Experiment: BHIS with Sulfamonomethoxine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0067 and BT0068 are separated by 27 nucleotides BT0068 and BT0069 are separated by 284 nucleotides BT0069 and BT0070 are separated by 47 nucleotides BT0070 and BT0071 are separated by 303 nucleotides
BT0067: BT0067 - hypothetical protein (NCBI ptt file), at 65,813 to 66,064
BT0067
BT0068: BT0068 - hypothetical protein (NCBI ptt file), at 66,092 to 66,571
BT0068
BT0069: BT0069 - conserved hypothetical protein (NCBI ptt file), at 66,856 to 67,662
BT0069
BT0070: BT0070 - conserved hypothetical protein (NCBI ptt file), at 67,710 to 68,333
BT0070
BT0071: BT0071 - hypothetical protein (NCBI ptt file), at 68,637 to 69,134
BT0071
Position (kb) 66
67
68 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 65.994 kb on - strand, within BT0067 at 65.999 kb on + strand, within BT0067 at 66.066 kb on - strand at 66.136 kb on + strand at 66.255 kb on + strand, within BT0068 at 66.438 kb on - strand, within BT0068 at 66.474 kb on - strand, within BT0068 at 66.532 kb on + strand at 66.533 kb on - strand at 66.576 kb on + strand at 66.625 kb on - strand at 66.784 kb on + strand at 66.785 kb on - strand at 66.805 kb on + strand at 66.822 kb on - strand at 66.857 kb on + strand at 66.857 kb on + strand at 66.863 kb on + strand at 66.864 kb on - strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.895 kb on + strand at 66.898 kb on + strand at 66.904 kb on + strand at 66.936 kb on + strand at 66.936 kb on + strand at 66.951 kb on - strand, within BT0069 at 66.959 kb on - strand, within BT0069 at 66.991 kb on + strand, within BT0069 at 67.000 kb on + strand, within BT0069 at 67.001 kb on - strand, within BT0069 at 67.020 kb on + strand, within BT0069 at 67.043 kb on - strand, within BT0069 at 67.077 kb on - strand, within BT0069 at 67.102 kb on - strand, within BT0069 at 67.102 kb on - strand, within BT0069 at 67.103 kb on + strand, within BT0069 at 67.104 kb on - strand, within BT0069 at 67.112 kb on - strand, within BT0069 at 67.112 kb on - strand, within BT0069 at 67.209 kb on - strand, within BT0069 at 67.212 kb on + strand, within BT0069 at 67.258 kb on + strand, within BT0069 at 67.262 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.280 kb on - strand, within BT0069 at 67.281 kb on + strand, within BT0069 at 67.281 kb on + strand, within BT0069 at 67.284 kb on - strand, within BT0069 at 67.286 kb on + strand, within BT0069 at 67.417 kb on - strand, within BT0069 at 67.842 kb on + strand, within BT0070 at 67.864 kb on - strand, within BT0070 at 67.864 kb on - strand, within BT0070 at 67.869 kb on - strand, within BT0070 at 67.887 kb on - strand, within BT0070 at 67.902 kb on - strand, within BT0070 at 67.935 kb on - strand, within BT0070 at 68.024 kb on + strand, within BT0070 at 68.066 kb on + strand, within BT0070 at 68.070 kb on - strand, within BT0070 at 68.116 kb on - strand, within BT0070 at 68.131 kb on + strand, within BT0070 at 68.157 kb on - strand, within BT0070 at 68.193 kb on - strand, within BT0070 at 68.219 kb on + strand, within BT0070 at 68.270 kb on + strand, within BT0070 at 68.272 kb on + strand at 68.273 kb on - strand at 68.273 kb on - strand at 68.326 kb on + strand at 68.327 kb on - strand at 68.346 kb on - strand at 68.365 kb on + strand at 68.616 kb on - strand at 68.651 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamonomethoxine 0.1 mM remove 65,994 - BT0067 0.72 -3.6 65,999 + BT0067 0.74 -0.5 66,066 - +0.1 66,136 + -1.5 66,255 + BT0068 0.34 +1.1 66,438 - BT0068 0.72 +0.1 66,474 - BT0068 0.80 -1.1 66,532 + -0.3 66,533 - +1.1 66,576 + -0.5 66,625 - -3.1 66,784 + -0.9 66,785 - +0.4 66,805 + +0.5 66,822 - -0.2 66,857 + -1.7 66,857 + -1.2 66,863 + -1.5 66,864 - -0.2 66,895 + -0.9 66,895 + -0.1 66,895 + -0.9 66,895 + +0.1 66,898 + +1.1 66,904 + -0.4 66,936 + -1.0 66,936 + +0.1 66,951 - BT0069 0.12 -4.2 66,959 - BT0069 0.13 -1.5 66,991 + BT0069 0.17 -1.3 67,000 + BT0069 0.18 -2.9 67,001 - BT0069 0.18 +1.7 67,020 + BT0069 0.20 -0.9 67,043 - BT0069 0.23 +1.1 67,077 - BT0069 0.27 -2.1 67,102 - BT0069 0.30 -1.7 67,102 - BT0069 0.30 -0.3 67,103 + BT0069 0.31 -0.5 67,104 - BT0069 0.31 +0.7 67,112 - BT0069 0.32 -1.7 67,112 - BT0069 0.32 -2.8 67,209 - BT0069 0.44 +1.1 67,212 + BT0069 0.44 -2.5 67,258 + BT0069 0.50 -2.8 67,262 - BT0069 0.50 +1.3 67,280 - BT0069 0.53 -2.7 67,280 - BT0069 0.53 -2.2 67,280 - BT0069 0.53 -1.9 67,281 + BT0069 0.53 -1.3 67,281 + BT0069 0.53 -0.9 67,284 - BT0069 0.53 +1.7 67,286 + BT0069 0.53 -0.9 67,417 - BT0069 0.70 -2.2 67,842 + BT0070 0.21 -2.2 67,864 - BT0070 0.25 -1.1 67,864 - BT0070 0.25 -2.8 67,869 - BT0070 0.25 -1.5 67,887 - BT0070 0.28 -0.9 67,902 - BT0070 0.31 -0.9 67,935 - BT0070 0.36 -1.1 68,024 + BT0070 0.50 -1.2 68,066 + BT0070 0.57 -1.5 68,070 - BT0070 0.58 +0.1 68,116 - BT0070 0.65 +0.1 68,131 + BT0070 0.67 -3.4 68,157 - BT0070 0.72 +1.1 68,193 - BT0070 0.77 -0.5 68,219 + BT0070 0.82 -0.9 68,270 + BT0070 0.90 -0.3 68,272 + +0.1 68,273 - -0.9 68,273 - -1.9 68,326 + -1.5 68,327 - -2.2 68,346 - -1.5 68,365 + +0.3 68,616 - +0.3 68,651 - +1.7
Or see this region's nucleotide sequence