Experiment: KB with Gossypetin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2222 and Psyr_2223 overlap by 4 nucleotides Psyr_2223 and Psyr_2224 are separated by 13 nucleotides Psyr_2224 and Psyr_2225 overlap by 19 nucleotides Psyr_2225 and Psyr_2226 overlap by 4 nucleotides
Psyr_2222: Psyr_2222 - aminomethyltransferase, at 2,582,408 to 2,585,314
_2222
Psyr_2223: Psyr_2223 - sarcosine oxidase, delta subunit, at 2,585,311 to 2,585,616
_2223
Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871
_2224
Psyr_2225: Psyr_2225 - methenyltetrahydrofolate cyclohydrolase / 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 2,586,853 to 2,587,755
_2225
Psyr_2226: Psyr_2226 - formyltetrahydrofolate deformylase, at 2,587,752 to 2,588,618
_2226
Position (kb)
2585
2586
2587 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2584.696 kb on - strand, within Psyr_2222 at 2584.696 kb on - strand, within Psyr_2222 at 2584.824 kb on + strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.840 kb on - strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.930 kb on - strand, within Psyr_2222 at 2584.945 kb on - strand, within Psyr_2222 at 2585.069 kb on + strand at 2585.070 kb on - strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.226 kb on + strand at 2585.226 kb on + strand at 2585.498 kb on + strand, within Psyr_2223 at 2585.499 kb on - strand, within Psyr_2223 at 2585.808 kb on + strand, within Psyr_2224 at 2585.808 kb on + strand, within Psyr_2224 at 2585.811 kb on + strand, within Psyr_2224 at 2585.812 kb on - strand at 2585.812 kb on - strand, within Psyr_2224 at 2585.847 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.543 kb on + strand, within Psyr_2224 at 2586.544 kb on - strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.603 kb on + strand, within Psyr_2224 at 2586.604 kb on - strand, within Psyr_2224 at 2586.604 kb on - strand, within Psyr_2224 at 2586.631 kb on - strand, within Psyr_2224 at 2586.706 kb on + strand, within Psyr_2224 at 2586.707 kb on - strand, within Psyr_2224 at 2586.720 kb on + strand, within Psyr_2224 at 2586.720 kb on + strand, within Psyr_2224 at 2586.721 kb on - strand, within Psyr_2224 at 2586.732 kb on + strand, within Psyr_2224 at 2586.733 kb on - strand, within Psyr_2224 at 2586.733 kb on - strand, within Psyr_2224 at 2586.736 kb on - strand, within Psyr_2224 at 2586.857 kb on + strand at 2586.948 kb on + strand, within Psyr_2225 at 2587.349 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.607 kb on - strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.610 kb on + strand, within Psyr_2225 at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.807 kb on + strand at 2587.808 kb on - strand at 2587.808 kb on - strand at 2587.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Gossypetin 10 mM remove 2,584,696 - Psyr_2222 0.79 +0.1 2,584,696 - Psyr_2222 0.79 -0.6 2,584,824 + Psyr_2222 0.83 +0.3 2,584,825 - Psyr_2222 0.83 -0.5 2,584,825 - Psyr_2222 0.83 -0.3 2,584,825 - Psyr_2222 0.83 +0.0 2,584,825 - Psyr_2222 0.83 -1.4 2,584,840 - Psyr_2222 0.84 +1.2 2,584,929 + Psyr_2222 0.87 -0.5 2,584,929 + Psyr_2222 0.87 +2.3 2,584,930 - Psyr_2222 0.87 -0.4 2,584,945 - Psyr_2222 0.87 -2.9 2,585,069 + -0.4 2,585,070 - -0.1 2,585,148 + +0.4 2,585,148 + +0.9 2,585,148 + -1.0 2,585,148 + +0.2 2,585,226 + +0.8 2,585,226 + -0.9 2,585,498 + Psyr_2223 0.61 -0.0 2,585,499 - Psyr_2223 0.61 -0.2 2,585,808 + Psyr_2224 0.14 -0.4 2,585,808 + Psyr_2224 0.14 +1.8 2,585,811 + Psyr_2224 0.15 +0.6 2,585,812 - +1.0 2,585,812 - Psyr_2224 0.15 -0.7 2,585,847 + Psyr_2224 0.17 +0.8 2,586,036 + Psyr_2224 0.33 +0.3 2,586,036 + Psyr_2224 0.33 +1.2 2,586,036 + Psyr_2224 0.33 +0.5 2,586,037 - Psyr_2224 0.33 +0.1 2,586,037 - Psyr_2224 0.33 +3.7 2,586,037 - Psyr_2224 0.33 -0.4 2,586,037 - Psyr_2224 0.33 +1.3 2,586,252 - Psyr_2224 0.50 +0.4 2,586,252 - Psyr_2224 0.50 +0.9 2,586,252 - Psyr_2224 0.50 +0.7 2,586,252 - Psyr_2224 0.50 -0.9 2,586,252 - Psyr_2224 0.50 +1.0 2,586,543 + Psyr_2224 0.74 +0.6 2,586,543 + Psyr_2224 0.74 -1.1 2,586,543 + Psyr_2224 0.74 +1.4 2,586,543 + Psyr_2224 0.74 -0.3 2,586,543 + Psyr_2224 0.74 +1.4 2,586,543 + Psyr_2224 0.74 -0.6 2,586,543 + Psyr_2224 0.74 -1.2 2,586,543 + Psyr_2224 0.74 +0.9 2,586,544 - Psyr_2224 0.74 -0.2 2,586,603 + Psyr_2224 0.78 +0.7 2,586,603 + Psyr_2224 0.78 +3.0 2,586,603 + Psyr_2224 0.78 +3.3 2,586,603 + Psyr_2224 0.78 +0.3 2,586,603 + Psyr_2224 0.78 +1.8 2,586,604 - Psyr_2224 0.78 +0.5 2,586,604 - Psyr_2224 0.78 -0.3 2,586,631 - Psyr_2224 0.81 -2.3 2,586,706 + Psyr_2224 0.87 +0.1 2,586,707 - Psyr_2224 0.87 -0.3 2,586,720 + Psyr_2224 0.88 -0.3 2,586,720 + Psyr_2224 0.88 +0.0 2,586,721 - Psyr_2224 0.88 -1.0 2,586,732 + Psyr_2224 0.89 -0.3 2,586,733 - Psyr_2224 0.89 -0.4 2,586,733 - Psyr_2224 0.89 +0.7 2,586,736 - Psyr_2224 0.89 -0.2 2,586,857 + +1.4 2,586,948 + Psyr_2225 0.11 +0.7 2,587,349 - Psyr_2225 0.55 +0.1 2,587,607 - Psyr_2225 0.83 +1.0 2,587,607 - Psyr_2225 0.83 -0.8 2,587,610 + Psyr_2225 0.84 -0.6 2,587,610 + Psyr_2225 0.84 +0.6 2,587,610 + Psyr_2225 0.84 +1.1 2,587,807 + -1.1 2,587,807 + +1.4 2,587,807 + -0.2 2,587,808 - -1.2 2,587,808 - +0.7 2,587,830 + +1.6
Or see this region's nucleotide sequence