Experiment: BHIS with Sulfamonomethoxine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3127 and BT3128 are separated by 48 nucleotides BT3128 and BT3129 are separated by 161 nucleotides
BT3127: BT3127 - conserved hypothetical protein (NCBI ptt file), at 3,986,902 to 3,987,819
BT3127
BT3128: BT3128 - putative xanthosine triphosphate pyrophosphatase (NCBI ptt file), at 3,987,868 to 3,988,449
BT3128
BT3129: BT3129 - putative flagellar motor protein MotB (NCBI ptt file), at 3,988,611 to 3,989,453
BT3129
Position (kb)
3987
3988
3989 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3986.868 kb on - strand at 3986.878 kb on + strand at 3986.885 kb on + strand at 3986.886 kb on - strand at 3986.895 kb on + strand at 3986.895 kb on + strand at 3986.949 kb on + strand at 3986.953 kb on + strand at 3986.977 kb on + strand at 3987.031 kb on - strand, within BT3127 at 3987.088 kb on + strand, within BT3127 at 3987.132 kb on - strand, within BT3127 at 3987.134 kb on + strand, within BT3127 at 3987.134 kb on + strand, within BT3127 at 3987.135 kb on - strand, within BT3127 at 3987.163 kb on + strand, within BT3127 at 3987.164 kb on - strand, within BT3127 at 3987.212 kb on + strand, within BT3127 at 3987.220 kb on + strand, within BT3127 at 3987.224 kb on + strand, within BT3127 at 3987.225 kb on - strand, within BT3127 at 3987.295 kb on - strand, within BT3127 at 3987.344 kb on - strand, within BT3127 at 3987.560 kb on + strand, within BT3127 at 3987.619 kb on - strand, within BT3127 at 3987.717 kb on + strand, within BT3127 at 3987.739 kb on - strand at 3987.785 kb on - strand at 3987.818 kb on - strand at 3987.861 kb on + strand at 3987.862 kb on - strand at 3987.862 kb on - strand at 3987.884 kb on + strand at 3987.944 kb on + strand, within BT3128 at 3987.945 kb on - strand, within BT3128 at 3987.945 kb on - strand, within BT3128 at 3988.042 kb on + strand, within BT3128 at 3988.051 kb on + strand, within BT3128 at 3988.052 kb on - strand, within BT3128 at 3988.168 kb on - strand, within BT3128 at 3988.325 kb on - strand, within BT3128 at 3988.460 kb on + strand at 3988.552 kb on - strand at 3988.627 kb on + strand at 3988.640 kb on + strand at 3988.641 kb on - strand at 3988.641 kb on - strand at 3988.651 kb on + strand at 3988.651 kb on + strand at 3988.651 kb on + strand at 3988.652 kb on - strand at 3988.656 kb on - strand at 3988.703 kb on + strand, within BT3129 at 3988.713 kb on - strand, within BT3129 at 3988.797 kb on - strand, within BT3129 at 3988.841 kb on + strand, within BT3129 at 3988.841 kb on + strand, within BT3129 at 3988.842 kb on - strand, within BT3129 at 3988.844 kb on + strand, within BT3129 at 3988.845 kb on - strand, within BT3129 at 3988.905 kb on - strand, within BT3129 at 3989.010 kb on - strand, within BT3129 at 3989.052 kb on + strand, within BT3129 at 3989.052 kb on + strand, within BT3129 at 3989.053 kb on - strand, within BT3129 at 3989.106 kb on - strand, within BT3129 at 3989.171 kb on - strand, within BT3129 at 3989.195 kb on + strand, within BT3129 at 3989.264 kb on - strand, within BT3129 at 3989.268 kb on - strand, within BT3129 at 3989.284 kb on - strand, within BT3129 at 3989.284 kb on - strand, within BT3129 at 3989.306 kb on - strand, within BT3129 at 3989.306 kb on - strand, within BT3129 at 3989.328 kb on + strand, within BT3129 at 3989.328 kb on + strand, within BT3129 at 3989.330 kb on + strand, within BT3129 at 3989.330 kb on + strand, within BT3129 at 3989.330 kb on + strand, within BT3129 at 3989.330 kb on + strand, within BT3129 at 3989.331 kb on - strand, within BT3129 at 3989.331 kb on - strand, within BT3129
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.1 mM remove 3,986,868 - -0.8 3,986,878 + -0.2 3,986,885 + -2.6 3,986,886 - -1.3 3,986,895 + -0.3 3,986,895 + -0.7 3,986,949 + +0.5 3,986,953 + -0.8 3,986,977 + -1.8 3,987,031 - BT3127 0.14 -1.2 3,987,088 + BT3127 0.20 -1.5 3,987,132 - BT3127 0.25 +1.4 3,987,134 + BT3127 0.25 +0.8 3,987,134 + BT3127 0.25 -0.6 3,987,135 - BT3127 0.25 +0.8 3,987,163 + BT3127 0.28 +0.8 3,987,164 - BT3127 0.29 +0.5 3,987,212 + BT3127 0.34 +2.4 3,987,220 + BT3127 0.35 -2.6 3,987,224 + BT3127 0.35 -3.1 3,987,225 - BT3127 0.35 -1.2 3,987,295 - BT3127 0.43 -0.5 3,987,344 - BT3127 0.48 -0.0 3,987,560 + BT3127 0.72 -0.2 3,987,619 - BT3127 0.78 -0.1 3,987,717 + BT3127 0.89 -2.2 3,987,739 - -0.8 3,987,785 - -2.2 3,987,818 - +1.1 3,987,861 + -2.1 3,987,862 - +0.3 3,987,862 - -1.7 3,987,884 + +1.8 3,987,944 + BT3128 0.13 -1.8 3,987,945 - BT3128 0.13 -1.8 3,987,945 - BT3128 0.13 -1.2 3,988,042 + BT3128 0.30 -1.0 3,988,051 + BT3128 0.31 -2.2 3,988,052 - BT3128 0.32 +1.1 3,988,168 - BT3128 0.52 -0.8 3,988,325 - BT3128 0.79 -4.3 3,988,460 + -0.7 3,988,552 - +1.4 3,988,627 + +0.5 3,988,640 + -0.8 3,988,641 - -1.2 3,988,641 - -1.8 3,988,651 + +2.3 3,988,651 + -3.2 3,988,651 + -1.8 3,988,652 - -0.2 3,988,656 - +0.8 3,988,703 + BT3129 0.11 -1.8 3,988,713 - BT3129 0.12 -1.5 3,988,797 - BT3129 0.22 -1.6 3,988,841 + BT3129 0.27 -2.7 3,988,841 + BT3129 0.27 +0.3 3,988,842 - BT3129 0.27 +1.0 3,988,844 + BT3129 0.28 -1.2 3,988,845 - BT3129 0.28 +1.1 3,988,905 - BT3129 0.35 -0.5 3,989,010 - BT3129 0.47 +2.1 3,989,052 + BT3129 0.52 -1.6 3,989,052 + BT3129 0.52 -1.2 3,989,053 - BT3129 0.52 -1.2 3,989,106 - BT3129 0.59 +0.8 3,989,171 - BT3129 0.66 -0.8 3,989,195 + BT3129 0.69 -0.2 3,989,264 - BT3129 0.77 -1.9 3,989,268 - BT3129 0.78 +1.8 3,989,284 - BT3129 0.80 -1.9 3,989,284 - BT3129 0.80 +0.2 3,989,306 - BT3129 0.82 -1.1 3,989,306 - BT3129 0.82 -0.2 3,989,328 + BT3129 0.85 -1.4 3,989,328 + BT3129 0.85 -1.3 3,989,330 + BT3129 0.85 -1.5 3,989,330 + BT3129 0.85 +0.7 3,989,330 + BT3129 0.85 +1.4 3,989,330 + BT3129 0.85 -1.9 3,989,331 - BT3129 0.85 -0.0 3,989,331 - BT3129 0.85 +1.7
Or see this region's nucleotide sequence