Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1485 and HP15_1486 are separated by 21 nucleotides HP15_1486 and HP15_1487 overlap by 21 nucleotides HP15_1487 and HP15_1488 are separated by 22 nucleotides HP15_1488 and HP15_1489 are separated by 68 nucleotides HP15_1489 and HP15_1490 are separated by 63 nucleotides
HP15_1485: HP15_1485 - serine O-acetyltransferase, at 1,561,559 to 1,562,332
_1485
HP15_1486: HP15_1486 - hypothetical protein, at 1,562,354 to 1,562,470
_1486
HP15_1487: HP15_1487 - transcriptional regulator, BadM/Rrf2 family, at 1,562,450 to 1,562,908
_1487
HP15_1488: HP15_1488 - cysteine desulfurase IscS, at 1,562,931 to 1,564,079
_1488
HP15_1489: HP15_1489 - nucleoside diphosphate kinase, at 1,564,148 to 1,564,576
_1489
HP15_1490: HP15_1490 - conserved hypothetical protein, at 1,564,640 to 1,565,752
_1490
Position (kb)
1562
1563
1564
1565 Strain fitness (log2 ratio)
-1
0
1 at 1562.313 kb on + strand at 1562.727 kb on + strand, within HP15_1487 at 1562.734 kb on + strand, within HP15_1487 at 1562.739 kb on + strand, within HP15_1487 at 1564.584 kb on + strand at 1565.031 kb on + strand, within HP15_1490
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 1,562,313 + -0.9 1,562,727 + HP15_1487 0.60 +0.7 1,562,734 + HP15_1487 0.62 -1.2 1,562,739 + HP15_1487 0.63 -1.4 1,564,584 + +0.5 1,565,031 + HP15_1490 0.35 -0.1
Or see this region's nucleotide sequence