Experiment: KB with Solanesol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4682 and Psyr_4683 are separated by 153 nucleotides Psyr_4683 and Psyr_4684 are separated by 24 nucleotides Psyr_4684 and Psyr_4685 overlap by 8 nucleotides Psyr_4685 and Psyr_4686 overlap by 8 nucleotides
Psyr_4682: Psyr_4682 - conserved hypothetical protein, at 5,550,906 to 5,551,202
_4682
Psyr_4683: Psyr_4683 - dethiobiotin synthase, at 5,551,356 to 5,552,036
_4683
Psyr_4684: Psyr_4684 - biotin synthesis protein BioC, at 5,552,061 to 5,552,870
_4684
Psyr_4685: Psyr_4685 - bioH protein, at 5,552,863 to 5,553,594
_4685
Psyr_4686: Psyr_4686 - 8-amino-7-oxononanoate synthase, at 5,553,587 to 5,554,777
_4686
Position (kb) 5552
5553 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5551.202 kb on + strand at 5551.202 kb on + strand at 5551.263 kb on - strand at 5551.278 kb on + strand at 5551.345 kb on + strand at 5551.381 kb on + strand at 5551.382 kb on - strand at 5551.530 kb on + strand, within Psyr_4683 at 5551.530 kb on + strand, within Psyr_4683 at 5551.531 kb on - strand, within Psyr_4683 at 5551.615 kb on - strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.831 kb on + strand, within Psyr_4683 at 5552.083 kb on + strand at 5552.084 kb on - strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.134 kb on + strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.392 kb on + strand, within Psyr_4684 at 5552.392 kb on + strand, within Psyr_4684 at 5552.393 kb on - strand, within Psyr_4684 at 5552.630 kb on + strand, within Psyr_4684 at 5552.630 kb on + strand, within Psyr_4684 at 5552.631 kb on - strand, within Psyr_4684 at 5552.782 kb on + strand, within Psyr_4684 at 5553.060 kb on - strand, within Psyr_4685 at 5553.097 kb on + strand, within Psyr_4685 at 5553.098 kb on - strand, within Psyr_4685 at 5553.656 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.660 kb on - strand at 5553.660 kb on - strand at 5553.660 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Solanesol 10 mM remove 5,551,202 + +0.4 5,551,202 + -0.5 5,551,263 - +1.9 5,551,278 + -0.5 5,551,345 + +0.6 5,551,381 + -1.3 5,551,382 - -1.6 5,551,530 + Psyr_4683 0.26 -3.3 5,551,530 + Psyr_4683 0.26 -3.7 5,551,531 - Psyr_4683 0.26 -3.1 5,551,615 - Psyr_4683 0.38 -1.2 5,551,721 + Psyr_4683 0.54 -2.3 5,551,721 + Psyr_4683 0.54 -2.9 5,551,721 + Psyr_4683 0.54 -0.6 5,551,721 + Psyr_4683 0.54 -1.2 5,551,721 + Psyr_4683 0.54 -1.7 5,551,721 + Psyr_4683 0.54 -2.0 5,551,722 - Psyr_4683 0.54 -3.4 5,551,722 - Psyr_4683 0.54 -1.7 5,551,722 - Psyr_4683 0.54 -1.8 5,551,722 - Psyr_4683 0.54 -0.3 5,551,722 - Psyr_4683 0.54 -1.5 5,551,831 + Psyr_4683 0.70 -0.2 5,552,083 + -2.5 5,552,084 - -1.8 5,552,095 + -2.4 5,552,095 + +1.0 5,552,095 + -2.9 5,552,095 + +1.2 5,552,096 - -0.6 5,552,096 - -0.8 5,552,096 - -1.3 5,552,096 - -0.6 5,552,134 + +1.2 5,552,135 - -2.3 5,552,135 - -2.7 5,552,135 - +1.2 5,552,135 - -3.0 5,552,392 + Psyr_4684 0.41 -2.2 5,552,392 + Psyr_4684 0.41 -1.2 5,552,393 - Psyr_4684 0.41 -0.5 5,552,630 + Psyr_4684 0.70 -1.4 5,552,630 + Psyr_4684 0.70 -1.1 5,552,631 - Psyr_4684 0.70 -3.8 5,552,782 + Psyr_4684 0.89 +0.5 5,553,060 - Psyr_4685 0.27 -1.9 5,553,097 + Psyr_4685 0.32 -1.7 5,553,098 - Psyr_4685 0.32 +0.6 5,553,656 + -2.9 5,553,659 + -1.3 5,553,659 + -1.8 5,553,659 + -1.2 5,553,659 + -0.7 5,553,660 - -0.8 5,553,660 - -0.3 5,553,660 - -3.1
Or see this region's nucleotide sequence