16 -0.9 1,875,931 - DVU1811 0.17 -1.4 1,876,042 + DVU1811 0.30 -0.0 1,876,147 - DVU1811 0.42 -0.4 1,876,149 - DVU1811 0.43 +0.2 1,876,149 - DVU1811 0.43 -0.1 1,876,278 + DVU1811 0.58 +2.4 1,876,343 - DVU1811 0.65 +1.1 1,876,371 + DVU1811 0.68 +0.5 1,876,418 - DVU1811 0.74 +0.5 1,876,419 + DVU1811 0.74 +0.3 1,876,427 - DVU1811 0.75 -0.1 1,876,491 + DVU1811 0.82 -0.4 1,876,628 - -0.4 1,876,630 + +0.2 1,876,858 - coxB DVU1812 0.13 -1.4 1,876,891 + coxB DVU1812 0.16 -0.3 1,876,926 - coxB DVU1812 0.19 -0.4 1,876,971 - coxB DVU1812 0.22 +0.8 1,877,015 + coxB DVU1812 0.26 +0.2 1,877,028 - coxB DVU1812 0.27 -0.3 1,877,114 - coxB DVU1812 0.33 +0.1 1,877,165 + coxB DVU1812 0.37 -0.1 1,877,165 + coxB DVU1812 0.37 -0.5 1,877,188 + coxB DVU1812 0.39 -0.1 1,877,260 + coxB DVU1812 0.45 -0.0 1,877,263 - coxB DVU1812 0.45 -0.4 1,877,268 - coxB DVU1812 0.45 +1.5 1,877,348 + coxB DVU1812 0.52 -1.8 1,877,489 + coxB DVU1812 0.63 +0.8 1,877,499 + coxB DVU1812 0.63 +0.1 1,877,514 - coxB DVU1812 0.65 -0.1 1,877,549 + coxB DVU1812 0.67 -0.8
Or see this region's nucleotide sequence