Experiment: Formate/Acetate-Sulfite (60/30-60 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpA and sdhB are separated by 1 nucleotides sdhB and DVU2675 are separated by 221 nucleotides DVU2675 and DVU2676 are separated by 250 nucleotides DVU2676 and DVU2677 are separated by 127 nucleotides
DVU2673: glpA - anaerobic glycerol-3-phosphate dehydrogenase, subunit A, truncation (TIGR), at 2,784,563 to 2,785,732
glpA
DVU2674: sdhB - succinate dehydrogenase and fumarate reductase iron-sulfur protein (TIGR), at 2,785,734 to 2,786,453
sdhB
DVU2675: DVU2675 - DNA-binding response regulator, LuxR family (TIGR), at 2,786,675 to 2,787,358
DVU2675
DVU2676: DVU2676 - hypothetical protein (TIGR), at 2,787,609 to 2,787,875
DVU2676
DVU2677: DVU2677 - sensor histidine kinase/response regulator (TIGR), at 2,788,003 to 2,789,466
DVU2677
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2785.686 kb on + strand at 2785.707 kb on - strand at 2785.754 kb on + strand at 2786.026 kb on + strand, within sdhB at 2786.032 kb on - strand, within sdhB at 2786.050 kb on + strand, within sdhB at 2786.199 kb on + strand, within sdhB at 2786.207 kb on - strand, within sdhB at 2786.304 kb on - strand, within sdhB at 2786.338 kb on + strand, within sdhB at 2786.341 kb on + strand, within sdhB at 2786.364 kb on + strand, within sdhB at 2786.375 kb on + strand, within sdhB at 2786.384 kb on - strand at 2786.429 kb on - strand at 2786.453 kb on + strand at 2786.495 kb on - strand at 2786.561 kb on - strand at 2786.608 kb on + strand at 2786.691 kb on + strand at 2786.741 kb on + strand at 2786.751 kb on + strand, within DVU2675 at 2786.759 kb on - strand, within DVU2675 at 2786.764 kb on - strand, within DVU2675 at 2786.817 kb on + strand, within DVU2675 at 2786.864 kb on + strand, within DVU2675 at 2786.864 kb on + strand, within DVU2675 at 2786.881 kb on + strand, within DVU2675 at 2786.918 kb on + strand, within DVU2675 at 2786.936 kb on - strand, within DVU2675 at 2786.941 kb on - strand, within DVU2675 at 2787.018 kb on - strand, within DVU2675 at 2787.022 kb on + strand, within DVU2675 at 2787.030 kb on - strand, within DVU2675 at 2787.137 kb on + strand, within DVU2675 at 2787.138 kb on + strand, within DVU2675 at 2787.202 kb on + strand, within DVU2675 at 2787.216 kb on + strand, within DVU2675 at 2787.216 kb on + strand, within DVU2675 at 2787.218 kb on + strand, within DVU2675 at 2787.224 kb on - strand, within DVU2675 at 2787.234 kb on - strand, within DVU2675 at 2787.234 kb on - strand, within DVU2675 at 2787.352 kb on + strand at 2787.360 kb on - strand at 2787.398 kb on + strand at 2787.398 kb on + strand at 2787.406 kb on - strand at 2787.718 kb on - strand, within DVU2676 at 2787.744 kb on - strand, within DVU2676 at 2787.787 kb on + strand, within DVU2676 at 2787.795 kb on - strand, within DVU2676 at 2787.798 kb on + strand, within DVU2676 at 2787.832 kb on - strand, within DVU2676 at 2787.846 kb on + strand, within DVU2676 at 2787.971 kb on - strand at 2787.996 kb on - strand at 2788.001 kb on - strand at 2788.027 kb on + strand at 2788.040 kb on + strand at 2788.040 kb on + strand at 2788.040 kb on + strand at 2788.048 kb on - strand at 2788.048 kb on - strand at 2788.198 kb on + strand, within DVU2677 at 2788.242 kb on + strand, within DVU2677 at 2788.270 kb on + strand, within DVU2677 at 2788.277 kb on + strand, within DVU2677 at 2788.286 kb on - strand, within DVU2677
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfite (60/30-60 mM) + 0.1% Yeast Extract remove 2,785,686 + +0.5 2,785,707 - +0.3 2,785,754 + -0.2 2,786,026 + sdhB DVU2674 0.41 -1.0 2,786,032 - sdhB DVU2674 0.41 -0.5 2,786,050 + sdhB DVU2674 0.44 -0.1 2,786,199 + sdhB DVU2674 0.65 -0.1 2,786,207 - sdhB DVU2674 0.66 +1.3 2,786,304 - sdhB DVU2674 0.79 -0.1 2,786,338 + sdhB DVU2674 0.84 +2.1 2,786,341 + sdhB DVU2674 0.84 +0.4 2,786,364 + sdhB DVU2674 0.88 +0.1 2,786,375 + sdhB DVU2674 0.89 -0.1 2,786,384 - +0.1 2,786,429 - -0.2 2,786,453 + -0.2 2,786,495 - -0.2 2,786,561 - -0.0 2,786,608 + +0.5 2,786,691 + +0.4 2,786,741 + +1.2 2,786,751 + DVU2675 0.11 -1.2 2,786,759 - DVU2675 0.12 -1.3 2,786,764 - DVU2675 0.13 -0.5 2,786,817 + DVU2675 0.21 -1.4 2,786,864 + DVU2675 0.28 -0.5 2,786,864 + DVU2675 0.28 -0.4 2,786,881 + DVU2675 0.30 +0.1 2,786,918 + DVU2675 0.36 -0.4 2,786,936 - DVU2675 0.38 -1.5 2,786,941 - DVU2675 0.39 +0.1 2,787,018 - DVU2675 0.50 -0.0 2,787,022 + DVU2675 0.51 -0.6 2,787,030 - DVU2675 0.52 -0.5 2,787,137 + DVU2675 0.68 -0.9 2,787,138 + DVU2675 0.68 -0.5 2,787,202 + DVU2675 0.77 -1.7 2,787,216 + DVU2675 0.79 -0.1 2,787,216 + DVU2675 0.79 +0.3 2,787,218 + DVU2675 0.79 -0.3 2,787,224 - DVU2675 0.80 -0.1 2,787,234 - DVU2675 0.82 -0.2 2,787,234 - DVU2675 0.82 -0.6 2,787,352 + -0.3 2,787,360 - -0.2 2,787,398 + +0.5 2,787,398 + +0.5 2,787,406 - +0.1 2,787,718 - DVU2676 0.41 -0.1 2,787,744 - DVU2676 0.51 -0.5 2,787,787 + DVU2676 0.67 -1.4 2,787,795 - DVU2676 0.70 +0.1 2,787,798 + DVU2676 0.71 +0.1 2,787,832 - DVU2676 0.84 -2.0 2,787,846 + DVU2676 0.89 -0.2 2,787,971 - -0.0 2,787,996 - -0.3 2,788,001 - -0.7 2,788,027 + +0.6 2,788,040 + +0.4 2,788,040 + +0.0 2,788,040 + +0.4 2,788,048 - -0.1 2,788,048 - -0.1 2,788,198 + DVU2677 0.13 +0.0 2,788,242 + DVU2677 0.16 +0.4 2,788,270 + DVU2677 0.18 -0.0 2,788,277 + DVU2677 0.19 -0.0 2,788,286 - DVU2677 0.19 +0.7
Or see this region's nucleotide sequence