Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1366 and pheA are separated by 94 nucleotides pheA and pepA-2 are separated by 370 nucleotides
SO1366: SO1366 - sodium/hydrogen exchanger family protein (NCBI ptt file), at 1,421,554 to 1,422,858
SO1366
SO1367: pheA - chorismate mutase/prephenate dehydratase (NCBI ptt file), at 1,422,953 to 1,424,968
pheA
SO1368: pepA-2 - cytosol aminopeptidase (NCBI ptt file), at 1,425,339 to 1,426,847
pepA-2
Position (kb)
1422
1423
1424
1425 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1422.016 kb on + strand, within SO1366 at 1422.031 kb on + strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.039 kb on - strand, within SO1366 at 1422.090 kb on + strand, within SO1366 at 1422.185 kb on - strand, within SO1366 at 1422.192 kb on + strand, within SO1366 at 1422.195 kb on - strand, within SO1366 at 1422.407 kb on - strand, within SO1366 at 1422.458 kb on - strand, within SO1366 at 1422.517 kb on - strand, within SO1366 at 1422.586 kb on - strand, within SO1366 at 1422.715 kb on - strand, within SO1366 at 1422.715 kb on - strand, within SO1366 at 1422.851 kb on + strand at 1423.013 kb on - strand at 1423.047 kb on + strand at 1423.055 kb on - strand at 1423.057 kb on + strand at 1423.140 kb on + strand at 1423.148 kb on - strand at 1423.150 kb on + strand at 1423.150 kb on + strand at 1423.158 kb on - strand, within pheA at 1423.158 kb on - strand, within pheA at 1423.166 kb on + strand, within pheA at 1423.166 kb on + strand, within pheA at 1423.323 kb on + strand, within pheA at 1423.390 kb on + strand, within pheA at 1423.471 kb on + strand, within pheA at 1423.471 kb on + strand, within pheA at 1423.479 kb on - strand, within pheA at 1423.655 kb on + strand, within pheA at 1423.663 kb on - strand, within pheA at 1423.718 kb on - strand, within pheA at 1423.741 kb on + strand, within pheA at 1423.741 kb on + strand, within pheA at 1423.816 kb on + strand, within pheA at 1423.816 kb on + strand, within pheA at 1423.824 kb on - strand, within pheA at 1423.824 kb on - strand, within pheA at 1423.855 kb on - strand, within pheA at 1423.877 kb on + strand, within pheA at 1423.959 kb on + strand, within pheA at 1424.033 kb on - strand, within pheA at 1424.158 kb on + strand, within pheA at 1424.205 kb on - strand, within pheA at 1424.257 kb on + strand, within pheA at 1424.278 kb on + strand, within pheA at 1424.292 kb on + strand, within pheA at 1424.293 kb on - strand, within pheA at 1424.325 kb on + strand, within pheA at 1424.352 kb on + strand, within pheA at 1424.352 kb on + strand, within pheA at 1424.360 kb on - strand, within pheA at 1424.362 kb on + strand, within pheA at 1424.453 kb on + strand, within pheA at 1424.453 kb on + strand, within pheA at 1424.461 kb on - strand, within pheA at 1424.649 kb on + strand, within pheA at 1424.816 kb on + strand at 1424.840 kb on + strand at 1424.866 kb on + strand at 1425.023 kb on + strand at 1425.130 kb on + strand at 1425.138 kb on - strand at 1425.143 kb on + strand at 1425.370 kb on + strand at 1425.424 kb on + strand at 1425.625 kb on + strand, within pepA-2 at 1425.633 kb on - strand, within pepA-2 at 1425.635 kb on + strand, within pepA-2 at 1425.638 kb on - strand, within pepA-2 at 1425.671 kb on - strand, within pepA-2 at 1425.703 kb on + strand, within pepA-2 at 1425.713 kb on + strand, within pepA-2 at 1425.713 kb on + strand, within pepA-2 at 1425.721 kb on - strand, within pepA-2 at 1425.767 kb on - strand, within pepA-2 at 1425.809 kb on + strand, within pepA-2 at 1425.859 kb on + strand, within pepA-2 at 1425.859 kb on + strand, within pepA-2 at 1425.893 kb on - strand, within pepA-2 at 1425.948 kb on + strand, within pepA-2
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 1,422,016 + SO1366 0.35 -0.7 1,422,031 + SO1366 0.37 +0.3 1,422,039 - SO1366 0.37 +1.9 1,422,039 - SO1366 0.37 +0.5 1,422,039 - SO1366 0.37 +0.1 1,422,090 + SO1366 0.41 +0.8 1,422,185 - SO1366 0.48 +0.9 1,422,192 + SO1366 0.49 +0.8 1,422,195 - SO1366 0.49 +0.2 1,422,407 - SO1366 0.65 +0.1 1,422,458 - SO1366 0.69 +0.5 1,422,517 - SO1366 0.74 +1.0 1,422,586 - SO1366 0.79 -0.8 1,422,715 - SO1366 0.89 -0.8 1,422,715 - SO1366 0.89 -0.9 1,422,851 + +0.8 1,423,013 - +0.9 1,423,047 + -1.2 1,423,055 - -1.1 1,423,057 + -0.7 1,423,140 + -0.5 1,423,148 - -0.4 1,423,150 + -1.1 1,423,150 + -0.2 1,423,158 - pheA SO1367 0.10 -0.1 1,423,158 - pheA SO1367 0.10 -0.5 1,423,166 + pheA SO1367 0.11 -1.5 1,423,166 + pheA SO1367 0.11 -3.1 1,423,323 + pheA SO1367 0.18 -1.1 1,423,390 + pheA SO1367 0.22 -2.4 1,423,471 + pheA SO1367 0.26 -2.1 1,423,471 + pheA SO1367 0.26 -0.1 1,423,479 - pheA SO1367 0.26 -2.2 1,423,655 + pheA SO1367 0.35 -2.4 1,423,663 - pheA SO1367 0.35 -2.2 1,423,718 - pheA SO1367 0.38 -1.0 1,423,741 + pheA SO1367 0.39 +1.2 1,423,741 + pheA SO1367 0.39 -0.9 1,423,816 + pheA SO1367 0.43 +0.6 1,423,816 + pheA SO1367 0.43 -0.7 1,423,824 - pheA SO1367 0.43 +0.2 1,423,824 - pheA SO1367 0.43 -0.5 1,423,855 - pheA SO1367 0.45 -4.7 1,423,877 + pheA SO1367 0.46 -5.0 1,423,959 + pheA SO1367 0.50 -4.9 1,424,033 - pheA SO1367 0.54 -1.1 1,424,158 + pheA SO1367 0.60 -3.7 1,424,205 - pheA SO1367 0.62 -4.2 1,424,257 + pheA SO1367 0.65 -2.4 1,424,278 + pheA SO1367 0.66 -1.1 1,424,292 + pheA SO1367 0.66 -1.1 1,424,293 - pheA SO1367 0.66 -3.3 1,424,325 + pheA SO1367 0.68 -3.4 1,424,352 + pheA SO1367 0.69 -4.9 1,424,352 + pheA SO1367 0.69 -2.7 1,424,360 - pheA SO1367 0.70 -3.4 1,424,362 + pheA SO1367 0.70 -3.6 1,424,453 + pheA SO1367 0.74 -1.1 1,424,453 + pheA SO1367 0.74 -2.4 1,424,461 - pheA SO1367 0.75 -4.6 1,424,649 + pheA SO1367 0.84 -4.6 1,424,816 + -4.5 1,424,840 + -5.2 1,424,866 + -3.3 1,425,023 + -4.4 1,425,130 + -3.8 1,425,138 - -1.4 1,425,143 + -3.4 1,425,370 + -2.1 1,425,424 + -0.9 1,425,625 + pepA-2 SO1368 0.19 -0.1 1,425,633 - pepA-2 SO1368 0.19 -0.1 1,425,635 + pepA-2 SO1368 0.20 -1.4 1,425,638 - pepA-2 SO1368 0.20 -2.7 1,425,671 - pepA-2 SO1368 0.22 -2.4 1,425,703 + pepA-2 SO1368 0.24 -1.2 1,425,713 + pepA-2 SO1368 0.25 -0.1 1,425,713 + pepA-2 SO1368 0.25 -1.1 1,425,721 - pepA-2 SO1368 0.25 -2.1 1,425,767 - pepA-2 SO1368 0.28 -1.1 1,425,809 + pepA-2 SO1368 0.31 -2.0 1,425,859 + pepA-2 SO1368 0.34 -1.7 1,425,859 + pepA-2 SO1368 0.34 -1.1 1,425,893 - pepA-2 SO1368 0.37 -1.5 1,425,948 + pepA-2 SO1368 0.40 -1.4
Or see this region's nucleotide sequence