Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1000 and HP15_1001 are separated by 14 nucleotides HP15_1001 and HP15_1002 overlap by 1 nucleotides HP15_1002 and HP15_1003 are separated by 2 nucleotides HP15_1003 and HP15_1004 are separated by 14 nucleotides
HP15_1000: HP15_1000 - uncharacterized peroxidase-related protein, at 1,009,927 to 1,010,502
_1000
HP15_1001: HP15_1001 - pyruvate carboxyltransferase, at 1,010,517 to 1,011,422
_1001
HP15_1002: HP15_1002 - methylcrotonyl-CoA carboxylase, alpha subunit, at 1,011,422 to 1,013,416
_1002
HP15_1003: HP15_1003 - enoyl-CoA hydratase/isomerase, at 1,013,419 to 1,014,225
_1003
HP15_1004: HP15_1004 - 3-methylcrotonyl CoA carboxylase, beta subunit, at 1,014,240 to 1,015,847
_1004
Position (kb)
1011
1012
1013
1014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1014.142 kb on + strand, within HP15_1003 at 1014.150 kb on - strand at 1014.166 kb on + strand at 1014.166 kb on + strand at 1014.299 kb on + strand at 1014.303 kb on - strand at 1014.307 kb on - strand at 1014.307 kb on - strand at 1014.321 kb on + strand at 1014.324 kb on - strand at 1014.329 kb on - strand at 1014.329 kb on - strand at 1014.334 kb on - strand at 1014.370 kb on + strand at 1014.374 kb on - strand at 1014.381 kb on + strand at 1014.381 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on + strand at 1014.389 kb on - strand at 1014.389 kb on - strand at 1014.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,014,142 + HP15_1003 0.90 -1.8 1,014,150 - -1.3 1,014,166 + -1.0 1,014,166 + +0.2 1,014,299 + -0.3 1,014,303 - +1.5 1,014,307 - -0.2 1,014,307 - +0.5 1,014,321 + +1.8 1,014,324 - -1.3 1,014,329 - +1.2 1,014,329 - -1.4 1,014,334 - -1.0 1,014,370 + -0.7 1,014,374 - +0.3 1,014,381 + +1.0 1,014,381 + -1.3 1,014,389 + -2.7 1,014,389 + +2.0 1,014,389 - -0.8 1,014,389 - +0.2 1,014,389 - -0.8
Or see this region's nucleotide sequence