Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_586 and HP15_587 are separated by 23 nucleotides HP15_587 and HP15_588 overlap by 4 nucleotides HP15_588 and HP15_589 are separated by 198 nucleotides
HP15_586: HP15_586 - thymidylate synthase, at 607,119 to 607,958
_586
HP15_587: HP15_587 - protein containing dihydrofolate reductase region domain, at 607,982 to 608,491
_587
HP15_588: HP15_588 - DEAD/DEAH box helicase domain protein, at 608,488 to 610,011
_588
HP15_589: HP15_589 - acyl-CoA thioesterase II, at 610,210 to 610,998
_589
Position (kb) 608
609
610
611 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 607.958 kb on + strand at 607.963 kb on + strand at 607.973 kb on + strand at 607.976 kb on + strand at 607.991 kb on + strand at 608.989 kb on + strand, within HP15_588 at 609.029 kb on - strand, within HP15_588 at 609.087 kb on + strand, within HP15_588 at 609.095 kb on - strand, within HP15_588 at 609.095 kb on - strand, within HP15_588 at 609.095 kb on - strand, within HP15_588 at 609.095 kb on - strand, within HP15_588 at 609.140 kb on + strand, within HP15_588 at 609.191 kb on - strand, within HP15_588 at 609.307 kb on + strand, within HP15_588 at 609.404 kb on - strand, within HP15_588 at 609.426 kb on - strand, within HP15_588 at 609.426 kb on - strand, within HP15_588 at 609.634 kb on + strand, within HP15_588 at 609.664 kb on - strand, within HP15_588 at 609.664 kb on - strand, within HP15_588 at 609.747 kb on - strand, within HP15_588 at 609.862 kb on + strand at 609.875 kb on - strand at 610.050 kb on - strand at 610.086 kb on - strand at 610.123 kb on - strand at 610.272 kb on - strand at 610.284 kb on - strand at 610.301 kb on + strand, within HP15_589 at 610.388 kb on + strand, within HP15_589 at 610.474 kb on + strand, within HP15_589 at 610.474 kb on + strand, within HP15_589 at 610.482 kb on - strand, within HP15_589 at 610.483 kb on + strand, within HP15_589 at 610.530 kb on - strand, within HP15_589 at 610.584 kb on + strand, within HP15_589 at 610.599 kb on - strand, within HP15_589 at 610.604 kb on - strand, within HP15_589 at 610.644 kb on + strand, within HP15_589 at 610.780 kb on + strand, within HP15_589 at 610.790 kb on + strand, within HP15_589 at 610.790 kb on + strand, within HP15_589 at 610.790 kb on + strand, within HP15_589 at 610.790 kb on + strand, within HP15_589 at 610.824 kb on - strand, within HP15_589
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 607,958 + +1.5 607,963 + +0.7 607,973 + -2.7 607,976 + -0.6 607,991 + -1.2 608,989 + HP15_588 0.33 +0.4 609,029 - HP15_588 0.35 +1.6 609,087 + HP15_588 0.39 +1.2 609,095 - HP15_588 0.40 -0.0 609,095 - HP15_588 0.40 +2.5 609,095 - HP15_588 0.40 -0.3 609,095 - HP15_588 0.40 +1.5 609,140 + HP15_588 0.43 +1.1 609,191 - HP15_588 0.46 +2.1 609,307 + HP15_588 0.54 +1.6 609,404 - HP15_588 0.60 -0.1 609,426 - HP15_588 0.62 +0.4 609,426 - HP15_588 0.62 -1.0 609,634 + HP15_588 0.75 +2.5 609,664 - HP15_588 0.77 +0.3 609,664 - HP15_588 0.77 +0.0 609,747 - HP15_588 0.83 +1.8 609,862 + -0.2 609,875 - -0.4 610,050 - +1.2 610,086 - -0.5 610,123 - +1.3 610,272 - +1.5 610,284 - -0.3 610,301 + HP15_589 0.12 -0.8 610,388 + HP15_589 0.23 +2.1 610,474 + HP15_589 0.33 +0.1 610,474 + HP15_589 0.33 +0.7 610,482 - HP15_589 0.34 -0.5 610,483 + HP15_589 0.35 -1.4 610,530 - HP15_589 0.41 +0.5 610,584 + HP15_589 0.47 -2.9 610,599 - HP15_589 0.49 -0.6 610,604 - HP15_589 0.50 -0.9 610,644 + HP15_589 0.55 -0.7 610,780 + HP15_589 0.72 +2.4 610,790 + HP15_589 0.74 -0.1 610,790 + HP15_589 0.74 +1.5 610,790 + HP15_589 0.74 -0.8 610,790 + HP15_589 0.74 +1.4 610,824 - HP15_589 0.78 +1.8
Or see this region's nucleotide sequence