Experiment: KB with Biochanin a diacetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2993 and Psyr_2994 overlap by 11 nucleotides Psyr_2994 and Psyr_2995 overlap by 4 nucleotides
Psyr_2993: Psyr_2993 - maltooligosyl trehalose hydrolase, at 3,585,283 to 3,587,097
_2993
Psyr_2994: Psyr_2994 - Glycoside hydrolase, family 77, at 3,587,087 to 3,589,165
_2994
Psyr_2995: Psyr_2995 - maltooligosyl trehalose synthase, at 3,589,162 to 3,591,945
_2995
Position (kb)
3587
3588
3589
3590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3586.264 kb on + strand, within Psyr_2993 at 3586.267 kb on + strand, within Psyr_2993 at 3586.268 kb on - strand, within Psyr_2993 at 3586.268 kb on - strand, within Psyr_2993 at 3586.330 kb on + strand, within Psyr_2993 at 3586.331 kb on - strand, within Psyr_2993 at 3586.359 kb on + strand, within Psyr_2993 at 3586.360 kb on - strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.805 kb on + strand, within Psyr_2993 at 3586.982 kb on + strand at 3586.982 kb on + strand at 3586.983 kb on - strand at 3586.983 kb on - strand at 3587.038 kb on + strand at 3587.038 kb on + strand at 3587.039 kb on - strand at 3587.197 kb on - strand at 3587.365 kb on - strand, within Psyr_2994 at 3587.365 kb on - strand, within Psyr_2994 at 3587.564 kb on + strand, within Psyr_2994 at 3587.705 kb on + strand, within Psyr_2994 at 3587.903 kb on + strand, within Psyr_2994 at 3588.153 kb on + strand, within Psyr_2994 at 3588.154 kb on - strand, within Psyr_2994 at 3588.509 kb on + strand, within Psyr_2994 at 3588.510 kb on - strand, within Psyr_2994 at 3588.855 kb on - strand, within Psyr_2994 at 3588.947 kb on + strand, within Psyr_2994 at 3588.948 kb on - strand, within Psyr_2994 at 3588.958 kb on + strand at 3588.958 kb on + strand at 3588.959 kb on - strand at 3588.959 kb on - strand at 3588.975 kb on + strand at 3589.122 kb on + strand at 3589.123 kb on - strand at 3589.123 kb on - strand at 3589.123 kb on - strand at 3589.176 kb on - strand at 3589.176 kb on - strand at 3589.252 kb on + strand at 3589.516 kb on + strand, within Psyr_2995 at 3589.562 kb on + strand, within Psyr_2995 at 3589.562 kb on + strand, within Psyr_2995 at 3589.563 kb on - strand, within Psyr_2995 at 3589.600 kb on + strand, within Psyr_2995 at 3589.663 kb on + strand, within Psyr_2995 at 3589.675 kb on + strand, within Psyr_2995 at 3589.708 kb on + strand, within Psyr_2995 at 3589.708 kb on + strand, within Psyr_2995 at 3589.709 kb on - strand, within Psyr_2995 at 3589.796 kb on - strand, within Psyr_2995 at 3589.796 kb on - strand, within Psyr_2995 at 3589.796 kb on - strand, within Psyr_2995 at 3589.796 kb on - strand, within Psyr_2995 at 3590.045 kb on + strand, within Psyr_2995 at 3590.045 kb on + strand, within Psyr_2995 at 3590.046 kb on - strand, within Psyr_2995 at 3590.046 kb on - strand, within Psyr_2995 at 3590.069 kb on + strand, within Psyr_2995
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Biochanin a diacetate 10 mM remove 3,586,264 + Psyr_2993 0.54 +0.0 3,586,267 + Psyr_2993 0.54 -1.0 3,586,268 - Psyr_2993 0.54 -3.2 3,586,268 - Psyr_2993 0.54 -0.4 3,586,330 + Psyr_2993 0.58 -0.6 3,586,331 - Psyr_2993 0.58 -0.6 3,586,359 + Psyr_2993 0.59 +0.8 3,586,360 - Psyr_2993 0.59 +0.3 3,586,754 + Psyr_2993 0.81 -1.2 3,586,754 + Psyr_2993 0.81 -0.6 3,586,754 + Psyr_2993 0.81 +0.3 3,586,754 + Psyr_2993 0.81 -0.5 3,586,754 + Psyr_2993 0.81 +1.9 3,586,754 + Psyr_2993 0.81 -1.5 3,586,754 + Psyr_2993 0.81 +1.0 3,586,754 + Psyr_2993 0.81 -0.3 3,586,754 + Psyr_2993 0.81 -1.0 3,586,755 - Psyr_2993 0.81 -0.9 3,586,755 - Psyr_2993 0.81 -0.6 3,586,755 - Psyr_2993 0.81 -0.6 3,586,805 + Psyr_2993 0.84 -1.4 3,586,982 + -0.4 3,586,982 + -1.6 3,586,983 - -0.6 3,586,983 - +0.0 3,587,038 + +0.4 3,587,038 + +0.3 3,587,039 - +0.5 3,587,197 - -0.3 3,587,365 - Psyr_2994 0.13 -1.0 3,587,365 - Psyr_2994 0.13 -1.0 3,587,564 + Psyr_2994 0.23 +0.4 3,587,705 + Psyr_2994 0.30 -1.6 3,587,903 + Psyr_2994 0.39 +0.6 3,588,153 + Psyr_2994 0.51 -1.2 3,588,154 - Psyr_2994 0.51 +0.0 3,588,509 + Psyr_2994 0.68 -1.1 3,588,510 - Psyr_2994 0.68 -1.3 3,588,855 - Psyr_2994 0.85 -0.2 3,588,947 + Psyr_2994 0.89 -0.6 3,588,948 - Psyr_2994 0.90 -0.7 3,588,958 + -1.3 3,588,958 + +0.2 3,588,959 - -0.0 3,588,959 - -0.4 3,588,975 + -0.3 3,589,122 + +1.1 3,589,123 - +0.6 3,589,123 - -2.0 3,589,123 - +1.0 3,589,176 - +1.0 3,589,176 - -0.2 3,589,252 + -0.2 3,589,516 + Psyr_2995 0.13 -0.3 3,589,562 + Psyr_2995 0.14 -1.8 3,589,562 + Psyr_2995 0.14 +1.2 3,589,563 - Psyr_2995 0.14 -0.6 3,589,600 + Psyr_2995 0.16 +0.0 3,589,663 + Psyr_2995 0.18 -2.3 3,589,675 + Psyr_2995 0.18 -0.4 3,589,708 + Psyr_2995 0.20 -0.0 3,589,708 + Psyr_2995 0.20 +1.6 3,589,709 - Psyr_2995 0.20 -2.3 3,589,796 - Psyr_2995 0.23 -0.2 3,589,796 - Psyr_2995 0.23 +0.4 3,589,796 - Psyr_2995 0.23 +1.0 3,589,796 - Psyr_2995 0.23 -0.3 3,590,045 + Psyr_2995 0.32 -0.4 3,590,045 + Psyr_2995 0.32 -0.0 3,590,046 - Psyr_2995 0.32 -0.9 3,590,046 - Psyr_2995 0.32 +0.1 3,590,069 + Psyr_2995 0.33 -0.2
Or see this region's nucleotide sequence