Experiment: 20 mM D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rseB and rseC are separated by 11 nucleotides rseC and lepA are separated by 167 nucleotides lepA and lepB are separated by 178 nucleotides
SO1344: rseB - sigma-E factor regulatory protein RseB (NCBI ptt file), at 1,400,453 to 1,401,385
rseB
SO1345: rseC - sigma-E factor regulatory protein RseC (NCBI ptt file), at 1,401,397 to 1,401,870
rseC
SO1346: lepA - GTP-binding protein LepA (NCBI ptt file), at 1,402,038 to 1,403,828
lepA
SO1347: lepB - signal peptidase I (NCBI ptt file), at 1,404,007 to 1,404,924
lepB
Position (kb)
1402
1403
1404 Strain fitness (log2 ratio)
-1
0
1
2 at 1401.235 kb on + strand, within rseB at 1401.278 kb on + strand, within rseB at 1401.935 kb on + strand at 1401.994 kb on + strand at 1402.134 kb on - strand at 1402.382 kb on + strand, within lepA at 1402.502 kb on + strand, within lepA at 1402.711 kb on - strand, within lepA at 1402.912 kb on + strand, within lepA at 1403.034 kb on + strand, within lepA at 1403.034 kb on + strand, within lepA at 1403.067 kb on + strand, within lepA at 1403.628 kb on + strand, within lepA at 1403.693 kb on + strand at 1403.693 kb on + strand at 1403.878 kb on + strand at 1404.156 kb on + strand, within lepB at 1404.445 kb on + strand, within lepB at 1404.467 kb on + strand, within lepB at 1404.559 kb on + strand, within lepB at 1404.608 kb on + strand, within lepB at 1404.764 kb on + strand, within lepB
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM D,L-Lactate remove 1,401,235 + rseB SO1344 0.84 +2.2 1,401,278 + rseB SO1344 0.88 +2.0 1,401,935 + -1.7 1,401,994 + -1.1 1,402,134 - -0.1 1,402,382 + lepA SO1346 0.19 -0.1 1,402,502 + lepA SO1346 0.26 -1.7 1,402,711 - lepA SO1346 0.38 -0.1 1,402,912 + lepA SO1346 0.49 -1.1 1,403,034 + lepA SO1346 0.56 -1.7 1,403,034 + lepA SO1346 0.56 -1.1 1,403,067 + lepA SO1346 0.57 -1.1 1,403,628 + lepA SO1346 0.89 -0.1 1,403,693 + -1.1 1,403,693 + -0.1 1,403,878 + +0.2 1,404,156 + lepB SO1347 0.16 +0.5 1,404,445 + lepB SO1347 0.48 -0.7 1,404,467 + lepB SO1347 0.50 +2.5 1,404,559 + lepB SO1347 0.60 +2.5 1,404,608 + lepB SO1347 0.65 +1.9 1,404,764 + lepB SO1347 0.82 +1.1
Or see this region's nucleotide sequence