Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1002 and HP15_1003 are separated by 2 nucleotides HP15_1003 and HP15_1004 are separated by 14 nucleotides HP15_1004 and HP15_1005 are separated by 207 nucleotides
HP15_1002: HP15_1002 - methylcrotonyl-CoA carboxylase, alpha subunit, at 1,011,422 to 1,013,416
_1002
HP15_1003: HP15_1003 - enoyl-CoA hydratase/isomerase, at 1,013,419 to 1,014,225
_1003
HP15_1004: HP15_1004 - 3-methylcrotonyl CoA carboxylase, beta subunit, at 1,014,240 to 1,015,847
_1004
HP15_1005: HP15_1005 - acyl-CoA dehydrogenase domain protein, at 1,016,055 to 1,017,224
_1005
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1014.142 kb on + strand, within HP15_1003 at 1014.150 kb on - strand at 1014.166 kb on + strand at 1014.166 kb on + strand at 1014.299 kb on + strand at 1014.303 kb on - strand at 1014.307 kb on - strand at 1014.307 kb on - strand at 1014.321 kb on + strand at 1014.324 kb on - strand at 1014.329 kb on - strand at 1014.329 kb on - strand at 1014.334 kb on - strand at 1014.370 kb on + strand at 1014.374 kb on - strand at 1014.381 kb on + strand at 1014.381 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on - strand at 1014.389 kb on - strand at 1014.389 kb on - strand at 1014.517 kb on + strand, within HP15_1004 at 1014.540 kb on + strand, within HP15_1004 at 1014.544 kb on - strand, within HP15_1004 at 1014.559 kb on - strand, within HP15_1004 at 1014.632 kb on + strand, within HP15_1004 at 1014.632 kb on + strand, within HP15_1004 at 1014.642 kb on + strand, within HP15_1004 at 1014.649 kb on + strand, within HP15_1004 at 1014.714 kb on - strand, within HP15_1004 at 1014.714 kb on - strand, within HP15_1004 at 1014.877 kb on - strand, within HP15_1004 at 1014.883 kb on - strand, within HP15_1004 at 1014.910 kb on - strand, within HP15_1004 at 1016.364 kb on - strand, within HP15_1005
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,014,142 + HP15_1003 0.90 -1.8 1,014,150 - -1.3 1,014,166 + -1.0 1,014,166 + +0.2 1,014,299 + -0.3 1,014,303 - +1.5 1,014,307 - -0.2 1,014,307 - +0.5 1,014,321 + +1.8 1,014,324 - -1.3 1,014,329 - +1.2 1,014,329 - -1.4 1,014,334 - -1.0 1,014,370 + -0.7 1,014,374 - +0.3 1,014,381 + +1.0 1,014,381 + -1.3 1,014,389 + -2.7 1,014,389 + +2.0 1,014,389 - -0.8 1,014,389 - +0.2 1,014,389 - -0.8 1,014,517 + HP15_1004 0.17 -1.6 1,014,540 + HP15_1004 0.19 +1.6 1,014,544 - HP15_1004 0.19 -1.2 1,014,559 - HP15_1004 0.20 +1.4 1,014,632 + HP15_1004 0.24 -0.3 1,014,632 + HP15_1004 0.24 -2.1 1,014,642 + HP15_1004 0.25 -0.3 1,014,649 + HP15_1004 0.25 +2.0 1,014,714 - HP15_1004 0.29 -0.3 1,014,714 - HP15_1004 0.29 -0.5 1,014,877 - HP15_1004 0.40 +1.2 1,014,883 - HP15_1004 0.40 -0.1 1,014,910 - HP15_1004 0.42 +0.9 1,016,364 - HP15_1005 0.26 +1.1
Or see this region's nucleotide sequence