Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmD and rplS are separated by 34 nucleotides rplS and aroF are separated by 296 nucleotides aroF and tyrA are separated by 62 nucleotides
SO1359: trmD - tRNA (guanine-N1)-methyltransferase (NCBI ptt file), at 1,414,251 to 1,414,997
trmD
SO1360: rplS - ribosomal protein L19 (NCBI ptt file), at 1,415,032 to 1,415,385
rplS
SO1361: aroF - phospho-2-dehydro-3-deoxyheptonate aldolase, tyr-sensitive (NCBI ptt file), at 1,415,682 to 1,416,773
aroF
SO1362: tyrA - chorismate mutase/prephenate dehydrogenase (NCBI ptt file), at 1,416,836 to 1,417,975
tyrA
Position (kb)
1415
1416
1417 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1415.680 kb on + strand at 1415.984 kb on - strand, within aroF at 1416.049 kb on + strand, within aroF at 1416.122 kb on - strand, within aroF at 1416.133 kb on - strand, within aroF at 1416.172 kb on - strand, within aroF at 1416.204 kb on - strand, within aroF at 1416.329 kb on + strand, within aroF at 1416.344 kb on + strand, within aroF at 1416.361 kb on + strand, within aroF at 1416.370 kb on - strand, within aroF at 1416.391 kb on + strand, within aroF at 1416.513 kb on + strand, within aroF at 1416.513 kb on + strand, within aroF at 1416.548 kb on + strand, within aroF at 1416.712 kb on + strand at 1416.725 kb on - strand at 1416.809 kb on + strand at 1416.953 kb on + strand, within tyrA at 1416.959 kb on - strand, within tyrA at 1417.084 kb on + strand, within tyrA at 1417.169 kb on + strand, within tyrA at 1417.304 kb on + strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.628 kb on + strand, within tyrA at 1417.641 kb on + strand, within tyrA at 1417.641 kb on + strand, within tyrA at 1417.753 kb on + strand, within tyrA
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 1,415,680 + -1.3 1,415,984 - aroF SO1361 0.28 -2.4 1,416,049 + aroF SO1361 0.34 -0.7 1,416,122 - aroF SO1361 0.40 -1.9 1,416,133 - aroF SO1361 0.41 -1.7 1,416,172 - aroF SO1361 0.45 -3.3 1,416,204 - aroF SO1361 0.48 +0.9 1,416,329 + aroF SO1361 0.59 +0.5 1,416,344 + aroF SO1361 0.61 -1.1 1,416,361 + aroF SO1361 0.62 -0.6 1,416,370 - aroF SO1361 0.63 -3.3 1,416,391 + aroF SO1361 0.65 -0.8 1,416,513 + aroF SO1361 0.76 -0.1 1,416,513 + aroF SO1361 0.76 -1.2 1,416,548 + aroF SO1361 0.79 +0.3 1,416,712 + -0.1 1,416,725 - -4.6 1,416,809 + -2.6 1,416,953 + tyrA SO1362 0.10 -2.9 1,416,959 - tyrA SO1362 0.11 -2.1 1,417,084 + tyrA SO1362 0.22 -1.1 1,417,169 + tyrA SO1362 0.29 -2.7 1,417,304 + tyrA SO1362 0.41 -2.1 1,417,488 - tyrA SO1362 0.57 -1.1 1,417,488 - tyrA SO1362 0.57 -0.1 1,417,488 - tyrA SO1362 0.57 -5.1 1,417,628 + tyrA SO1362 0.69 -3.6 1,417,641 + tyrA SO1362 0.71 -4.0 1,417,641 + tyrA SO1362 0.71 -3.1 1,417,753 + tyrA SO1362 0.80 -3.4
Or see this region's nucleotide sequence